Induction of Hyperthyroxinemia in Balb/C but not in Several Other Strains of Mice
We recently expressed the extracellular domain of the human TSHR (ETSHR) protein using a baculovirus expression system and purified it to homogeneity. The ETSHR specifically binds both TSH and antibodies to TSHR. In the present study, C57BL/6J, SJL/J, BALB/cJ and BlOBR.SgSnJ mice were immunized with...
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| Veröffentlicht in: | Autoimmunity (Chur, Switzerland) Switzerland), 1994, Vol.18 (2), p.103-112 |
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| creator | Wagle, Neelam M. Dallas, John S. Seetharamaiah, Gattadahalli S. Fan, Ji-Lao Desai, Rajesh K. Memar, Omeed Rajaraman, Srinivasan Prabhakar, Bellur S. |
| description | We recently expressed the extracellular domain of the human TSHR (ETSHR) protein using a baculovirus expression system and purified it to homogeneity. The ETSHR specifically binds both TSH and antibodies to TSHR. In the present study, C57BL/6J, SJL/J, BALB/cJ and BlOBR.SgSnJ mice were immunized with the recombinant ETSHR or an equivalent amount of control antigen. All strains of mice produced high titers of antibody against the TSHR protein which were capable of blocking the binding of TSH to native TSHR. However, only BALB/cJ mice showed significantly elevated levels of thyroxine in their sera compared to the control mice. Similarly, BALB/cJ mice primed with ETSHR and then challenged with thyroid membranes showed significantly elevated levels of thyroxine. In addition, histopathological examination of thyroid glands from affected mice showed morphological changes characterized by hydropic and subnuclear vacuolar changes and focal scalloping, with no apparent inflammation or glandular destruction. Moreover, mice with elevated thyroxine levels showed increased in vivo thyroidal uptake of l31 Iodine. Together, these data suggest that BALB/cJ mice are susceptible to the induction of hyperthyroxinemia. |
| doi_str_mv | 10.3109/08916939409007983 |
| format | Article |
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The ETSHR specifically binds both TSH and antibodies to TSHR. In the present study, C57BL/6J, SJL/J, BALB/cJ and BlOBR.SgSnJ mice were immunized with the recombinant ETSHR or an equivalent amount of control antigen. All strains of mice produced high titers of antibody against the TSHR protein which were capable of blocking the binding of TSH to native TSHR. However, only BALB/cJ mice showed significantly elevated levels of thyroxine in their sera compared to the control mice. Similarly, BALB/cJ mice primed with ETSHR and then challenged with thyroid membranes showed significantly elevated levels of thyroxine. In addition, histopathological examination of thyroid glands from affected mice showed morphological changes characterized by hydropic and subnuclear vacuolar changes and focal scalloping, with no apparent inflammation or glandular destruction. Moreover, mice with elevated thyroxine levels showed increased in vivo thyroidal uptake of l31 Iodine. Together, these data suggest that BALB/cJ mice are susceptible to the induction of hyperthyroxinemia.</description><identifier>ISSN: 0891-6934</identifier><identifier>EISSN: 1607-842X</identifier><identifier>DOI: 10.3109/08916939409007983</identifier><identifier>PMID: 7742472</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Antibodies - analysis ; Antibody Formation ; Binding, Competitive ; Cell Membrane - immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Graves' disease ; Hyperthyroxinemia ; Hyperthyroxinemia - immunology ; Immunization ; Immunoglobulins, Thyroid-Stimulating - blood ; Immunoglobulins, Thyroid-Stimulating - immunology ; Mice ; Mice, Inbred BALB C - immunology ; Mice, Inbred C57BL ; Mice, Inbred Strains ; Receptors, Thyrotropin - immunology ; Recombinant Proteins - immunology ; Thyroid Gland - immunology ; Thyroid Gland - pathology ; Thyrotropin receptor ; Thyroxine - blood</subject><ispartof>Autoimmunity (Chur, Switzerland), 1994, Vol.18 (2), p.103-112</ispartof><rights>1994 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-2fb25968a1a05942d1f7e1f18aaa50918042fbe01441c377d8b70611d83bcf753</citedby><cites>FETCH-LOGICAL-c401t-2fb25968a1a05942d1f7e1f18aaa50918042fbe01441c377d8b70611d83bcf753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/08916939409007983$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/08916939409007983$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,4022,27922,27923,27924,59646,60435,61220,61401</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7742472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wagle, Neelam M.