A Sesquiterpene Lactone, Costunolide, from Magnolia grandiflora Inhibits NF-κB by Targeting IκB Phosphorylation

Abstract A sesquiterpene lactone, costunolide (CTN), was identified from MAGNOLIA GRANDIFLORA together with parthenolide (PTN) by its strong inhibition of LPS-induced NF-κB activation. CTN, which showed more potent inhibition than PTN in the NF-κB activation, strongly suppressed nitric oxide (NO) pr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Planta medica 2001-03, Vol.67 (2), p.103-107
Hauptverfasser: Koo, Tae Hyeon, Lee, Jeong-Hyung, Park, Yun Joo, Hong, Young-Soo, Kim, Hang Sub, Kim, Kyu-Won, Lee, Jung Joon
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 107
container_issue 2
container_start_page 103
container_title Planta medica
container_volume 67
creator Koo, Tae Hyeon
Lee, Jeong-Hyung
Park, Yun Joo
Hong, Young-Soo
Kim, Hang Sub
Kim, Kyu-Won
Lee, Jung Joon
description Abstract A sesquiterpene lactone, costunolide (CTN), was identified from MAGNOLIA GRANDIFLORA together with parthenolide (PTN) by its strong inhibition of LPS-induced NF-κB activation. CTN, which showed more potent inhibition than PTN in the NF-κB activation, strongly suppressed nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. RT-PCR and Western blot analyses demonstrated that CTN suppressed the expression of iNOS mRNA and protein in a dose-dependent manner. CTN also significantly inhibited LPS-induced DNA-binding activity of NF-κB as well as the LPS-induced degradation of IκB-α and -β. Furthermore, CTN inhibited LPS-induced phosphorylation of IκB-α. These findings support that CTN inhibits NO production by down-regulating iNOS expression, at least, in part through the inhibition of IκBs' phosphorylation and degradation, which are essential for the activation of NF-κB.
doi_str_mv 10.1055/s-2001-11503
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77053946</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>77053946</sourcerecordid><originalsourceid>FETCH-LOGICAL-c351t-b1baebac5195cebb99eea041647a78469a208994144a085e24db43f3f48697d43</originalsourceid><addsrcrecordid>eNpt0M1uEzEQwHELgWgo3DgjS0hc6BZ_ZtfHNqIQKQUkytma3Z1NXO3aie095NX6EDxTN01ULpyssX6akf6EvOfskjOtv6RCMMYLzjWTL8iMK2kKJgR_SWaMSVEwo-QZeZPS_cSUYew1OeNcMl5pMSO7K_ob0250GeMWPdIVNDl4vKCLkPLoQ-_aaehiGOgtrA8z0HUE37quDxHo0m9c7XKiP26Kvw_XtN7TO4hrzM6v6fLw82sT0nYT4r6H7IJ_S1510Cd8d3rPyZ-br3eL78Xq57fl4mpVNFLzXNS8Bqyh0dzoBuvaGERgis9VCWWl5gYEq4xRXClglUah2lrJTnaqmpuyVfKcfDru3cawGzFlO7jUYN-DxzAmW5ZMS6PmE7w4wiaGlCJ2dhvdAHFvObOHxDbZQ2L7lHjiH057x3rA9h8-NZ3AxxOA1EDfTbEal56dEVwoM6nPR5U3Dge092GMfurx_6OPJYWSAQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77053946</pqid></control><display><type>article</type><title>A Sesquiterpene Lactone, Costunolide, from Magnolia grandiflora Inhibits NF-κB by Targeting IκB Phosphorylation</title><source>MEDLINE</source><source>Thieme Connect Journals</source><creator>Koo, Tae Hyeon ; Lee, Jeong-Hyung ; Park, Yun Joo ; Hong, Young-Soo ; Kim, Hang Sub ; Kim, Kyu-Won ; Lee, Jung Joon</creator><creatorcontrib>Koo, Tae Hyeon ; Lee, Jeong-Hyung ; Park, Yun Joo ; Hong, Young-Soo ; Kim, Hang Sub ; Kim, Kyu-Won ; Lee, Jung Joon</creatorcontrib><description>Abstract A sesquiterpene lactone, costunolide (CTN), was identified from MAGNOLIA GRANDIFLORA together with parthenolide (PTN) by its strong inhibition of LPS-induced NF-κB activation. CTN, which showed more potent inhibition than PTN in the NF-κB activation, strongly suppressed nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. RT-PCR and Western blot analyses demonstrated that CTN suppressed the expression of iNOS mRNA and protein in a dose-dependent manner. CTN also significantly inhibited LPS-induced DNA-binding activity of NF-κB as well as the LPS-induced degradation of IκB-α and -β. Furthermore, CTN inhibited LPS-induced phosphorylation of IκB-α. These findings support that CTN inhibits NO production by down-regulating iNOS expression, at least, in part through the inhibition of IκBs' phosphorylation and degradation, which are essential for the activation of NF-κB.