Glucose uptake and phosphorylation in fat cells of fasted and hypophysectomized rats
Fat cells of hypophysectomized and fasted rats metabolize 10 times less glucose than adipocytes of normal rats in the presence of insulin. Glucose transport (3- O-methylglucose influx), transport plus phosphorylation (2-deoxyglucose uptake), hexokinase, pyruvate dehydrogenase and glucose-6-phosphate...
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| Veröffentlicht in: | Molecular and cellular endocrinology 1986-10, Vol.47 (3), p.217-224 |
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| creator | Bay, U. Froesch, E.R. |
| description | Fat cells of hypophysectomized and fasted rats metabolize 10 times less glucose than adipocytes of normal rats in the presence of insulin. Glucose transport (3-
O-methylglucose influx), transport plus phosphorylation (2-deoxyglucose uptake), hexokinase, pyruvate dehydrogenase and glucose-6-phosphate dehydrogenase activities were determined in an attempt to localize the metabolic defects.
Insulin stimulates 3-
O-methylglucose influx 5-fold in normal cells and 3-fold in cells of fasted rats. The basal influx in cells of fasted rats is increased and even more so in cells of hypophysectomized rats where the rate of basal influx is the same as that in cells of normal rats under maximal insulin stimulation. It cannot be further stimulated by insulin.
In contrast to 3-
O-methylglucose influx, basal uptake and phosphorylation of 2-deoxyglucose in cells of fasted and hypophysectomized rats is drastically decreased and stimulation by insulin is abolished.
Total hexokinase and pyruvate dehydrogenase activities are drastically reduced in the homogenate of fat cells of hypophysectomized and fasted rats. Phosphorylation by hexokinase appears to become one of the rate-limiting steps of glucose metabolism in cells of hypophysectomized rats. |
| doi_str_mv | 10.1016/0303-7207(86)90115-2 |
| format | Article |
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O-methylglucose influx), transport plus phosphorylation (2-deoxyglucose uptake), hexokinase, pyruvate dehydrogenase and glucose-6-phosphate dehydrogenase activities were determined in an attempt to localize the metabolic defects.
Insulin stimulates 3-
O-methylglucose influx 5-fold in normal cells and 3-fold in cells of fasted rats. The basal influx in cells of fasted rats is increased and even more so in cells of hypophysectomized rats where the rate of basal influx is the same as that in cells of normal rats under maximal insulin stimulation. It cannot be further stimulated by insulin.
In contrast to 3-
O-methylglucose influx, basal uptake and phosphorylation of 2-deoxyglucose in cells of fasted and hypophysectomized rats is drastically decreased and stimulation by insulin is abolished.
Total hexokinase and pyruvate dehydrogenase activities are drastically reduced in the homogenate of fat cells of hypophysectomized and fasted rats. Phosphorylation by hexokinase appears to become one of the rate-limiting steps of glucose metabolism in cells of hypophysectomized rats.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/0303-7207(86)90115-2</identifier><identifier>PMID: 3530835</identifier><identifier>CODEN: MCEND6</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>2-deoxyglucose ; 3- O-methylglucose ; adipocytes ; Adipose Tissue - metabolism ; Animals ; Biological and medical sciences ; Biological Transport ; Deoxyglucose - metabolism ; Fasting ; Fundamental and applied biological sciences. Psychology ; glucose ; Glucose - metabolism ; Glucosephosphate Dehydrogenase - metabolism ; hexokinase ; Hexokinase - metabolism ; Hypophysectomy ; insulin ; Insulin - pharmacology ; Methylglucosides - metabolism ; Phosphorylation ; pituitary ; Pyruvate Dehydrogenase Complex - metabolism ; Rats ; Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</subject><ispartof>Molecular and cellular endocrinology, 1986-10, Vol.47 (3), p.217-224</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-3f171bae5c68e5685d2ba911f38880f0784c5f06125e6e7128a788f573ed59463</citedby><cites>FETCH-LOGICAL-c417t-3f171bae5c68e5685d2ba911f38880f0784c5f06125e6e7128a788f573ed59463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0303720786901152$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8055818$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3530835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bay, U.</creatorcontrib><creatorcontrib>Froesch, E.R.</creatorcontrib><title>Glucose uptake and phosphorylation in fat cells of fasted and hypophysectomized rats</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>Fat cells of hypophysectomized and fasted rats metabolize 10 times less glucose than adipocytes of normal rats in the presence of insulin. Glucose transport (3-
O-methylglucose influx), transport plus phosphorylation (2-deoxyglucose uptake), hexokinase, pyruvate dehydrogenase and glucose-6-phosphate dehydrogenase activities were determined in an attempt to localize the metabolic defects.
Insulin stimulates 3-
O-methylglucose influx 5-fold in normal cells and 3-fold in cells of fasted rats. The basal influx in cells of fasted rats is increased and even more so in cells of hypophysectomized rats where the rate of basal influx is the same as that in cells of normal rats under maximal insulin stimulation. It cannot be further stimulated by insulin.
In contrast to 3-
O-methylglucose influx, basal uptake and phosphorylation of 2-deoxyglucose in cells of fasted and hypophysectomized rats is drastically decreased and stimulation by insulin is abolished.
