Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum
The genes for the two subunits of the formate dehydrogenase from Methanobacterium formicicum were cloned and their sequences determined. When expressed in Escherichia coli, two proteins were produced which had the appropriate mobility on an SDS gel for the two subunits of formate dehydrogenase and c...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 1986-10, Vol.261 (28), p.12942-12947 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 12947 |
|---|---|
| container_issue | 28 |
| container_start_page | 12942 |
| container_title | The Journal of biological chemistry |
| container_volume | 261 |
| creator | Shuber, A P Orr, E C Recny, M A Schendel, P F May, H D Schauer, N L Ferry, J G |
| description | The genes for the two subunits of the formate dehydrogenase from Methanobacterium formicicum were cloned and their sequences determined. When expressed in Escherichia coli, two proteins were produced which had the appropriate mobility on an SDS gel for the two subunits of formate dehydrogenase and cross-reacted with antibodies raised to purified formate dehydrogenase. The genes for the two formate dehydrogenase subunits overlap by 1 base pair and are preceded by DNA sequences similar to both eubacterial and archaebacterial promoters and ribosome-binding sites. The amino acid sequences deduced from the DNA sequence were analyzed, and the arrangement of putative iron-sulfur centers is discussed. |
| doi_str_mv | 10.1016/S0021-9258(18)69253-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77054608</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021925818692531</els_id><sourcerecordid>77054608</sourcerecordid><originalsourceid>FETCH-LOGICAL-c465t-d18eee0bd7c7aa8b7e380f0dd59d9a1124a757d37a91bafd17cf30ca902cf76d3</originalsourceid><addsrcrecordid>eNqFkV2L1DAUhoO4rOPoT1jIhYjCVnOatmmvRAY_Fla8UMG7kCYn00ibjEmru__ezAfj5ebmBM7z5hyeEHIF7A0waN5-Y6yEoivr9hW0r5t84QU8IitgLS94DT8fk9UZeUKepvSL5VN1cEkuec0BumpFdpsxeOe31xTvdhFTcsFfU-UN9YseMczOIE34e0GvkQZL5wGpDXFSM1KDw72JYYteJaS5YKI2hol-wXlQPvRKzxjdMh0STju9TM_IhVVjwuenuiY_Pn74vvlc3H79dLN5f1voqqnnwkCLiKw3Qgul2l4gb5llxtSd6RRAWSlRC8OF6qBX1oDQljOtOlZqKxrD1-Tl8d1dDHn7NMvJJY3jqDyGJUkhWF012dWa1EdQx5BSRCt30U0q3ktgcm9aHkzLvUYJrTyYlpBzV6cBSz-hOadOanP_xamvklajjcprl85Ym18WrPuPDW47_HURZe-CHnCSZQOyzCPLrioz9u6IYXb2x2GUSbv9n5gc0bM0wT2w7z8faakM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77054608</pqid></control><display><type>article</type><title>Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Shuber, A P ; Orr, E C ; Recny, M A ; Schendel, P F ; May, H D ; Schauer, N L ; Ferry, J G</creator><creatorcontrib>Shuber, A P ; Orr, E C ; Recny, M A ; Schendel, P F ; May, H D ; Schauer, N L ; Ferry, J G</creatorcontrib><description>The genes for the two subunits of the formate dehydrogenase from Methanobacterium formicicum were cloned and their sequences determined. When expressed in Escherichia coli, two proteins were produced which had the appropriate mobility on an SDS gel for the two subunits of formate dehydrogenase and cross-reacted with antibodies raised to purified formate dehydrogenase. The genes for the two formate dehydrogenase subunits overlap by 1 base pair and are preceded by DNA sequences similar to both eubacterial and archaebacterial promoters and ribosome-binding sites. The amino acid sequences deduced from the DNA sequence were analyzed, and the arrangement of putative iron-sulfur centers is discussed.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)69253-1</identifier><identifier>PMID: 3531194</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Aldehyde Oxidoreductases - genetics ; Amino Acid Sequence ; Antibodies ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Cloning, Molecular ; Cross Reactions ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli - genetics ; Euryarchaeota - enzymology ; Euryarchaeota - genetics ; Formate Dehydrogenases - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Genetics ; Macromolecular Substances ; Microbiology ; Mission oriented research</subject><ispartof>The Journal of biological chemistry, 1986-10, Vol.261 (28), p.12942-12947</ispartof><rights>1986 © 1986 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-d18eee0bd7c7aa8b7e380f0dd59d9a1124a757d37a91bafd17cf30ca902cf76d3</citedby><cites>FETCH-LOGICAL-c465t-d18eee0bd7c7aa8b7e380f0dd59d9a1124a757d37a91bafd17cf30ca902cf76d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8016709$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3531194$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shuber, A P</creatorcontrib><creatorcontrib>Orr, E C</creatorcontrib><creatorcontrib>Recny, M A</creatorcontrib><creatorcontrib>Schendel, P F</creatorcontrib><creatorcontrib>May, H D</creatorcontrib><creatorcontrib>Schauer, N L</creatorcontrib><creatorcontrib>Ferry, J G</creatorcontrib><title>Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The genes for the two subunits of the formate dehydrogenase from Methanobacterium formicicum were cloned and their sequences determined. When expressed in Escherichia coli, two proteins were produced which had the appropriate mobility on an SDS gel for the two subunits of formate dehydrogenase and cross-reacted with antibodies raised to purified formate dehydrogenase. The genes for the two formate dehydrogenase subunits overlap by 1 base pair and are preceded by DNA sequences similar to both eubacterial and archaebacterial promoters and ribosome-binding sites. The amino acid sequences deduced from the DNA sequence were analyzed, and the arrangement of putative iron-sulfur centers is discussed.