</description><identifier>ISSN: 0032-0943</identifier><identifier>EISSN: 1439-0221</identifier><identifier>DOI: 10.1055/s-2001-11503</identifier><identifier>PMID: 11301852</identifier><identifier>CODEN: PLMEAA</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>Animals ; Biological and medical sciences ; Cell Line - drug effects ; Dose-Response Relationship, Drug ; Gene Expression Regulation - drug effects ; General pharmacology ; I-kappa B Proteins - metabolism ; Lipopolysaccharides - metabolism ; Macrophages - drug effects ; Medical sciences ; Mice ; Molecular Structure ; NF-kappa B - antagonists &amp; inhibitors ; NF-kappa B - metabolism ; Nitric Oxide - antagonists &amp; inhibitors ; Nitric Oxide - metabolism ; Nitric Oxide Synthase - metabolism ; Nitric Oxide Synthase Type II ; Original Paper ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Phosphorylation ; Plant Leaves - chemistry ; Plants, Medicinal - chemistry ; Sesquiterpenes - isolation &amp; purification ; Sesquiterpenes - pharmacology</subject><ispartof>Planta medica, 2001-03, Vol.67 (2), p.103-107</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-b1baebac5195cebb99eea041647a78469a208994144a085e24db43f3f48697d43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2001-11503.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1055/s-2001-11503$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,776,780,3004,3005,27903,27904,54537,54538</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=921249$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11301852$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koo, Tae Hyeon</creatorcontrib><creatorcontrib>Lee, Jeong-Hyung</creatorcontrib><creatorcontrib>Park, Yun Joo</creatorcontrib><creatorcontrib>Hong, Young-Soo</creatorcontrib><creatorcontrib>Kim, Hang Sub</creatorcontrib><creatorcontrib>Kim, Kyu-Won</creatorcontrib><creatorcontrib>Lee, Jung Joon</creatorcontrib><title>A Sesquiterpene Lactone, Costunolide, from Magnolia grandiflora Inhibits NF-κB by Targeting IκB Phosphorylation</title><title>Planta medica</title><addtitle>Planta Med</addtitle><description>Abstract A sesquiterpene lactone, costunolide (CTN), was identified from MAGNOLIA GRANDIFLORA together with parthenolide (PTN) by its strong inhibition of LPS-induced NF-κB activation. CTN, which showed more potent inhibition than PTN in the NF-κB activation, strongly suppressed nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. RT-PCR and Western blot analyses demonstrated that CTN suppressed the expression of iNOS mRNA and protein in a dose-dependent manner. CTN also significantly inhibited LPS-induced DNA-binding activity of NF-κB as well as the LPS-induced degradation of IκB-α and -β. Furthermore, CTN inhibited LPS-induced phosphorylation of IκB-α. These findings support that CTN inhibits NO production by down-regulating iNOS expression, at least, in part through the inhibition of IκBs' phosphorylation and degradation, which are essential for the activation of NF-κB.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Gene Expression Regulation - drug effects</subject><subject>General pharmacology</subject><subject>I-kappa B Proteins - metabolism</subject><subject>Lipopolysaccharides - metabolism</subject><subject>Macrophages - drug effects</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Molecular Structure</subject><subject>NF-kappa B - antagonists &amp; inhibitors</subject><subject>NF-kappa B - metabolism</subject><subject>Nitric Oxide - antagonists &amp; inhibitors</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>Nitric Oxide Synthase Type II</subject><subject>Original Paper</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Phosphorylation</subject><subject>Plant Leaves - chemistry</subject><subject>Plants, Medicinal - chemistry</subject><subject>Sesquiterpenes - isolation &amp; purification</subject><subject>Sesquiterpenes - pharmacology</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0M1uEzEQwHELgWgo3DgjS0hc6BZ_ZtfHNqIQKQUkytma3Z1NXO3aie095NX6EDxTN01ULpyssX6akf6EvOfskjOtv6RCMMYLzjWTL8iMK2kKJgR_SWaMSVEwo-QZeZPS_cSUYew1OeNcMl5pMSO7K_ob0250GeMWPdIVNDl4vKCLkPLoQ-_aaehiGOgtrA8z0HUE37quDxHo0m9c7XKiP26Kvw_XtN7TO4hrzM6v6fLw82sT0nYT4r6H7IJ_S1510Cd8d3rPyZ-br3eL78Xq57fl4mpVNFLzXNS8Bqyh0dzoBuvaGERgis9VCWWl5gYEq4xRXClglUah2lrJTnaqmpuyVfKcfDru3cawGzFlO7jUYN-DxzAmW5ZMS6PmE7w4wiaGlCJ2dhvdAHFvObOHxDbZQ2L7lHjiH057x3rA9h8-NZ3AxxOA1EDfTbEal56dEVwoM6nPR5U3Dge092GMfurx_6OPJYWSAQ</recordid><startdate>20010301</startdate><enddate>20010301</enddate><creator>Koo, Tae Hyeon</creator><creator>Lee, Jeong-Hyung</creator><creator>Park, Yun Joo</creator><creator>Hong, Young-Soo</creator><creator>Kim, Hang Sub</creator><creator>Kim, Kyu-Won</creator><creator>Lee, Jung Joon</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010301</creationdate><title>A Sesquiterpene Lactone, Costunolide, from Magnolia grandiflora Inhibits NF-κB by Targeting IκB Phosphorylation</title><author>Koo, Tae Hyeon ; Lee, Jeong-Hyung ; Park, Yun Joo ; Hong, Young-Soo ; Kim, Hang Sub ; Kim, Kyu-Won ; Lee, Jung