Target-dependent differentiation and development of molluscan neurons and neuroendocrine cells: use of parasitisation as a tool

Specimens of the freshwater snail Lymnaea stagnalis infected with the schistosome parasite Trichobilharzia ocellata show a strongly inhibited development of their reproductive tract. We hypothesised that the effects of the underdevelopment of targets are reflected at the level of the neuronal develo...

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Veröffentlicht in:Neuroscience 2001-01, Vol.103 (1), p.289-299
Hauptverfasser: de Lange, R.P.J, Moorer-van Delft, C.M, de Boer, P.A.C.M, van Minnen, J, de Jong-Brink, M
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container_issue 1
container_start_page 289
container_title Neuroscience
container_volume 103
creator de Lange, R.P.J
Moorer-van Delft, C.M
de Boer, P.A.C.M
van Minnen, J
de Jong-Brink, M
description Specimens of the freshwater snail Lymnaea stagnalis infected with the schistosome parasite Trichobilharzia ocellata show a strongly inhibited development of their reproductive tract. We hypothesised that the effects of the underdevelopment of targets are reflected at the level of the neuronal development of (i) the motor neurons innervating the male copulation organ and (ii) neuroendocrine cells regulating the gonad. We determined the state of neuronal development by measuring cell number, cell size and neuropeptide gene expression. Our results show that the neuronal development of both copulation controlling anterior lobe motor neurons of the right cerebral ganglion and neuroendocrine caudodorsal cells, which produce neuropeptides regulating ovulation, egg laying and accompanying behaviour, are affected in parasitised animals in which their respective target organs were not developed. The cell bodies were smaller and fewer cells were found to express neuropeptide genes compared to those in non-parasitised animals. These effects were not observed in the appropriate controls. Backfills and lesions of the penis nerve have shown that the inhibited development of central motor neurons in parasitised snails is target dependent; neighbouring neurons that have no connection with the male copulation organ are not affected. Our data suggest that this effect is established by target-derived neurotrophic factors that need this connection for being transported to the innervating motor neurons. We propose that the effect on the neuroendocrine caudodorsal cells is mediated by a humoral factor, since they have no known connection with their target. We have shown that the size and gene expression of motor neurons controlling copulation behaviour in the pond snail Lymnaea stagnalis are related to the size of their target, the copulation organ, and depend on the connection with this target.
doi_str_mv 10.1016/S0306-4522(00)00556-X
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We hypothesised that the effects of the underdevelopment of targets are reflected at the level of the neuronal development of (i) the motor neurons innervating the male copulation organ and (ii) neuroendocrine cells regulating the gonad. We determined the state of neuronal development by measuring cell number, cell size and neuropeptide gene expression. Our results show that the neuronal development of both copulation controlling anterior lobe motor neurons of the right cerebral ganglion and neuroendocrine caudodorsal cells, which produce neuropeptides regulating ovulation, egg laying and accompanying behaviour, are affected in parasitised animals in which their respective target organs were not developed. The cell bodies were smaller and fewer cells were found to express neuropeptide genes compared to those in non-parasitised animals. These effects were not observed in the appropriate controls. Backfills and lesions of the penis nerve have shown that the inhibited development of central motor neurons in parasitised snails is target dependent; neighbouring neurons that have no connection with the male copulation organ are not affected. Our data suggest that this effect is established by target-derived neurotrophic factors that need this connection for being transported to the innervating motor neurons. We propose that the effect on the neuroendocrine caudodorsal cells is mediated by a humoral factor, since they have no known connection with their target. 