Effects of Opiates on High‐Affinity Ca2+,Mg2+‐ATPase in Brain Membrane Subfractions
The effect of a single administration of morphine sulfate (15 mg/kg, s.c. or 30 mg/kg, i.p., 30 min) on Ca2+‐stimulated Mg2+‐dependent ATPase activity was investigated in synaptosomal plasma membranes (SPM) prepared from rat cortex. Morphine produced a significant decrease in Ca2+,Mg2+‐ATPase activi...
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| Veröffentlicht in: | Journal of neurochemistry 1986-11, Vol.47 (5), p.1642-1646 |
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| container_title | Journal of neurochemistry |
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| creator | Pillai, N. P. Ross, D. H. |
| description | The effect of a single administration of morphine sulfate (15 mg/kg, s.c. or 30 mg/kg, i.p., 30 min) on Ca2+‐stimulated Mg2+‐dependent ATPase activity was investigated in synaptosomal plasma membranes (SPM) prepared from rat cortex. Morphine produced a significant decrease in Ca2+,Mg2+‐ATPase activity in synaptosomal fractions (SPM 1 + 2) known to contain a high density of opiate receptors and calmodulin‐dependent Ca2+,Mg2+‐ATPase. However, in another subpopulation (SPM 3) that contains fewer opiate receptors and less enzyme activity, no such decrease in the enzyme activity was observed after the opiate administration. The decrease in Ca2+,Mg2+‐ATPase activity seen in SPM 1 + 2 was specifically antagonized by the opiate antagonist naloxone hydrochloride (2 mg/kg, s.c.) when given 15 min before morphine administration. Mg2+‐ATP‐ase was not altered either by morphine or by a naloxone‐morphine combination. These findings give further evidence for the role of intracellular Ca2+ in mediating many of the acute effects of opiates. |
| doi_str_mv | 10.1111/j.1471-4159.1986.tb00807.x |
| format | Article |
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P. ; Ross, D. H.</creator><creatorcontrib>Pillai, N. P. ; Ross, D. H.</creatorcontrib><description>The effect of a single administration of morphine sulfate (15 mg/kg, s.c. or 30 mg/kg, i.p., 30 min) on Ca2+‐stimulated Mg2+‐dependent ATPase activity was investigated in synaptosomal plasma membranes (SPM) prepared from rat cortex. Morphine produced a significant decrease in Ca2+,Mg2+‐ATPase activity in synaptosomal fractions (SPM 1 + 2) known to contain a high density of opiate receptors and calmodulin‐dependent Ca2+,Mg2+‐ATPase. However, in another subpopulation (SPM 3) that contains fewer opiate receptors and less enzyme activity, no such decrease in the enzyme activity was observed after the opiate administration. The decrease in Ca2+,Mg2+‐ATPase activity seen in SPM 1 + 2 was specifically antagonized by the opiate antagonist naloxone hydrochloride (2 mg/kg, s.c.) when given 15 min before morphine administration. Mg2+‐ATP‐ase was not altered either by morphine or by a naloxone‐morphine combination. These findings give further evidence for the role of intracellular Ca2+ in mediating many of the acute effects of opiates.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1986.tb00807.x</identifier><identifier>PMID: 2944993</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Analgesics ; Animals ; Biological and medical sciences ; Brain - enzymology ; Brain - ultrastructure ; Ca(2+) Mg(2+)-ATPase - metabolism ; Ca2+,Mg2+‐ATPase ; Calcium-Transporting ATPases - metabolism ; Kinetics ; Medical sciences ; Membranes - enzymology ; Morphine ; Morphine - pharmacology ; Naloxone ; Naloxone - pharmacology ; Narcotics - pharmacology ; Neuropharmacology ; Pharmacology. Drug treatments ; Rats ; Synaptic plasma membrane</subject><ispartof>Journal of neurochemistry, 1986-11, Vol.47 (5), p.1642-1646</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1986.tb00807.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1986.tb00807.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8311787$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2944993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pillai, N. P.</creatorcontrib><creatorcontrib>Ross, D. H.</creatorcontrib><title>Effects of Opiates on High‐Affinity Ca2+,Mg2+‐ATPase in Brain Membrane Subfractions</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>The effect of a single administration of morphine sulfate (15 mg/kg, s.c. or 30 mg/kg, i.p., 30 min) on Ca2+‐stimulated Mg2+‐dependent ATPase activity was investigated in synaptosomal plasma membranes (SPM) prepared from rat cortex. Morphine produced a significant decrease in Ca2+,Mg2+‐ATPase activity in synaptosomal fractions (SPM 1 + 2) known to contain a high density of opiate receptors and calmodulin‐dependent Ca2+,Mg2+‐ATPase. However, in another subpopulation (SPM 3) that contains fewer opiate receptors and less enzyme activity, no such decrease in the enzyme activity was observed after the opiate administration. The decrease in Ca2+,Mg2+‐ATPase activity seen in SPM 1 + 2 was specifically antagonized by the opiate antagonist naloxone hydrochloride (2 mg/kg, s.c.) when given 15 min before morphine administration. Mg2+‐ATP‐ase was not altered either by morphine or by a naloxone‐morphine combination. These findings give further evidence for the role of intracellular Ca2+ in mediating many of the acute effects of opiates.