Joon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-b1baebac5195cebb99eea041647a78469a208994144a085e24db43f3f48697d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Gene Expression Regulation - drug effects</topic><topic>General pharmacology</topic><topic>I-kappa B Proteins - metabolism</topic><topic>Lipopolysaccharides - metabolism</topic><topic>Macrophages - drug effects</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Molecular Structure</topic><topic>NF-kappa B - antagonists &amp; inhibitors</topic><topic>NF-kappa B - metabolism</topic><topic>Nitric Oxide - antagonists &amp; inhibitors</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>Nitric Oxide Synthase Type II</topic><topic>Original Paper</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Phosphorylation</topic><topic>Plant Leaves - chemistry</topic><topic>Plants, Medicinal - chemistry</topic><topic>Sesquiterpenes - isolation &amp; purification</topic><topic>Sesquiterpenes - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koo, Tae Hyeon</creatorcontrib><creatorcontrib>Lee, Jeong-Hyung</creatorcontrib><creatorcontrib>Park, Yun Joo</creatorcontrib><creatorcontrib>Hong, Young-Soo</creatorcontrib><creatorcontrib>Kim, Hang Sub</creatorcontrib><creatorcontrib>Kim, Kyu-Won</creatorcontrib><creatorcontrib>Lee, Jung Joon</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koo, Tae Hyeon</au><au>Lee, Jeong-Hyung</au><au>Park, Yun Joo</au><au>Hong, Young-Soo</au><au>Kim, Hang Sub</au><au>Kim, Kyu-Won</au><au>Lee, Jung Joon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Sesquiterpene Lactone, Costunolide, from Magnolia grandiflora Inhibits NF-κB by Targeting IκB Phosphorylation</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>2001-03-01</date><risdate>2001</risdate><volume>67</volume><issue>2</issue><spage>103</spage><epage>107</epage><pages>103-107</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract A sesquiterpene lactone, costunolide (CTN), was identified from MAGNOLIA GRANDIFLORA together with parthenolide (PTN) by its strong inhibition of LPS-induced NF-κB activation. CTN, which showed more potent inhibition than PTN in the NF-κB activation, strongly suppressed nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. RT-PCR and Western blot analyses demonstrated that CTN suppressed the expression of iNOS mRNA and protein in a dose-dependent manner. CTN also significantly inhibited LPS-induced DNA-binding activity of NF-κB as well as the LPS-induced degradation of IκB-α and -β. Furthermore, CTN inhibited LPS-induced phosphorylation of IκB-α. These findings support that CTN inhibits NO production by down-regulating iNOS expression, at least, in part through the inhibition of IκBs' phosphorylation and degradation, which are essential for the activation of NF-κB.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>11301852</pmid><doi>10.1055/s-2001-11503</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0032-0943
ispartof Planta medica, 2001-03, Vol.67 (2), p.103-107
issn 0032-0943
1439-0221
language eng
recordid cdi_proquest_miscellaneous_77053946
source MEDLINE; Thieme Connect Journals
subjects Animals
Biological and medical sciences
Cell Line - drug effects
Dose-Response Relationship, Drug
Gene Expression Regulation - drug effects
General pharmacology
I-kappa B Proteins - metabolism
Lipopolysaccharides - metabolism
Macrophages - drug effects
Medical sciences
Mice
Molecular Structure
NF-kappa B - antagonists & inhibitors
NF-kappa B - metabolism
Nitric Oxide - antagonists & inhibitors
Nitric Oxide - metabolism
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type II
Original Paper
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Phosphorylation
Plant Leaves - chemistry
Plants, Medicinal - chemistry
Sesquiterpenes - isolation & purification
Sesquiterpenes - pharmacology
title A Sesquiterpene Lactone, Costunolide, from Magnolia grandiflora Inhibits NF-κB by Targeting IκB Phosphorylation
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T00%3A40%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Sesquiterpene%20Lactone,%20Costunolide,%20from%20Magnolia%20grandiflora%20Inhibits%20NF-%CE%BAB%20by%20Targeting%20I%CE%BAB%20Phosphorylation&rft.jtitle=Planta%20medica&rft.au=Koo,%20Tae%20Hyeon&rft.date=2001-03-01&rft.volume=67&rft.issue=2&rft.spage=103&rft.epage=107&rft.pages=103-107&rft.issn=0032-0943&rft.eissn=1439-0221&rft.coden=PLMEAA&rft_id=info:doi/10.1055/s-2001-11503&rft_dat=%3Cproquest_cross%3E77053946%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77053946&rft_id=info:pmid/11301852&rfr_iscdi=true