ATP-sensitive K+ transport in adrenal chromaffin granules
In the present study the influx of 86Rb+, a K+ analogue, was studied in mitochondria, microsomes and chromaffin granules prepared from adrenal gland medulla. The most active electrogenic 86Rb+ transport was found in the membrane fraction identified as chromaffin granules by marker enzyme estimation....
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| Veröffentlicht in: | Acta neurobiologiae experimentalis 2001-01, Vol.61 (1), p.1-12 |
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| container_title | Acta neurobiologiae experimentalis |
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| creator | Szewczyk, A Lobanov, N A Kicińska, A Wójcik, G Nałecz, M J |
| description | In the present study the influx of 86Rb+, a K+ analogue, was studied in mitochondria, microsomes and chromaffin granules prepared from adrenal gland medulla. The most active electrogenic 86Rb+ transport was found in the membrane fraction identified as chromaffin granules by marker enzyme estimation. The transport was found to be sensitive to ATP, ATP gamma S, ADP and to the triazine dyes, but not to AMP and cAMP. The inhibition induced by ATP was observed in the absence of externally added Mg2+, suggesting that a free nucleotide, rather than the ATP-Mg complex, was required for inhibition. Furthermore, the 86Rb+ influx was found to be inhibited by Mg2+ alone, but not by Ca2+ and antidiabetic sulfonylureas. The 86Rb+ influx was not stimulated by potassium channel openers. In conclusion, our results indicate that an electrogenic, ATP-sensitive potassium transport system operates in the chromaffin granule membrane. |
| doi_str_mv | 10.55782/ane-2001-1378 |
| format | Article |
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The most active electrogenic 86Rb+ transport was found in the membrane fraction identified as chromaffin granules by marker enzyme estimation. The transport was found to be sensitive to ATP, ATP gamma S, ADP and to the triazine dyes, but not to AMP and cAMP. The inhibition induced by ATP was observed in the absence of externally added Mg2+, suggesting that a free nucleotide, rather than the ATP-Mg complex, was required for inhibition. Furthermore, the 86Rb+ influx was found to be inhibited by Mg2+ alone, but not by Ca2+ and antidiabetic sulfonylureas. The 86Rb+ influx was not stimulated by potassium channel openers. 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In conclusion, our results indicate that an electrogenic, ATP-sensitive potassium transport system operates in the chromaffin granule membrane.</description><subject>Adenosine Triphosphate - physiology</subject><subject>Adrenal Glands - metabolism</subject><subject>Animals</subject><subject>Biological Transport, Active - drug effects</subject><subject>Biological Transport, Active - physiology</subject><subject>Cattle</subject><subject>Chromaffin Granules - drug effects</subject><subject>Chromaffin Granules - metabolism</subject><subject>Hypoglycemic Agents - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Magnesium - pharmacology</subject><subject>Potassium - metabolism</subject><issn>0065-1400</issn><issn>1689-0035</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1LxDAQxYMo7rp69Sg9eZGsM0nTpMdl8QsX9LCeQ5qmWunHmrSC_71Zt-BhGJh578H7EXKJsBRCKnZrOkcZAFLkUh2ROWYqpwBcHJM5QCYopgAzchbCJwCTksEpmSFyFByzOclX21caXBfqof52yfNNMnjThV3vh6TuElN615kmsR--b01VxdN7_I-NC-fkpDJNcBfTXpC3-7vt-pFuXh6e1qsNtSzHgRppCstAGqUgV4ClBMewqiymgrnSFUzYgqVZwfM48SQyWxmpuCqKLM0EX5DrQ-7O91-jC4Nu62Bd08Tm_Ri0lLFlLjEKlweh9X0I3lV65-vW-B-NoP9g6WjRe1h6DysarqbksWhd-S-f6PBfSERkMg</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Szewczyk, A</creator><creator>Lobanov, N A</creator><creator>Kicińska, A</creator><creator>Wójcik, G</creator><creator>Nałecz, M J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010101</creationdate><title>ATP-sensitive K+ transport in adrenal chromaffin granules</title><author>Szewczyk, A ; Lobanov, N A ; Kicińska, A ; Wójcik, G ; Nałecz, M J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c291t-a7abc207a8809801d70e21ffc1452edeb25cb246b396b352e56cfa7838bb64653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adenosine Triphosphate - physiology</topic><topic>Adrenal Glands - metabolism</topic><topic>Animals</topic><topic>Biological Transport, Active - drug effects</topic><topic>Biological Transport, Active - physiology</topic><topic>Cattle</topic><topic>Chromaffin Granules - drug effects</topic><topic>Chromaffin Granules - metabolism</topic><topic>Hypoglycemic Agents - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Magnesium - pharmacology</topic><topic>Potassium - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Szewczyk, A</creatorcontrib><creatorcontrib>Lobanov, N A</creatorcontrib><creatorcontrib>Kicińska, A</creatorcontrib><creatorcontrib>Wójcik, G</creatorcontrib><creatorcontrib>Nałecz, M J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta neurobiologiae experimentalis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Szewczyk, A</au><au>Lobanov, N A</au><au>Kicińska, A</au><au>Wójcik, G</au><au>Nałecz, M J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ATP-sensitive K+ transport in adrenal chromaffin granules</atitle><jtitle>Acta neurobiologiae experimentalis</jtitle><addtitle>Acta Neurobiol Exp (Wars)</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>61</volume><issue>1</issue><spage>1</spage><epage>12</epage><pages>1-12</pages><issn>0065-1400</issn><eissn>1689-0035</eissn><abstract>In the present study the influx of 86Rb+, a K+ analogue, was studied in mitochondria, microsomes and chromaffin granules prepared from adrenal gland medulla. 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| subjects | Adenosine Triphosphate - physiology Adrenal Glands - metabolism Animals Biological Transport, Active - drug effects Biological Transport, Active - physiology Cattle Chromaffin Granules - drug effects Chromaffin Granules - metabolism Hypoglycemic Agents - pharmacology In Vitro Techniques Magnesium - pharmacology Potassium - metabolism |
| title | ATP-sensitive K+ transport in adrenal chromaffin granules |
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