Antibodies to Escherichia coli 06 Porins Cross-React with Urinary Pathogens
Antibodies to partially purified E. coli 06 35–40 KDa porin trimers recognized the reactive epitopes in the intact porin surface molecule present in various wild-type, heterologous, urinary pathogens. The presence of lipopolysaccharide in the membrane did not shield the antibody binding sites. The r...
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| Veröffentlicht in: | Immunobiology (1979) 1994-12, Vol.192 (1), p.65-76 |
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| container_title | Immunobiology (1979) |
| container_volume | 192 |
| creator | Kawahara, Motoshi Human, Liset G. Winningham, Jefferey S. Domingue, Gerald J. |
| description | Antibodies to partially purified
E. coli 06 35–40 KDa porin trimers recognized the reactive epitopes in the intact porin surface molecule present in various wild-type, heterologous, urinary pathogens. The presence of lipopolysaccharide in the membrane did not shield the antibody binding sites. The reactivity was shown to be specific for porins since LPS-absorbed porin antisera reacted with porins on immunoblots and showed no reactivity with LPS. Additionally, the cross-reactions were abolished by absorption of the porin antisera with
E. coli 06 containing porin trimers. These data strengthen the rationale for exploring the enhancement of immunoprotection by monoclonal antibodies to specific immunoreactive antigens in the porin molecule. |
| doi_str_mv | 10.1016/S0171-2985(11)80408-0 |
| format | Article |
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E. coli 06 35–40 KDa porin trimers recognized the reactive epitopes in the intact porin surface molecule present in various wild-type, heterologous, urinary pathogens. The presence of lipopolysaccharide in the membrane did not shield the antibody binding sites. The reactivity was shown to be specific for porins since LPS-absorbed porin antisera reacted with porins on immunoblots and showed no reactivity with LPS. Additionally, the cross-reactions were abolished by absorption of the porin antisera with
E. coli 06 containing porin trimers. These data strengthen the rationale for exploring the enhancement of immunoprotection by monoclonal antibodies to specific immunoreactive antigens in the porin molecule.</description><identifier>ISSN: 0171-2985</identifier><identifier>EISSN: 1878-3279</identifier><identifier>DOI: 10.1016/S0171-2985(11)80408-0</identifier><identifier>PMID: 7538488</identifier><language>eng</language><publisher>Netherlands: Elsevier GmbH</publisher><subject>Animals ; Antibodies, Bacterial - immunology ; Bacteria - immunology ; Bacteria - isolation & purification ; Binding Sites, Antibody - immunology ; Cross Reactions ; Enzyme-Linked Immunosorbent Assay ; Epitopes - immunology ; Escherichia coli - immunology ; Escherichia coli - isolation & purification ; Fluorescent Antibody Technique ; Humans ; Lipopolysaccharides - immunology ; Porins - immunology ; Rabbits ; Urinary Tract Infections - microbiology ; Urine - microbiology</subject><ispartof>Immunobiology (1979), 1994-12, Vol.192 (1), p.65-76</ispartof><rights>1994 Gustav Fischer Verlag · Stuttgart · Jena · New York</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-67a935f4b5fc32a93af3227cd65b4ac94b3dbff7bac0c6c51109cf992acf2b153</citedby><cites>FETCH-LOGICAL-c360t-67a935f4b5fc32a93af3227cd65b4ac94b3dbff7bac0c6c51109cf992acf2b153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0171-2985(11)80408-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7538488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawahara, Motoshi</creatorcontrib><creatorcontrib>Human, Liset G.</creatorcontrib><creatorcontrib>Winningham, Jefferey S.</creatorcontrib><creatorcontrib>Domingue, Gerald J.</creatorcontrib><title>Antibodies to Escherichia coli 06 Porins Cross-React with Urinary Pathogens</title><title>Immunobiology (1979)</title><addtitle>Immunobiology</addtitle><description>Antibodies to partially purified
E. coli 06 35–40 KDa porin trimers recognized the reactive epitopes in the intact porin surface molecule present in various wild-type, heterologous, urinary pathogens. The presence of lipopolysaccharide in the membrane did not shield the antibody binding sites. The reactivity was shown to be specific for porins since LPS-absorbed porin antisera reacted with porins on immunoblots and showed no reactivity with LPS. Additionally, the cross-reactions were abolished by absorption of the porin antisera with
