Construction of 700 human/mouse A9 monochromosomal hybrids and analysis of imprinted genes on human chromosome 6
As an in vitro assay system for the identification of human imprinted genes, a library of human/mouse A9 monochromosomal hybrids containing a single, intact bsr-tagged human chromosome of known parental origin, derived from normal human fibroblasts, has been previously generated by microcell-mediate...
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| Veröffentlicht in: | Journal of human genetics 2001-01, Vol.46 (3), p.137-145 |
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| creator | Inoue, J. Mitsuya, K. Maegawa, S. Kugoh, H. Kadota, M. Okamura, D. Shinohara, T. Nishihara, S. Takehara, S. Yamauchi, K. Schulz, T. C. Oshimura, M. |
| description | As an in vitro assay system for the identification of human imprinted genes, a library of human/mouse A9 monochromosomal hybrids containing a single, intact bsr-tagged human chromosome of known parental origin, derived from normal human fibroblasts, has been previously generated by microcell-mediated chromosome transfer (MMCT). To supplement this assay system, we constructed additional 700 A9 monochromosomal hybrids, using a pST
neo
or pPGK
neo
selection marker. To validate the A9 hybrids, we screened them with chromosome-specific polymorphic markers, and identified the hybrids containing either human chromosome 6, 7, 14, 18, or 21 of known parental origin. Matching paternal and maternal chromosome pairs of A9 hybrids were identified for chromosomes 6, 7, 14, and 18. The paternal-specific expression of ZAC (zinc finger protein, which regulates apoptosis and cell cycle arrest) and HYMAI (hydatidiform mole-associated and imprinted transcript), and the maternal-specific methylation of a CpG island within an imprinted domain on human chromosome 6q24, were maintained in A9 hybrids. For an example, we profiled the expression of expressed sequence tags (ESTs) and the methylation of CpG islands in the 300-kb imprinted domain around 6q24, which may be associated with cancers and transient neonatal diabetes mellitus (TNDM). Thus, the 700 A9 hybrids should be useful for various aspects of imprinting studies. |
| doi_str_mv | 10.1007/s100380170101 |
| format | Article |
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neo
or pPGK
neo
selection marker. To validate the A9 hybrids, we screened them with chromosome-specific polymorphic markers, and identified the hybrids containing either human chromosome 6, 7, 14, 18, or 21 of known parental origin. Matching paternal and maternal chromosome pairs of A9 hybrids were identified for chromosomes 6, 7, 14, and 18. The paternal-specific expression of ZAC (zinc finger protein, which regulates apoptosis and cell cycle arrest) and HYMAI (hydatidiform mole-associated and imprinted transcript), and the maternal-specific methylation of a CpG island within an imprinted domain on human chromosome 6q24, were maintained in A9 hybrids. For an example, we profiled the expression of expressed sequence tags (ESTs) and the methylation of CpG islands in the 300-kb imprinted domain around 6q24, which may be associated with cancers and transient neonatal diabetes mellitus (TNDM). Thus, the 700 A9 hybrids should be useful for various aspects of imprinting studies.</description><identifier>ISSN: 1434-5161</identifier><identifier>EISSN: 1435-232X</identifier><identifier>DOI: 10.1007/s100380170101</identifier><identifier>PMID: 11310581</identifier><language>eng</language><publisher>Tokyo: Springer-Verlag</publisher><subject>Animals ; Apoptosis ; Base Sequence ; Biomedical and Life Sciences ; Biomedicine ; Cell cycle ; Chromosome 6 ; Chromosomes ; Chromosomes, Human, Pair 6 - genetics ; CpG Islands ; Diabetes mellitus ; DNA Methylation ; DNA Primers - genetics ; Expressed Sequence Tags ; Female ; Fibroblasts ; Gene Expression ; Gene Function ; Gene Therapy ; Genetic Techniques ; Genomic Imprinting ; Human Genetics ; Humans ; Hybrid Cells ; Hybrids ; Hydatidiform mole ; In Situ Hybridization, Fluorescence ; Infant, Newborn ; Male ; Mice ; Molecular Medicine ; Neonates ; Original Article ; Transcription ; Zinc finger proteins</subject><ispartof>Journal of human genetics, 2001-01, Vol.46 (3), p.137-145</ispartof><rights>The Japanese Society of Human Genetics and Springer-Verlag Tokyo 2001</rights><rights>The Japanese Society of Human Genetics and Springer-Verlag Tokyo 2001.