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We hypothesised that the effects of the underdevelopment of targets are reflected at the level of the neuronal development of (i) the motor neurons innervating the male copulation organ and (ii) neuroendocrine cells regulating the gonad. We determined the state of neuronal development by measuring cell number, cell size and neuropeptide gene expression. Our results show that the neuronal development of both copulation controlling anterior lobe motor neurons of the right cerebral ganglion and neuroendocrine caudodorsal cells, which produce neuropeptides regulating ovulation, egg laying and accompanying behaviour, are affected in parasitised animals in which their respective target organs were not developed. The cell bodies were smaller and fewer cells were found to express neuropeptide genes compared to those in non-parasitised animals. These effects were not observed in the appropriate controls. 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Development</subject><subject>polyploidy</subject><subject>Schistosoma</subject><subject>schistosome parasites</subject><subject>snail brain</subject><subject>target organs</subject><subject>Trichobilharzia ocellata</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFrFTEUhYNY7LP6E5QBQXQxepNJMjPdiBS1hYILK3QX8pIbicwkYzJTcNW_bua9oS6bTS7kOyeXcwh5ReEDBSo__oAGZM0FY-8A3gMIIevbJ2RHu7apW8H5U7J7QE7J85x_QzmCN8_IKaUNpR30O3J_o9MvnGuLEwaLYa6sdw5TmbyefQyVDrayeIdDnMb1PbpqjMOwZKNDFXBJMeQDdJiLSTTJB6wMDkM-r5aMq2TSSWc_-7yZFkk1xzi8ICdODxlfbvcZ-fn1y83FZX39_dvVxefr2nAGc00t3Vst-z1w1NaAY446wSTrnWu7bm8kk7ZlKHkLLWjaOcu5oFwgFx0CNmfk7dF3SvHPgnlWo8_rijpgXLJqi47yRjwK0o7Kpu36AoojaFLMOaFTU_KjTn8VBbVWpA4VqTV_BaAOFanbonu9fbDsR7T_VVsnBXizAbpkPLikg_H5getBckYL9elIYUntzmNS2XgMBq1PaGZlo39kkX-nH6-e</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>de Lange, R.P.J</creator><creator>Moorer-van Delft, C.M</creator><creator>de Boer, P.A.C.M</creator><creator>van Minnen, J</creator><creator>de Jong-Brink, M</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20010101</creationdate><title>Target-dependent differentiation and development of molluscan neurons and neuroendocrine cells: use of parasitisation as a tool</title><author>de Lange, R.P.J ; Moorer-van Delft, C.M ; de Boer, P.A.C.M ; van Minnen, J ; de Jong-Brink, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-1d1bda69b04eadc0f2f1f52629ff788bc626d72e647070a18fd445145e458e0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Cell Differentiation</topic><topic>Cell Size</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gonads - innervation</topic><topic>Immunohistochemistry</topic><topic>Invertebrates</topic><topic>Life cycle. Embryology. Development</topic><topic>Lymnaea stagnalis</topic><topic>Male</topic><topic>Mollusca</topic><topic>Mollusca - parasitology</topic><topic>motor neurons</topic><topic>Motor Neurons - cytology</topic><topic>Motor Neurons - metabolism</topic><topic>neuroendocrine cells</topic><topic>Neuropeptide Y - metabolism</topic><topic>Neuropeptides - metabolism</topic><topic>Neurosecretory Systems - cytology</topic><topic>Physiology. 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We hypothesised that the effects of the underdevelopment of targets are reflected at the level of the neuronal development of (i) the motor neurons innervating the male copulation organ and (ii) neuroendocrine cells regulating the gonad. We determined the state of neuronal development by measuring cell number, cell size and neuropeptide gene expression. Our results show that the neuronal development of both copulation controlling anterior lobe motor neurons of the right cerebral ganglion and neuroendocrine caudodorsal cells, which produce neuropeptides regulating ovulation, egg laying and accompanying behaviour, are affected in parasitised animals in which their respective target organs were not developed. The cell bodies were smaller and fewer cells were found to express neuropeptide genes compared to those in non-parasitised animals. These effects were not observed in the appropriate controls. 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source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Animals
Biological and medical sciences
Cell Count
Cell Differentiation
Cell Size
Female
Fundamental and applied biological sciences. Psychology
Gonads - innervation
Immunohistochemistry
Invertebrates
Life cycle. Embryology. Development
Lymnaea stagnalis
Male
Mollusca
Mollusca - parasitology
motor neurons
Motor Neurons - cytology
Motor Neurons - metabolism
neuroendocrine cells
Neuropeptide Y - metabolism
Neuropeptides - metabolism
Neurosecretory Systems - cytology
Physiology. Development
polyploidy
Schistosoma
schistosome parasites
snail brain
target organs
Trichobilharzia ocellata
title Target-dependent differentiation and development of molluscan neurons and neuroendocrine cells: use of parasitisation as a tool
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