Danazol binds to progesterone receptors and inhibits the growth of human endometrial cancer cells in vitro
Based on our recent findings that danazol, an isoxazol derivative of ethinyltestosterone, has a profound growth-inhibitory effect on an established human endometrial adenocarcinoma cell line, the effects of danazol on cancer cells from human endometrial adenocarcinomas obtained by hysterectomy were...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 1986-10, Vol.155 (4), p.857-861 |
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| container_end_page | 861 |
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| container_issue | 4 |
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| container_title | American journal of obstetrics and gynecology |
| container_volume | 155 |
| creator | Ikegami, Hiromasa Terakawa, Naoki Shimizu, Ikuya Kano, Hideo Tanaka, Yoshiaki Aono, Toshihiro Tanizawa, Osamu Matsumoto, Keishi |
| description | Based on our recent findings that danazol, an isoxazol derivative of ethinyltestosterone, has a profound growth-inhibitory effect on an established human endometrial adenocarcinoma cell line, the effects of danazol on cancer cells from human endometrial adenocarcinomas obtained by hysterectomy were investigated in the present study. Of the 22 uterine adenocarcinomas, estrogen, progesterone, and androgen receptors were found in 12, 14, and 4 tumors, respectively. Competitive binding studies showed that danazol specifically binds to progesterone and androgen receptors but not to estrogen receptors. Of the five cancer cells from five patients succeeded in primary cell culture, a marked inhibition of cell growth was demonstrated by addition of danazol in two cancer cells having progesterone but not androgen receptors. However, danazol did not affect the growth of the remaining three cancer cells lacking progesterone receptors. These results strongly suggest that danazol has a significant growth-inhibitory effect on human endometrial adenocarcinoma cells, possibly through progesterone receptors in the cells. |
| doi_str_mv | 10.1016/S0002-9378(86)80039-4 |
| format | Article |
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Of the 22 uterine adenocarcinomas, estrogen, progesterone, and androgen receptors were found in 12, 14, and 4 tumors, respectively. Competitive binding studies showed that danazol specifically binds to progesterone and androgen receptors but not to estrogen receptors. Of the five cancer cells from five patients succeeded in primary cell culture, a marked inhibition of cell growth was demonstrated by addition of danazol in two cancer cells having progesterone but not androgen receptors. However, danazol did not affect the growth of the remaining three cancer cells lacking progesterone receptors. These results strongly suggest that danazol has a significant growth-inhibitory effect on human endometrial adenocarcinoma cells, possibly through progesterone receptors in the cells.</description><identifier>ISSN: 0002-9378</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/S0002-9378(86)80039-4</identifier><identifier>PMID: 3766641</identifier><identifier>CODEN: AJOGAH</identifier><language>eng</language><publisher>Philadelphia, PA: Elsevier Inc</publisher><subject>Adenocarcinoma - metabolism ; Adenocarcinoma - physiopathology ; Adult ; Animal tumors. 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Of the 22 uterine adenocarcinomas, estrogen, progesterone, and androgen receptors were found in 12, 14, and 4 tumors, respectively. Competitive binding studies showed that danazol specifically binds to progesterone and androgen receptors but not to estrogen receptors. Of the five cancer cells from five patients succeeded in primary cell culture, a marked inhibition of cell growth was demonstrated by addition of danazol in two cancer cells having progesterone but not androgen receptors. However, danazol did not affect the growth of the remaining three cancer cells lacking progesterone receptors. These results strongly suggest that danazol has a significant growth-inhibitory effect on human endometrial adenocarcinoma cells, possibly through progesterone receptors in the cells.</description><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - physiopathology</subject><subject>Adult</subject><subject>Animal tumors. Experimental tumors</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>Danazol</subject><subject>Danazol - metabolism</subject><subject>Danazol - pharmacology</subject><subject>endometrial cancer</subject><subject>Experimental genital and mammary tumors</subject><subject>Female</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Pregnadienes - metabolism</subject><subject>progesterone receptors</subject><subject>Receptors, Androgen - metabolism</subject><subject>Receptors, Estrogen - metabolism</subject><subject>Receptors, Progesterone - metabolism</subject><subject>Thymidine - metabolism</subject><subject>Tumors</subject><subject>Uterine Neoplasms - metabolism</subject><subject>Uterine Neoplasms - physiopathology</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMluHCEURVGUyGkPn2CJhRXFi0qgBoZVZDmjZMmL2GtEwcONVQVtoB0lX2_K3eptVgi9c-Hdg9A5JZ8ooezzb0JI28iOi4-CXQpCOtn0b9CKEskbJph4i1YH5D06zvlxubayPUJHHWeM9XSFHr_qoP_FCY8-2IxLxJsUHyAXSDEATmBgU2LKWAeLfVj70ZeKrQE_pPinrHF0eL2ddcAQbJyhJK8nbHQwkLCBaco1hZ99SfEUvXN6ynC2P0_Q_fdvd9c_m5vbH7-ur24a05G-NIYwC3yU1IIWzoysNc72Ro9yEFKyWnTkY-uMoSAGcHYwtpdicFK0RApHuxP0YfdubfK0rVXU7POyig4Qt1lxTniFF3DYgSbFnBM4tUl-1umvokQtjtWrY7UIVIKpV8eqr7nz_QfbcQZ7SO2l1vnFfq6z0ZNL1YbPB0xQ3raMVezLDoMq49lDUtl4qOKsr9qLstH_Z5EXssqa3g</recordid><startdate>198610</startdate><enddate>198610</enddate><creator>Ikegami, Hiromasa</creator><creator>Terakawa, Naoki</creator><creator>Shimizu, Ikuya</creator><creator>Kano, Hideo</creator><creator>Tanaka, Yoshiaki</creator><creator>Aono, Toshihiro</creator><creator>Tanizawa, Osamu</creator><creator>Matsumoto, Keishi</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198610</creationdate><title>Danazol binds to progesterone receptors and inhibits the growth of human endometrial cancer cells in vitro</title><author>Ikegami, Hiromasa ; Terakawa, Naoki ; Shimizu, Ikuya ; Kano, Hideo ; Tanaka, Yoshiaki ; Aono, Toshihiro ; Tanizawa, Osamu ; Matsumoto, Keishi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c304t-c06de7b91dea8fcb62cfd4cab958996800b7b2fcc1e85efd5cd4985f982098f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - physiopathology</topic><topic>Adult</topic><topic>Animal tumors. Experimental tumors</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>Danazol</topic><topic>Danazol - metabolism</topic><topic>Danazol - pharmacology</topic><topic>endometrial cancer</topic><topic>Experimental genital and mammary tumors</topic><topic>Female</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Pregnadienes - metabolism</topic><topic>progesterone receptors</topic><topic>Receptors, Androgen - metabolism</topic><topic>Receptors, Estrogen - metabolism</topic><topic>Receptors, Progesterone - metabolism</topic><topic>Thymidine - metabolism</topic><topic>Tumors</topic><topic>Uterine Neoplasms - metabolism</topic><topic>Uterine Neoplasms - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ikegami, Hiromasa</creatorcontrib><creatorcontrib>Terakawa, Naoki</creatorcontrib><creatorcontrib>Shimizu, Ikuya</creatorcontrib><creatorcontrib>Kano, Hideo</creatorcontrib><creatorcontrib>Tanaka, Yoshiaki</creatorcontrib><creatorcontrib>Aono, Toshihiro</creatorcontrib><creatorcontrib>Tanizawa, Osamu</creatorcontrib><creatorcontrib>Matsumoto, Keishi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ikegami, Hiromasa</au><au>Terakawa, Naoki</au><au>Shimizu, Ikuya</au><au>Kano, Hideo</au><au>Tanaka, Yoshiaki</au><au>Aono, Toshihiro</au><au>Tanizawa, Osamu</au><au>Matsumoto, Keishi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Danazol binds to progesterone receptors and inhibits the growth of human endometrial cancer cells in vitro</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>1986-10</date><risdate>1986</risdate><volume>155</volume><issue>4</issue><spage>857</spage><epage>861</epage><pages>857-861</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><coden>AJOGAH</coden><abstract>Based on our recent findings that danazol, an isoxazol derivative of ethinyltestosterone, has a profound growth-inhibitory effect on an established human endometrial adenocarcinoma cell line, the effects of danazol on cancer cells from human endometrial adenocarcinomas obtained by hysterectomy were investigated in the present study. Of the 22 uterine adenocarcinomas, estrogen, progesterone, and androgen receptors were found in 12, 14, and 4 tumors, respectively. Competitive binding studies showed that danazol specifically binds to progesterone and androgen receptors but not to estrogen receptors. Of the five cancer cells from five patients succeeded in primary cell culture, a marked inhibition of cell growth was demonstrated by addition of danazol in two cancer cells having progesterone but not androgen receptors. However, danazol did not affect the growth of the remaining three cancer cells lacking progesterone receptors. These results strongly suggest that danazol has a significant growth-inhibitory effect on human endometrial adenocarcinoma cells, possibly through progesterone receptors in the cells.</abstract><cop>Philadelphia, PA</cop><pub>Elsevier Inc</pub><pmid>3766641</pmid><doi>10.1016/S0002-9378(86)80039-4</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0002-9378 |
| ispartof | American journal of obstetrics and gynecology, 1986-10, Vol.155 (4), p.857-861 |
| issn | 0002-9378 1097-6868 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adenocarcinoma - metabolism Adenocarcinoma - physiopathology Adult Animal tumors. Experimental tumors Binding, Competitive Biological and medical sciences Cell Division - drug effects Cells, Cultured Danazol Danazol - metabolism Danazol - pharmacology endometrial cancer Experimental genital and mammary tumors Female Humans Medical sciences Middle Aged Pregnadienes - metabolism progesterone receptors Receptors, Androgen - metabolism Receptors, Estrogen - metabolism Receptors, Progesterone - metabolism Thymidine - metabolism Tumors Uterine Neoplasms - metabolism Uterine Neoplasms - physiopathology |
| title | Danazol binds to progesterone receptors and inhibits the growth of human endometrial cancer cells in vitro |
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