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-347360edf4fc5b6748031756c6e0aa0b1b76787ec8c99669047eb16ac5357da03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s100380170101$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s100380170101$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11310581$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inoue, J.</creatorcontrib><creatorcontrib>Mitsuya, K.</creatorcontrib><creatorcontrib>Maegawa, S.</creatorcontrib><creatorcontrib>Kugoh, H.</creatorcontrib><creatorcontrib>Kadota, M.</creatorcontrib><creatorcontrib>Okamura, D.</creatorcontrib><creatorcontrib>Shinohara, T.</creatorcontrib><creatorcontrib>Nishihara, S.</creatorcontrib><creatorcontrib>Takehara, S.</creatorcontrib><creatorcontrib>Yamauchi, K.</creatorcontrib><creatorcontrib>Schulz, T. C.</creatorcontrib><creatorcontrib>Oshimura, M.</creatorcontrib><title>Construction of 700 human/mouse A9 monochromosomal hybrids and analysis of imprinted genes on human chromosome 6</title><title>Journal of human genetics</title><addtitle>J Hum Genet</addtitle><addtitle>J Hum Genet</addtitle><description>As an in vitro assay system for the identification of human imprinted genes, a library of human/mouse A9 monochromosomal hybrids containing a single, intact bsr-tagged human chromosome of known parental origin, derived from normal human fibroblasts, has been previously generated by microcell-mediated chromosome transfer (MMCT). To supplement this assay system, we constructed additional 700 A9 monochromosomal hybrids, using a pST
neo
or pPGK
neo
selection marker. To validate the A9 hybrids, we screened them with chromosome-specific polymorphic markers, and identified the hybrids containing either human chromosome 6, 7, 14, 18, or 21 of known parental origin. Matching paternal and maternal chromosome pairs of A9 hybrids were identified for chromosomes 6, 7, 14, and 18. The paternal-specific expression of ZAC (zinc finger protein, which regulates apoptosis and cell cycle arrest) and HYMAI (hydatidiform mole-associated and imprinted transcript), and the maternal-specific methylation of a CpG island within an imprinted domain on human chromosome 6q24, were maintained in A9 hybrids. For an example, we profiled the expression of expressed sequence tags (ESTs) and the methylation of CpG islands in the 300-kb imprinted domain around 6q24, which may be associated with cancers and transient neonatal diabetes mellitus (TNDM). Thus, the 700 A9 hybrids should be useful for various aspects of imprinting studies.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Base Sequence</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell cycle</subject><subject>Chromosome 6</subject><subject>Chromosomes</subject><subject>Chromosomes, Human, Pair 6 - genetics</subject><subject>CpG Islands</subject><subject>Diabetes mellitus</subject><subject>DNA Methylation</subject><subject>DNA Primers - genetics</subject><subject>Expressed Sequence Tags</subject><subject>Female</subject><subject>Fibroblasts</subject><subject>Gene Expression</subject><subject>Gene Function</subject><subject>Gene Therapy</subject><subject>Genetic Techniques</subject><subject>Genomic Imprinting</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Hybrid Cells</subject><subject>Hybrids</subject><subject>Hydatidiform mole</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Medicine</subject><subject>Neonates</subject><subject>Original Article</subject><subject>Transcription</subject><subject>Zinc finger proteins</subject><issn>1434-5161</issn><issn>1435-232X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kc1r3DAQxUVJab567LURFHJzMiNZkvcYluYDArkkkJuRZXnXiyVtJfuw_3209ZLQQg4aieH3nngzhPxAuEIAdZ1y5RWgAgT8Qk6w5KJgnL0e_X2XhUCJx-Q0pQ1kkin2jRwjcgRR4QnZLoNPY5zM2AdPQ0cVAF1PTvtrF6Zk6c2CuuCDWcfgQgpOD3S9a2LfJqp9m48edqlPe2nvtrH3o23pynqbW352ou9iS-U5-drpIdnvh_uMvNz-fl7eF49Pdw_Lm8fCCC7HgpeKS7BtV3ZGNFKVFXBUQhppQWtosFFSVcqayiwWUi6gVLZBqbNaqFYDPyOXs-82hj-TTWPt-mTsMGhvc7BaKRBS4R789R-4CVPMsVLNSiYklopVmSpmysSQUrRdnbM6HXc1Qr1fRP3PIjL_8-A6Nc62H_Rh8hm4moG0H9rKxo9vP3O8mAVej1O0746b9YoBIFP8DSt4mxE</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Inoue, J.