E. coli 06 containing porin trimers. These data strengthen the rationale for exploring the enhancement of immunoprotection by monoclonal antibodies to specific immunoreactive antigens in the porin molecule.</description><subject>Animals</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Bacteria - immunology</subject><subject>Bacteria - isolation & purification</subject><subject>Binding Sites, Antibody - immunology</subject><subject>Cross Reactions</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes - immunology</subject><subject>Escherichia coli - immunology</subject><subject>Escherichia coli - isolation & purification</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>Lipopolysaccharides - immunology</subject><subject>Porins - immunology</subject><subject>Rabbits</subject><subject>Urinary Tract Infections - microbiology</subject><subject>Urine - microbiology</subject><issn>0171-2985</issn><issn>1878-3279</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotVZ_QiEn0cNqkv1IcpJS6gcWLGrPITubuJF2U5Ot4r93-0GvnmaYeWfemQehISU3lNDi9o1QThMmRX5F6bUgGREJOUJ9KrhIUsblMeofJKfoLMZPQqhkXPRQj-epyIToo-dR07rSV85E3Ho8iVCb4KB2GoNfOEwKPPPBNRGPg48xeTUaWvzj2hrPu7IOv3im29p_mCaeoxOrF9Fc7OMAze8n7-PHZPry8DQeTRNIC9ImBdcyzW1W5hZS1uXapoxxqIq8zDTIrEyr0lpeaiBQQE4pkWClZBosK2meDtDlbu8q-K-1ia1aughmsdCN8euoOCeSESY7Yb4Twub2YKxaBbfsblaUqA1EtYWoNoQUpWoLUZFubrg3WJdLUx2m9tS6_t2ub7ovv50JKoIzDZjKBQOtqrz7x-EPmxeBXQ</recordid><startdate>19941201</startdate><enddate>19941201</enddate><creator>Kawahara, Motoshi</creator><creator>Human, Liset G.</creator><creator>Winningham, Jefferey S.</creator><creator>Domingue, Gerald J.</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941201</creationdate><title>Antibodies to Escherichia coli 06 Porins Cross-React with Urinary Pathogens</title><author>Kawahara, Motoshi ; Human, Liset G. ; Winningham, Jefferey S. ; Domingue, Gerald J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-67a935f4b5fc32a93af3227cd65b4ac94b3dbff7bac0c6c51109cf992acf2b153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Bacteria - immunology</topic><topic>Bacteria - isolation & purification</topic><topic>Binding Sites, Antibody - immunology</topic><topic>Cross Reactions</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes - immunology</topic><topic>Escherichia coli - immunology</topic><topic>Escherichia coli - isolation & purification</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>Lipopolysaccharides - immunology</topic><topic>Porins - immunology</topic><topic>Rabbits</topic><topic>Urinary Tract Infections - microbiology</topic><topic>Urine - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawahara, Motoshi</creatorcontrib><creatorcontrib>Human, Liset G.</creatorcontrib><creatorcontrib>Winningham, Jefferey S.</creatorcontrib><creatorcontrib>Domingue, Gerald J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Immunobiology (1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawahara, Motoshi</au><au>Human, Liset G.</au><au>Winningham, Jefferey S.</au><au>Domingue, Gerald J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibodies to Escherichia coli 06 Porins Cross-React with Urinary Pathogens</atitle><jtitle>Immunobiology (1979)</jtitle><addtitle>Immunobiology</addtitle><date>1994-12-01</date><risdate>1994</risdate><volume>192</volume><issue>1</issue><spage>65</spage><epage>76</epage><pages>65-76</pages><issn>0171-2985</issn><eissn>1878-3279</eissn><abstract>Antibodies to partially purified
E. coli 06 35–40 KDa porin trimers recognized the reactive epitopes in the intact porin surface molecule present in various wild-type, heterologous, urinary pathogens. The presence of lipopolysaccharide in the membrane did not shield the antibody binding sites. The reactivity was shown to be specific for porins since LPS-absorbed porin antisera reacted with porins on immunoblots and showed no reactivity with LPS. Additionally, the cross-reactions were abolished by absorption of the porin antisera with
E. coli 06 containing porin trimers. These data strengthen the rationale for exploring the enhancement of immunoprotection by monoclonal antibodies to specific immunoreactive antigens in the porin molecule.</abstract><cop>Netherlands</cop><pub>Elsevier GmbH</pub><pmid>7538488</pmid><doi>10.1016/S0171-2985(11)80408-0</doi><tpages>12</tpages></addata></record> |
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| ispartof | Immunobiology (1979), 1994-12, Vol.192 (1), p.65-76 |
| issn | 0171-2985 1878-3279 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Animals Antibodies, Bacterial - immunology Bacteria - immunology Bacteria - isolation & purification Binding Sites, Antibody - immunology Cross Reactions Enzyme-Linked Immunosorbent Assay Epitopes - immunology Escherichia coli - immunology Escherichia coli - isolation & purification Fluorescent Antibody Technique Humans Lipopolysaccharides - immunology Porins - immunology Rabbits Urinary Tract Infections - microbiology Urine - microbiology |
| title | Antibodies to Escherichia coli 06 Porins Cross-React with Urinary Pathogens |
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