Total hexokinase and pyruvate dehydrogenase activities are drastically reduced in the homogenate of fat cells of hypophysectomized and fasted rats. Phosphorylation by hexokinase appears to become one of the rate-limiting steps of glucose metabolism in cells of hypophysectomized rats.</description><subject>2-deoxyglucose</subject><subject>3- O-methylglucose</subject><subject>adipocytes</subject><subject>Adipose Tissue - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Deoxyglucose - metabolism</subject><subject>Fasting</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glucose</subject><subject>Glucose - metabolism</subject><subject>Glucosephosphate Dehydrogenase - metabolism</subject><subject>hexokinase</subject><subject>Hexokinase - metabolism</subject><subject>Hypophysectomy</subject><subject>insulin</subject><subject>Insulin - pharmacology</subject><subject>Methylglucosides - metabolism</subject><subject>Phosphorylation</subject><subject>pituitary</subject><subject>Pyruvate Dehydrogenase Complex - metabolism</subject><subject>Rats</subject><subject>Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFq3DAQQEVoSTdp_iAFH0pID041liXNXgIhtGkh0EtyFlp5xCr1Wq5kF7ZfX2122WMLGoQ0b4bhDWOXwG-Ag_rMBRe1bri-RvVpyQFk3ZywBaBuauRSv2GLI_KOneX8wjnXssFTdiqk4Cjkgj099LOLmap5nOxPquzQVeM65hJp29spxKEKQ-XtVDnq-1xFXx55ou4VXW_HOK63mdwUN-FP-U12yu_ZW2_7TBeH-5w9f_3ydP-tfvzx8P3-7rF2LeipFh40rCxJp5CkQtk1K7sE8AIRuecaWyc9V9BIUqShQasRvdSCOrlslThnV_u-Y4q_ZsqT2YS8G9MOFOdstOYSRDn_A6GVSuFSFLDdgy7FnBN5M6awsWlrgJuddbNTanZKDSrzat00pezDof-82lB3LDpoLvmPh7zNzvY-2cGFfMTKtiQCFux2j1GR9jtQMtkFGhx1IRXDpovh33P8BXcHnQk</recordid><startdate>19861001</startdate><enddate>19861001</enddate><creator>Bay, U.</creator><creator>Froesch, E.R.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19861001</creationdate><title>Glucose uptake and phosphorylation in fat cells of fasted and hypophysectomized rats</title><author>Bay, U. ; Froesch, E.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-3f171bae5c68e5685d2ba911f38880f0784c5f06125e6e7128a788f573ed59463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>2-deoxyglucose</topic><topic>3- O-methylglucose</topic><topic>adipocytes</topic><topic>Adipose Tissue - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Deoxyglucose - metabolism</topic><topic>Fasting</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glucose</topic><topic>Glucose - metabolism</topic><topic>Glucosephosphate Dehydrogenase - metabolism</topic><topic>hexokinase</topic><topic>Hexokinase - metabolism</topic><topic>Hypophysectomy</topic><topic>insulin</topic><topic>Insulin - pharmacology</topic><topic>Methylglucosides - metabolism</topic><topic>Phosphorylation</topic><topic>pituitary</topic><topic>Pyruvate Dehydrogenase Complex - metabolism</topic><topic>Rats</topic><topic>Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bay, U.</creatorcontrib><creatorcontrib>Froesch, E.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bay, U.</au><au>Froesch, E.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucose uptake and phosphorylation in fat cells of fasted and hypophysectomized rats</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>1986-10-01</date><risdate>1986</risdate><volume>47</volume><issue>3</issue><spage>217</spage><epage>224</epage><pages>217-224</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><coden>MCEND6</coden><abstract>Fat cells of hypophysectomized and fasted rats metabolize 10 times less glucose than adipocytes of normal rats in the presence of insulin. Glucose transport (3-
O-methylglucose influx), transport plus phosphorylation (2-deoxyglucose uptake), hexokinase, pyruvate dehydrogenase and glucose-6-phosphate dehydrogenase activities were determined in an attempt to localize the metabolic defects.
Insulin stimulates 3-
O-methylglucose influx 5-fold in normal cells and 3-fold in cells of fasted rats. The basal influx in cells of fasted rats is increased and even more so in cells of hypophysectomized rats where the rate of basal influx is the same as that in cells of normal rats under maximal insulin stimulation. It cannot be further stimulated by insulin.
In contrast to 3-
O-methylglucose influx, basal uptake and phosphorylation of 2-deoxyglucose in cells of fasted and hypophysectomized rats is drastically decreased and stimulation by insulin is abolished.
Total hexokinase and pyruvate dehydrogenase activities are drastically reduced in the homogenate of fat cells of hypophysectomized and fasted rats. Phosphorylation by hexokinase appears to become one of the rate-limiting steps of glucose metabolism in cells of hypophysectomized rats.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>3530835</pmid><doi>10.1016/0303-7207(86)90115-2</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular and cellular endocrinology, 1986-10, Vol.47 (3), p.217-224 |
| issn | 0303-7207 1872-8057 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 2-deoxyglucose 3- O-methylglucose adipocytes Adipose Tissue - metabolism Animals Biological and medical sciences Biological Transport Deoxyglucose - metabolism Fasting Fundamental and applied biological sciences. Psychology glucose Glucose - metabolism Glucosephosphate Dehydrogenase - metabolism hexokinase Hexokinase - metabolism Hypophysectomy insulin Insulin - pharmacology Methylglucosides - metabolism Phosphorylation pituitary Pyruvate Dehydrogenase Complex - metabolism Rats Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue |
| title | Glucose uptake and phosphorylation in fat cells of fasted and hypophysectomized rats |
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