</creator><creator>Mitsuya, K.</creator><creator>Maegawa, S.</creator><creator>Kugoh, H.</creator><creator>Kadota, M.</creator><creator>Okamura, D.</creator><creator>Shinohara, T.</creator><creator>Nishihara, S.</creator><creator>Takehara, S.</creator><creator>Yamauchi, K.</creator><creator>Schulz, T. C.</creator><creator>Oshimura, M.</creator><general>Springer-Verlag</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20010101</creationdate><title>Construction of 700 human/mouse A9 monochromosomal hybrids and analysis of imprinted genes on human chromosome 6</title><author>Inoue, J. ; Mitsuya, K. ; Maegawa, S. ; Kugoh, H. ; Kadota, M. ; Okamura, D. ; Shinohara, T. ; Nishihara, S. ; Takehara, S. ; Yamauchi, K. ; Schulz, T. 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C.</au><au>Oshimura, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of 700 human/mouse A9 monochromosomal hybrids and analysis of imprinted genes on human chromosome 6</atitle><jtitle>Journal of human genetics</jtitle><stitle>J Hum Genet</stitle><addtitle>J Hum Genet</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>46</volume><issue>3</issue><spage>137</spage><epage>145</epage><pages>137-145</pages><issn>1434-5161</issn><eissn>1435-232X</eissn><abstract>As an in vitro assay system for the identification of human imprinted genes, a library of human/mouse A9 monochromosomal hybrids containing a single, intact bsr-tagged human chromosome of known parental origin, derived from normal human fibroblasts, has been previously generated by microcell-mediated chromosome transfer (MMCT). To supplement this assay system, we constructed additional 700 A9 monochromosomal hybrids, using a pST
neo
or pPGK
neo
selection marker. To validate the A9 hybrids, we screened them with chromosome-specific polymorphic markers, and identified the hybrids containing either human chromosome 6, 7, 14, 18, or 21 of known parental origin. Matching paternal and maternal chromosome pairs of A9 hybrids were identified for chromosomes 6, 7, 14, and 18. The paternal-specific expression of ZAC (zinc finger protein, which regulates apoptosis and cell cycle arrest) and HYMAI (hydatidiform mole-associated and imprinted transcript), and the maternal-specific methylation of a CpG island within an imprinted domain on human chromosome 6q24, were maintained in A9 hybrids. For an example, we profiled the expression of expressed sequence tags (ESTs) and the methylation of CpG islands in the 300-kb imprinted domain around 6q24, which may be associated with cancers and transient neonatal diabetes mellitus (TNDM). Thus, the 700 A9 hybrids should be useful for various aspects of imprinting studies.</abstract><cop>Tokyo</cop><pub>Springer-Verlag</pub><pmid>11310581</pmid><doi>10.1007/s100380170101</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis Base Sequence Biomedical and Life Sciences Biomedicine Cell cycle Chromosome 6 Chromosomes Chromosomes, Human, Pair 6 - genetics CpG Islands Diabetes mellitus DNA Methylation DNA Primers - genetics Expressed Sequence Tags Female Fibroblasts Gene Expression Gene Function Gene Therapy Genetic Techniques Genomic Imprinting Human Genetics Humans Hybrid Cells Hybrids Hydatidiform mole In Situ Hybridization, Fluorescence Infant, Newborn Male Mice Molecular Medicine Neonates Original Article Transcription Zinc finger proteins |
| title | Construction of 700 human/mouse A9 monochromosomal hybrids and analysis of imprinted genes on human chromosome 6 |
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