</description><subject>Analgesics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - enzymology</subject><subject>Brain - ultrastructure</subject><subject>Ca(2+) Mg(2+)-ATPase - metabolism</subject><subject>Ca2+,Mg2+‐ATPase</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Kinetics</subject><subject>Medical sciences</subject><subject>Membranes - enzymology</subject><subject>Morphine</subject><subject>Morphine - pharmacology</subject><subject>Naloxone</subject><subject>Naloxone - pharmacology</subject><subject>Narcotics - pharmacology</subject><subject>Neuropharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Synaptic plasma membrane</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UctOwzAQtBAISuETkCKEuEDC2k78OCGogIJ4SYA4Wk5ig6s0LXEq6I1P4Bv5EhwRdQ-71s7IHs8gtI8hwaFOJglOOY5TnMkES8GSNgcQwJOvNTRYQetoAEBITCElW2jb-wkAZinDm2iTyDSVkg7Q64W1pmh9NLPRw9zp1oRjHY3d2_vv98-Zta527TIaaXJ0fPdGjrrl86P2JnJ1dN7o0O_MNG90baKnRW4bXbRuVvsdtGF15c1uP4fo5fLieTSObx-urkdnt_EcU0FiBtyUErhlTBhbYiOAQca4tKVI8xJTwrDNjc2gFBnRKc1lAUE6o5zKouB0iA7_7503s4-F8a2aOl-YqgqCZguvOIdMgsgCca8nLvKpKdW8cVPdLFXvRMAPelz7QlfhI3Xh_IomKMZcdO-d_tM-XWWWKxiD6pJRE9XZrzr7VZeM6pNRX-rmfhTcJ_QPwFmBxg</recordid><startdate>198611</startdate><enddate>198611</enddate><creator>Pillai, N. P.</creator><creator>Ross, D. H.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>198611</creationdate><title>Effects of Opiates on High‐Affinity Ca2+,Mg2+‐ATPase in Brain Membrane Subfractions</title><author>Pillai, N. P. ; Ross, D. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1382-607ed907f668efd1e80605679fd84bd13261fbef50d852a43b9c044963739cc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Analgesics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - enzymology</topic><topic>Brain - ultrastructure</topic><topic>Ca(2+) Mg(2+)-ATPase - metabolism</topic><topic>Ca2+,Mg2+‐ATPase</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Kinetics</topic><topic>Medical sciences</topic><topic>Membranes - enzymology</topic><topic>Morphine</topic><topic>Morphine - pharmacology</topic><topic>Naloxone</topic><topic>Naloxone - pharmacology</topic><topic>Narcotics - pharmacology</topic><topic>Neuropharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Rats</topic><topic>Synaptic plasma membrane</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pillai, N. P.</creatorcontrib><creatorcontrib>Ross, D. H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pillai, N. P.</au><au>Ross, D. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Opiates on High‐Affinity Ca2+,Mg2+‐ATPase in Brain Membrane Subfractions</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1986-11</date><risdate>1986</risdate><volume>47</volume><issue>5</issue><spage>1642</spage><epage>1646</epage><pages>1642-1646</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>The effect of a single administration of morphine sulfate (15 mg/kg, s.c. or 30 mg/kg, i.p., 30 min) on Ca2+‐stimulated Mg2+‐dependent ATPase activity was investigated in synaptosomal plasma membranes (SPM) prepared from rat cortex. Morphine produced a significant decrease in Ca2+,Mg2+‐ATPase activity in synaptosomal fractions (SPM 1 + 2) known to contain a high density of opiate receptors and calmodulin‐dependent Ca2+,Mg2+‐ATPase. However, in another subpopulation (SPM 3) that contains fewer opiate receptors and less enzyme activity, no such decrease in the enzyme activity was observed after the opiate administration. The decrease in Ca2+,Mg2+‐ATPase activity seen in SPM 1 + 2 was specifically antagonized by the opiate antagonist naloxone hydrochloride (2 mg/kg, s.c.) when given 15 min before morphine administration. Mg2+‐ATP‐ase was not altered either by morphine or by a naloxone‐morphine combination. These findings give further evidence for the role of intracellular Ca2+ in mediating many of the acute effects of opiates.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>2944993</pmid><doi>10.1111/j.1471-4159.1986.tb00807.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of neurochemistry, 1986-11, Vol.47 (5), p.1642-1646 |
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| subjects | Analgesics Animals Biological and medical sciences Brain - enzymology Brain - ultrastructure Ca(2+) Mg(2+)-ATPase - metabolism Ca2+,Mg2+‐ATPase Calcium-Transporting ATPases - metabolism Kinetics Medical sciences Membranes - enzymology Morphine Morphine - pharmacology Naloxone Naloxone - pharmacology Narcotics - pharmacology Neuropharmacology Pharmacology. Drug treatments Rats Synaptic plasma membrane |
| title | Effects of Opiates on High‐Affinity Ca2+,Mg2+‐ATPase in Brain Membrane Subfractions |
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