</creatorcontrib><creatorcontrib>Dallas, John S.</creatorcontrib><creatorcontrib>Seetharamaiah, Gattadahalli S.</creatorcontrib><creatorcontrib>Fan, Ji-Lao</creatorcontrib><creatorcontrib>Desai, Rajesh K.</creatorcontrib><creatorcontrib>Memar, Omeed</creatorcontrib><creatorcontrib>Rajaraman, Srinivasan</creatorcontrib><creatorcontrib>Prabhakar, Bellur S.</creatorcontrib><title>Induction of Hyperthyroxinemia in Balb/C but not in Several Other Strains of Mice</title><title>Autoimmunity (Chur, Switzerland)</title><addtitle>Autoimmunity</addtitle><description>We recently expressed the extracellular domain of the human TSHR (ETSHR) protein using a baculovirus expression system and purified it to homogeneity. The ETSHR specifically binds both TSH and antibodies to TSHR. In the present study, C57BL/6J, SJL/J, BALB/cJ and BlOBR.SgSnJ mice were immunized with the recombinant ETSHR or an equivalent amount of control antigen. All strains of mice produced high titers of antibody against the TSHR protein which were capable of blocking the binding of TSH to native TSHR. However, only BALB/cJ mice showed significantly elevated levels of thyroxine in their sera compared to the control mice. Similarly, BALB/cJ mice primed with ETSHR and then challenged with thyroid membranes showed significantly elevated levels of thyroxine. In addition, histopathological examination of thyroid glands from affected mice showed morphological changes characterized by hydropic and subnuclear vacuolar changes and focal scalloping, with no apparent inflammation or glandular destruction. Moreover, mice with elevated thyroxine levels showed increased in vivo thyroidal uptake of l31 Iodine. Together, these data suggest that BALB/cJ mice are susceptible to the induction of hyperthyroxinemia.</description><subject>Animals</subject><subject>Antibodies - analysis</subject><subject>Antibody Formation</subject><subject>Binding, Competitive</subject><subject>Cell Membrane - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Graves' disease</subject><subject>Hyperthyroxinemia</subject><subject>Hyperthyroxinemia - immunology</subject><subject>Immunization</subject><subject>Immunoglobulins, Thyroid-Stimulating - blood</subject><subject>Immunoglobulins, Thyroid-Stimulating - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C - immunology</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred Strains</subject><subject>Receptors, Thyrotropin - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Thyroid Gland - immunology</subject><subject>Thyroid Gland - pathology</subject><subject>Thyrotropin receptor</subject><subject>Thyroxine - blood</subject><issn>0891-6934</issn><issn>1607-842X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFFr2zAUhUXpSNOuP6APBT_1zcuVrVgW28satqbQUko22Ju5liWiYkuZJK_zv69NQqGM9Ulwz_k-xCHkgsKnnIJYQCloIXLBQABwUeZHZE4L4GnJsl_HZD7l6VhgJ-Q0hCcAyHjBZmTGOcsYz-bk8dY2vYzG2cTpZD3slI_bwbu_xqrOYGJsco1tvVgldR8T6-J02ag_ymObPMSt8skmejQ2TPy9keoj-aCxDer88J6Rn9-__Vit07uHm9vV17tUMqAxzXSdLUVRIkVYCpY1VHNFNS0RcQmClsDGigLKGJU5501Zcygobcq8lpov8zNytffuvPvdqxCrzgSp2hatcn2oOAdRsGIq0n1ReheCV7raedOhHyoK1TRj9c-MI3N5kPd1p5pX4rDbmH_Z58Zq5zt8dr5tqohD67z2aKUJk_r_-s9v8K3CNm4lelU9ud7bcbd3PvcCKLKRYQ</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Wagle, Neelam M.</creator><creator>Dallas, John S.</creator><creator>Seetharamaiah, Gattadahalli S.</creator><creator>Fan, Ji-Lao</creator><creator>Desai, Rajesh K.</creator><creator>Memar, Omeed</creator><creator>Rajaraman, Srinivasan</creator><creator>Prabhakar, Bellur S.