Identification and Characterization of CEPU-Se—A Secreted Isoform of the IgLON Family Protein, CEPU-1
CEPU-1/Neurotrimin is a neuronal glycoprotein thought to play a role in axon guidance and cell–cell recognition. It is a member of the IgLON family, has three C2 domains, and is attached to the plasma membrane by a GPI-anchor. We report here the characterisation of an alternatively-spliced isoform o...
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| Veröffentlicht in: | Molecular and cellular neuroscience 2001-04, Vol.17 (4), p.746-760 |
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| creator | Lodge, Anthony P. McNamee, Christine J. Howard, Mark R. Reed, James E. Moss, Diana J. |
| description | CEPU-1/Neurotrimin is a neuronal glycoprotein thought to play a role in axon guidance and cell–cell recognition. It is a member of the IgLON family, has three C2 domains, and is attached to the plasma membrane by a GPI-anchor. We report here the characterisation of an alternatively-spliced isoform of CEPU-1 that is secreted. This isoform, termed CEPU-Se, is coexpressed with CEPU-1 in retina, cerebellum, and DRG neurons. In the cerebellum CEPU-1/CEPU-Se is expressed predominantly on granule cells and in the molecular layer. Divalent but not monovalent CEPU-Se interacts with CEPU-1 and other IgLONs, suggesting that the ability of CEPU-Se to modify the activity of the IgLON family may require an additional cofactor. CEPU-Se does not support the outgrowth of DRG neurons or the extension of established growth cones; however, neurite outgrowth on laminin is unaffected by CEPU-Se. Our data suggest that CEPU-Se may act to modulate the ability of CEPU-1, LAMP, and OBCAM to influence neurite outgrowth. |
| doi_str_mv | 10.1006/mcne.2001.0964 |
| format | Article |
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It is a member of the IgLON family, has three C2 domains, and is attached to the plasma membrane by a GPI-anchor. We report here the characterisation of an alternatively-spliced isoform of CEPU-1 that is secreted. This isoform, termed CEPU-Se, is coexpressed with CEPU-1 in retina, cerebellum, and DRG neurons. In the cerebellum CEPU-1/CEPU-Se is expressed predominantly on granule cells and in the molecular layer. Divalent but not monovalent CEPU-Se interacts with CEPU-1 and other IgLONs, suggesting that the ability of CEPU-Se to modify the activity of the IgLON family may require an additional cofactor. CEPU-Se does not support the outgrowth of DRG neurons or the extension of established growth cones; however, neurite outgrowth on laminin is unaffected by CEPU-Se. 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It is a member of the IgLON family, has three C2 domains, and is attached to the plasma membrane by a GPI-anchor. We report here the characterisation of an alternatively-spliced isoform of CEPU-1 that is secreted. This isoform, termed CEPU-Se, is coexpressed with CEPU-1 in retina, cerebellum, and DRG neurons. In the cerebellum CEPU-1/CEPU-Se is expressed predominantly on granule cells and in the molecular layer. Divalent but not monovalent CEPU-Se interacts with CEPU-1 and other IgLONs, suggesting that the ability of CEPU-Se to modify the activity of the IgLON family may require an additional cofactor. CEPU-Se does not support the outgrowth of DRG neurons or the extension of established growth cones; however, neurite outgrowth on laminin is unaffected by CEPU-Se. Our data suggest that CEPU-Se may act to modulate the ability of CEPU-1, LAMP, and OBCAM to influence neurite outgrowth.</description><subject>Alternative Splicing - physiology</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Avian Proteins</subject><subject>Base Sequence</subject><subject>Cell Division - physiology</subject><subject>CEPU-1 protein</subject><subject>Cerebellum - chemistry</subject><subject>Cerebellum - cytology</subject><subject>Cerebellum - physiology</subject><subject>Chick Embryo</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Dimerization</subject><subject>Ganglia, Spinal - cytology</subject><subject>Immunoglobulins - chemistry</subject><subject>Immunoglobulins - genetics</subject><subject>Isomerism</subject><subject>Membrane Glycoproteins - chemistry</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - secretion</subject><subject>Molecular Sequence Data</subject><subject>Neurites - physiology</subject><subject>Neurons, Afferent - cytology</subject><subject>neurotrimin</subject><subject>Protein Binding - physiology</subject><subject>Purkinje Cells - chemistry</subject><subject>Purkinje Cells - physiology</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Transfection</subject><issn>1044-7431</issn><issn>1095-9327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1OwzAUhS0E4n9lRJmYSPFPEscjqlqoVAESMFuufd0aNTHYLlKZeAiekCehUSoxIaZ7de93znAOQmcEDwjG1VWjWxhQjMkAi6rYQYcEizIXjPLdbi-KnBeMHKCjGF8wxiUVbB8dEMIIrbA4RPOJgTY567RKzreZak02XKigdILgPvqjt9lw9PCcP8L359d19gg6QAKTTaK3PjTdPy0gm8yn93fZWDVuuc4egk_g2steSU7QnlXLCKfbeYyex6On4W0-vb-ZDK-nuWacpFyQ2sxIbSkzABWpK15og1lti9KCqazhfAZUcKj4zGJlRakL0MYYUtCKU8uO0UXv-xr82wpiko2LGpZL1YJfRck55iXD4l-Q8LquS0Y34KAHdfAxBrDyNbhGhbUkWHYdyK4D2XUguw42gvOt82rWgPnFt6FvgLoHYBPEu4Mgo3bQajAugE7SePeX9w-CbpWB</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>Lodge, Anthony P.