Rat Monoclonal Antibodies Differentiating between the Epstein-Barr Virus Nuclear Antigens 2A (EBNA2A) and 2B (EBNA2B)
Rat monoclonal antibodies were produced against the C-terminus of Epstein-Barr virus nuclear antigens 2A (EBNA2A) and 28 (EBNA2B) expressed as bacterial trpE fusion proteins The initial screening was performed using a soluble bacterial extract containing the fusion proteins. Positive hybridomas were...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1995-04, Vol.208 (1), p.336-342 |
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| creator | Kremmer, Elisabeth Kranz, Bernd R. Hille, Annette Klein, Karin Eulitz, Manfred Hoffmann-Fezer, Gertrud Feiden, Wolfgang Herrmann, Katharina Delecluse, Henri-Jacques Delsol, George Bornkamm, Georg W. Mueller-Lantzsch, Nikolaus Grässer, Friedrich A. |
| description | Rat monoclonal antibodies were produced against the C-terminus of Epstein-Barr virus nuclear antigens 2A (EBNA2A) and 28 (EBNA2B) expressed as bacterial trpE fusion proteins The initial screening was performed using a soluble bacterial extract containing the fusion proteins. Positive hybridomas were confirmed by immunofluorescence on SF158 (Spodoptera frugiperda) insect cells infected with recombinant baculovirus (Autographa californica nuclear polyhedrosis virus) and expressing the complete EBNA2A or EBNA2B genes. We selected a panel of antibodies which reacted either with both antigens or specifically with EBNA2A or with EBNA2B. The antibodies were extensively characterized using immunoprecipitation. Western blotting, epitope mapping on synthesized peptide segments of EBNA2A, immunocytology, and immunohistology on both cryostat sections and paraffin sections of AIDS-associated primary central nervous system lymphomas. |
| doi_str_mv | 10.1006/viro.1995.1157 |
| format | Article |
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Western blotting, epitope mapping on synthesized peptide segments of EBNA2A, immunocytology, and immunohistology on both cryostat sections and paraffin sections of AIDS-associated primary central nervous system lymphomas.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Monoclonal - isolation & purification</subject><subject>Antibody Specificity</subject><subject>Epitope Mapping</subject><subject>Epstein-Barr virus</subject><subject>Epstein-Barr Virus Nuclear Antigens - immunology</subject><subject>Hybridomas</subject><subject>Rats</subject><subject>Viral Proteins</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQRi1ERbeFK0fkE6KHLB4nsTfHbNkCUlskBFwtxxkXo6y9tZ0i_n297EqcUE_jsd58h-8R8hrYEhgT7x9cDEvounYJ0MpnZAGsExWrG3hOFow1vBIrzk_JWUq_WNmlZC_IKcCqBgliQeavOtOb4IOZgtcT7X12QxgdJvrBWYsRy4fOzt_RAfNvRE_zT6SbXcrofLXWMdIfLs6J3s5mQh3_JtyhT5T39N1mfdvz_oJqP1K-Pu7ri5fkxOop4avjPCffrzbfLj9V118-fr7sryvTAORKskbX3LZcD7VuJG9HycTAWz4Ka41pRXnWYpDCoEXGDZOCodEtWoE4yLY-J28PubsY7mdMWW1dMjhN2mOYkyptyFUH9ZMglBYFdLyAywNoYkgpolW76LY6_lHA1N6I2htReyNqb6QcvDkmz8MWx3_4UUEBVgcASxEPDqNKxqE3OLqIJqsxuP9lPwLuhJhu</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>Kremmer, Elisabeth</creator><creator>Kranz, Bernd R.</creator><creator>Hille, Annette</creator><creator>Klein, Karin</creator><creator>Eulitz, Manfred</creator><creator>Hoffmann-Fezer, Gertrud</creator><creator>Feiden, Wolfgang</creator><creator>Herrmann, Katharina</creator><creator>Delecluse, Henri-Jacques</creator><creator>Delsol, George</creator><creator>Bornkamm, Georg W.</creator><creator>Mueller-Lantzsch, Nikolaus</creator><creator>Grässer, Friedrich A.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19950401</creationdate><title>Rat Monoclonal Antibodies Differentiating between the Epstein-Barr Virus Nuclear Antigens 2A (EBNA2A) and 2B (EBNA2B)</title><author>Kremmer, Elisabeth ; 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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Antibody Specificity Epitope Mapping Epstein-Barr virus Epstein-Barr Virus Nuclear Antigens - immunology Hybridomas Rats Viral Proteins |
| title | Rat Monoclonal Antibodies Differentiating between the Epstein-Barr Virus Nuclear Antigens 2A (EBNA2A) and 2B (EBNA2B) |
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