Rapid purification of adenylate kinase from human erythrocytes and skeletal muscle

Adenylate kinase from human erythrocytes and skeletal muscle can be purified to homogeneity by a new procedure based on DEAE-Sepharose and Blue Sepharose affinity chromatography and Sephadex G-75 fractionation. For the enzyme purified from erythrocytes the specific activity is 3000 U/mg of protein,...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1986-10, Vol.250 (1), p.19-22
1. Verfasser:	Nealon, Daniel A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenylate Kinase - blood Adenylate Kinase - isolation & purification Applied sciences Chromatography, Affinity Chromatography, Gel Electrophoresis, Polyacrylamide Gel Erythrocytes - enzymology Exact sciences and technology Humans Muscles - enzymology Other techniques and industries Phosphotransferases - isolation & purification
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