</description><subject>Aldehyde Oxidoreductases - genetics</subject><subject>Amino Acid Sequence</subject><subject>Antibodies</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>Cross Reactions</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Escherichia coli - genetics</subject><subject>Euryarchaeota - enzymology</subject><subject>Euryarchaeota - genetics</subject><subject>Formate Dehydrogenases - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Genetics</subject><subject>Macromolecular Substances</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2L1DAUhoO4rOPoT1jIhYjCVnOatmmvRAY_Fla8UMG7kCYn00ibjEmru__ezAfj5ebmBM7z5hyeEHIF7A0waN5-Y6yEoivr9hW0r5t84QU8IitgLS94DT8fk9UZeUKepvSL5VN1cEkuec0BumpFdpsxeOe31xTvdhFTcsFfU-UN9YseMczOIE34e0GvkQZL5wGpDXFSM1KDw72JYYteJaS5YKI2hol-wXlQPvRKzxjdMh0STju9TM_IhVVjwuenuiY_Pn74vvlc3H79dLN5f1voqqnnwkCLiKw3Qgul2l4gb5llxtSd6RRAWSlRC8OF6qBX1oDQljOtOlZqKxrD1-Tl8d1dDHn7NMvJJY3jqDyGJUkhWF012dWa1EdQx5BSRCt30U0q3ktgcm9aHkzLvUYJrTyYlpBzV6cBSz-hOadOanP_xamvklajjcprl85Ym18WrPuPDW47_HURZe-CHnCSZQOyzCPLrioz9u6IYXb2x2GUSbv9n5gc0bM0wT2w7z8faakM</recordid><startdate>19861005</startdate><enddate>19861005</enddate><creator>Shuber, A P</creator><creator>Orr, E C</creator><creator>Recny, M A</creator><creator>Schendel, P F</creator><creator>May, H D</creator><creator>Schauer, N L</creator><creator>Ferry, J G</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19861005</creationdate><title>Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum</title><author>Shuber, A P ; Orr, E C ; Recny, M A ; Schendel, P F ; May, H D ; Schauer, N L ; Ferry, J G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-d18eee0bd7c7aa8b7e380f0dd59d9a1124a757d37a91bafd17cf30ca902cf76d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Aldehyde Oxidoreductases - genetics</topic><topic>Amino Acid Sequence</topic><topic>Antibodies</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>Cross Reactions</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Escherichia coli - genetics</topic><topic>Euryarchaeota - enzymology</topic><topic>Euryarchaeota - genetics</topic><topic>Formate Dehydrogenases - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Genetics</topic><topic>Macromolecular Substances</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shuber, A P</creatorcontrib><creatorcontrib>Orr, E C</creatorcontrib><creatorcontrib>Recny, M A</creatorcontrib><creatorcontrib>Schendel, P F</creatorcontrib><creatorcontrib>May, H D</creatorcontrib><creatorcontrib>Schauer, N L</creatorcontrib><creatorcontrib>Ferry, J G</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shuber, A P</au><au>Orr, E C</au><au>Recny, M A</au><au>Schendel, P F</au><au>May, H D</au><au>Schauer, N L</au><au>Ferry, J G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1986-10-05</date><risdate>1986</risdate><volume>261</volume><issue>28</issue><spage>12942</spage><epage>12947</epage><pages>12942-12947</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>The genes for the two subunits of the formate dehydrogenase from Methanobacterium formicicum were cloned and their sequences determined. When expressed in Escherichia coli, two proteins were produced which had the appropriate mobility on an SDS gel for the two subunits of formate dehydrogenase and cross-reacted with antibodies raised to purified formate dehydrogenase. The genes for the two formate dehydrogenase subunits overlap by 1 base pair and are preceded by DNA sequences similar to both eubacterial and archaebacterial promoters and ribosome-binding sites. The amino acid sequences deduced from the DNA sequence were analyzed, and the arrangement of putative iron-sulfur centers is discussed.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>3531194</pmid><doi>10.1016/S0021-9258(18)69253-1</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1986-10, Vol.261 (28), p.12942-12947 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77054608 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Aldehyde Oxidoreductases - genetics Amino Acid Sequence Antibodies Bacteriology Base Sequence Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Cloning, Molecular Cross Reactions Enzyme-Linked Immunosorbent Assay Escherichia coli - genetics Euryarchaeota - enzymology Euryarchaeota - genetics Formate Dehydrogenases - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genetics Macromolecular Substances Microbiology Mission oriented research |
| title | Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T01%3A46%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning,%20expression,%20and%20nucleotide%20sequence%20of%20the%20formate%20dehydrogenase%20genes%20from%20Methanobacterium%20formicicum&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Shuber,%20A%20P&rft.date=1986-10-05&rft.volume=261&rft.issue=28&rft.spage=12942&rft.epage=12947&rft.pages=12942-12947&rft.issn=0021-9258&rft.eissn=1083-351X&rft.coden=JBCHA3&rft_id=info:doi/10.1016/S0021-9258(18)69253-1&rft_dat=%3Cproquest_cross%3E77054608%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77054608&rft_id=info:pmid/3531194&rft_els_id=S0021925818692531&rfr_iscdi=true |