</creator><creator>McNamee, Christine J.</creator><creator>Howard, Mark R.</creator><creator>Reed, James E.</creator><creator>Moss, Diana J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20010401</creationdate><title>Identification and Characterization of CEPU-Se—A Secreted Isoform of the IgLON Family Protein, CEPU-1</title><author>Lodge, Anthony P. ; McNamee, Christine J. ; Howard, Mark R. ; Reed, James E. ; Moss, Diana J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-918db18f23dee618674cd038f45fed6fd77be297e67bf0af95c4ecddd142672f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Alternative Splicing - physiology</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Avian Proteins</topic><topic>Base Sequence</topic><topic>Cell Division - physiology</topic><topic>CEPU-1 protein</topic><topic>Cerebellum - chemistry</topic><topic>Cerebellum - cytology</topic><topic>Cerebellum - physiology</topic><topic>Chick Embryo</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Dimerization</topic><topic>Ganglia, Spinal - cytology</topic><topic>Immunoglobulins - chemistry</topic><topic>Immunoglobulins - genetics</topic><topic>Isomerism</topic><topic>Membrane Glycoproteins - chemistry</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - secretion</topic><topic>Molecular Sequence Data</topic><topic>Neurites - physiology</topic><topic>Neurons, Afferent - cytology</topic><topic>neurotrimin</topic><topic>Protein Binding - physiology</topic><topic>Purkinje Cells - chemistry</topic><topic>Purkinje Cells - physiology</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lodge, Anthony P.</creatorcontrib><creatorcontrib>McNamee, Christine J.</creatorcontrib><creatorcontrib>Howard, Mark R.</creatorcontrib><creatorcontrib>Reed, James E.</creatorcontrib><creatorcontrib>Moss, Diana J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lodge, Anthony P.</au><au>McNamee, Christine J.</au><au>Howard, Mark R.</au><au>Reed, James E.</au><au>Moss, Diana J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and Characterization of CEPU-Se—A Secreted Isoform of the IgLON Family Protein, CEPU-1</atitle><jtitle>Molecular and cellular neuroscience</jtitle><addtitle>Mol Cell Neurosci</addtitle><date>2001-04-01</date><risdate>2001</risdate><volume>17</volume><issue>4</issue><spage>746</spage><epage>760</epage><pages>746-760</pages><issn>1044-7431</issn><eissn>1095-9327</eissn><abstract>CEPU-1/Neurotrimin is a neuronal glycoprotein thought to play a role in axon guidance and cell–cell recognition. It is a member of the IgLON family, has three C2 domains, and is attached to the plasma membrane by a GPI-anchor. We report here the characterisation of an alternatively-spliced isoform of CEPU-1 that is secreted. This isoform, termed CEPU-Se, is coexpressed with CEPU-1 in retina, cerebellum, and DRG neurons. In the cerebellum CEPU-1/CEPU-Se is expressed predominantly on granule cells and in the molecular layer. Divalent but not monovalent CEPU-Se interacts with CEPU-1 and other IgLONs, suggesting that the ability of CEPU-Se to modify the activity of the IgLON family may require an additional cofactor. CEPU-Se does not support the outgrowth of DRG neurons or the extension of established growth cones; however, neurite outgrowth on laminin is unaffected by CEPU-Se. 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| subjects | Alternative Splicing - physiology Amino Acid Sequence Animals Avian Proteins Base Sequence Cell Division - physiology CEPU-1 protein Cerebellum - chemistry Cerebellum - cytology Cerebellum - physiology Chick Embryo CHO Cells Cricetinae Dimerization Ganglia, Spinal - cytology Immunoglobulins - chemistry Immunoglobulins - genetics Isomerism Membrane Glycoproteins - chemistry Membrane Glycoproteins - genetics Membrane Glycoproteins - secretion Molecular Sequence Data Neurites - physiology Neurons, Afferent - cytology neurotrimin Protein Binding - physiology Purkinje Cells - chemistry Purkinje Cells - physiology Recombinant Proteins - genetics Recombinant Proteins - metabolism Transfection |
| title | Identification and Characterization of CEPU-Se—A Secreted Isoform of the IgLON Family Protein, CEPU-1 |
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