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Induction of Hyperthyroxinemia in Balb/C but not in Several Other Strains of Mice</title><author>Wagle, Neelam M. ; Dallas, John S. ; Seetharamaiah, Gattadahalli S. ; Fan, Ji-Lao ; Desai, Rajesh K. ; Memar, Omeed ; Rajaraman, Srinivasan ; Prabhakar, Bellur S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-2fb25968a1a05942d1f7e1f18aaa50918042fbe01441c377d8b70611d83bcf753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Antibodies - analysis</topic><topic>Antibody Formation</topic><topic>Binding, Competitive</topic><topic>Cell Membrane - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Graves' disease</topic><topic>Hyperthyroxinemia</topic><topic>Hyperthyroxinemia - immunology</topic><topic>Immunization</topic><topic>Immunoglobulins, Thyroid-Stimulating - blood</topic><topic>Immunoglobulins, Thyroid-Stimulating - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C - immunology</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred Strains</topic><topic>Receptors, Thyrotropin - immunology</topic><topic>Recombinant Proteins - immunology</topic><topic>Thyroid Gland - immunology</topic><topic>Thyroid Gland - pathology</topic><topic>Thyrotropin receptor</topic><topic>Thyroxine - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wagle, Neelam M.</creatorcontrib><creatorcontrib>Dallas, John S.</creatorcontrib><creatorcontrib>Seetharamaiah, Gattadahalli S.</creatorcontrib><creatorcontrib>Fan, Ji-Lao</creatorcontrib><creatorcontrib>Desai, Rajesh K.</creatorcontrib><creatorcontrib>Memar, Omeed</creatorcontrib><creatorcontrib>Rajaraman, Srinivasan</creatorcontrib><creatorcontrib>Prabhakar, Bellur S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Autoimmunity (Chur, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wagle, Neelam M.</au><au>Dallas, John S.</au><au>Seetharamaiah, Gattadahalli S.</au><au>Fan, Ji-Lao</au><au>Desai, Rajesh K.</au><au>Memar, Omeed</au><au>Rajaraman, Srinivasan</au><au>Prabhakar, Bellur S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of Hyperthyroxinemia in Balb/C but not in Several Other Strains of Mice</atitle><jtitle>Autoimmunity (Chur, Switzerland)</jtitle><addtitle>Autoimmunity</addtitle><date>1994</date><risdate>1994</risdate><volume>18</volume><issue>2</issue><spage>103</spage><epage>112</epage><pages>103-112</pages><issn>0891-6934</issn><eissn>1607-842X</eissn><abstract>We recently expressed the extracellular domain of the human TSHR (ETSHR) protein using a baculovirus expression system and purified it to homogeneity. The ETSHR specifically binds both TSH and antibodies to TSHR. In the present study, C57BL/6J, SJL/J, BALB/cJ and BlOBR.SgSnJ mice were immunized with the recombinant ETSHR or an equivalent amount of control antigen. All strains of mice produced high titers of antibody against the TSHR protein which were capable of blocking the binding of TSH to native TSHR. However, only BALB/cJ mice showed significantly elevated levels of thyroxine in their sera compared to the control mice. Similarly, BALB/cJ mice primed with ETSHR and then challenged with thyroid membranes showed significantly elevated levels of thyroxine. In addition, histopathological examination of thyroid glands from affected mice showed morphological changes characterized by hydropic and subnuclear vacuolar changes and focal scalloping, with no apparent inflammation or glandular destruction. Moreover, mice with elevated thyroxine levels showed increased in vivo thyroidal uptake of l31 Iodine. 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| ispartof | Autoimmunity (Chur, Switzerland), 1994, Vol.18 (2), p.103-112 |
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| language | eng |
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| source | MEDLINE; Taylor & Francis:Master (3349 titles) |
| subjects | Animals Antibodies - analysis Antibody Formation Binding, Competitive Cell Membrane - immunology Enzyme-Linked Immunosorbent Assay Female Graves' disease Hyperthyroxinemia Hyperthyroxinemia - immunology Immunization Immunoglobulins, Thyroid-Stimulating - blood Immunoglobulins, Thyroid-Stimulating - immunology Mice Mice, Inbred BALB C - immunology Mice, Inbred C57BL Mice, Inbred Strains Receptors, Thyrotropin - immunology Recombinant Proteins - immunology Thyroid Gland - immunology Thyroid Gland - pathology Thyrotropin receptor Thyroxine - blood |
| title | Induction of Hyperthyroxinemia in Balb/C but not in Several Other Strains of Mice |
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