Uridine phosphorylase association with vimentin. Intracellular distribution and localization

Uridine phosphorylase (UPase), a key enzyme in the pyrimidine salvage pathway, is associated with the intermediate filament protein vimentin, in NIH 3T3 fibroblasts and colon 26 cells. Affinity chromatography was utilized to purify UPase from colon 26 and NIH 3T3 cells using the uridine phosphorylas...

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Veröffentlicht in:	The Journal of biological chemistry 2001-04, Vol.276 (16), p.13302-13307
Hauptverfasser:	Russell, R L, Cao, D, Zhang, D, Handschumacher, R E, Pizzorno, G
Format:	Artikel
Sprache:	eng
Schlagworte:	3T3 Cells Animals Chromatography, Affinity Colon Cytoskeleton - ultrastructure Fluorescent Antibody Technique Humans Kinetics Mice Peptide Fragments - chemistry Protein Binding Recombinant Proteins - analysis Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Uridine Phosphorylase - analysis Uridine Phosphorylase - isolation & purification Uridine Phosphorylase - metabolism Vimentin - analysis Vimentin - isolation & purification Vimentin - metabolism
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