Genetic Analysis of Amino Acid Accumulation in opaque-2 Maize Endosperm
The opaque-2 mutation in maize (Zea mays) is associated with an increased level of free amino acids (FAA) in the mature endosperm. In particular, there is a high concentration of lysine, the most limiting essential amino acid. To investigate the basis for the high-FAA phenotype of opaque-2 maize, we...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2001-04, Vol.125 (4), p.1766-1777 |
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| description | The opaque-2 mutation in maize (Zea mays) is associated with an increased level of free amino acids (FAA) in the mature endosperm. In particular, there is a high concentration of lysine, the most limiting essential amino acid. To investigate the basis for the high-FAA phenotype of opaque-2 maize, we characterized amino acid accumulation during endosperm development of several wild-type and opaque-2 inbreds. Oh545o2 was found to have an exceptionally high level of FAA, in particular those derived from aspartate (Asp) and intermediates of glycolysis. The FAA content in Oh545o2 is 12 times greater than its wild-type counterpart, and three and 10 times greater than in Oh51Ao2 and W64Ao2, respectively. We crossed Oh545o2 to Oh51Ao2 and analyzed the $\text{F}_{2\colon 3}$ progeny to identify genetic loci linked with the high FAA level in these mutants. Quantitative trait locus mapping identified four significant loci that account for about 46% of the phenotypic variance. One locus on the long arm of chromosome 2 is coincident with genes encoding a monofunctional Asp kinase 2 and a bifunctional Asp kinase-homo-Ser dehydrogenase-2, whereas another locus on the short arm of chromosome 3 is linked with a cytosolic triose phosphate isomerase 4. The results suggest an alternation of amino acid and carbon metabolism leads to overproduction and accumulation of FAA in opaque-2 mutants. |
| doi_str_mv | 10.1104/pp.125.4.1766 |
| format | Article |
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In particular, there is a high concentration of lysine, the most limiting essential amino acid. To investigate the basis for the high-FAA phenotype of opaque-2 maize, we characterized amino acid accumulation during endosperm development of several wild-type and opaque-2 inbreds. Oh545o2 was found to have an exceptionally high level of FAA, in particular those derived from aspartate (Asp) and intermediates of glycolysis. The FAA content in Oh545o2 is 12 times greater than its wild-type counterpart, and three and 10 times greater than in Oh51Ao2 and W64Ao2, respectively. We crossed Oh545o2 to Oh51Ao2 and analyzed the $\text{F}_{2\colon 3}$ progeny to identify genetic loci linked with the high FAA level in these mutants. Quantitative trait locus mapping identified four significant loci that account for about 46% of the phenotypic variance. One locus on the long arm of chromosome 2 is coincident with genes encoding a monofunctional Asp kinase 2 and a bifunctional Asp kinase-homo-Ser dehydrogenase-2, whereas another locus on the short arm of chromosome 3 is linked with a cytosolic triose phosphate isomerase 4. The results suggest an alternation of amino acid and carbon metabolism leads to overproduction and accumulation of FAA in opaque-2 mutants.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.125.4.1766</identifier><identifier>PMID: 11299357</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Accumulation ; Agronomy. Soil science and plant productions ; Amino acids ; Amino Acids - metabolism ; Biochemical Processes and Macromolecular Structures ; Biological and medical sciences ; Chromosome Mapping ; Chromosomes ; Corn ; Crosses, Genetic ; Economic plant physiology ; Endosperm ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Gene mapping ; Genetic loci ; Genetic mutation ; Hybridization, Genetic ; Inbreeding ; Metabolism ; Nitrogen metabolism ; Nitrogen metabolism and other ones (excepting carbon metabolism) ; Nutrition. Photosynthesis. Respiration. Metabolism ; Plant physiology and development ; Plants ; Quantitative trait loci ; Quantitative Trait, Heritable ; Seeds - metabolism ; Zea mays - genetics ; Zea mays - growth & development ; Zea mays - metabolism</subject><ispartof>Plant physiology (Bethesda), 2001-04, Vol.125 (4), p.1766-1777</ispartof><rights>Copyright 2001 American Society of Plant Physiologists</rights><rights>2001 INIST-CNRS</rights><rights>Copyright American Society of Plant Physiologists Apr 2001</rights><rights>Copyright © 2001, American Society of Plant Physiologists 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-8e3c837deb0a7d0e516d7eb840493b16a58de7b3c62251a7d085fe463b4aef2b3</citedby><cites>FETCH-LOGICAL-c455t-8e3c837deb0a7d0e516d7eb840493b16a58de7b3c62251a7d085fe463b4aef2b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4279808$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4279808$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=962870$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11299357$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xuelu</creatorcontrib><creatorcontrib>Larkins, Brian A.</creatorcontrib><title>Genetic Analysis of Amino Acid Accumulation in opaque-2 Maize Endosperm</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The opaque-2 mutation in maize (Zea mays) is associated with an increased level of free amino acids (FAA) in the mature endosperm. In particular, there is a high concentration of lysine, the most limiting essential amino acid. To investigate the basis for the high-FAA phenotype of opaque-2 maize, we characterized amino acid accumulation during endosperm development of several wild-type and opaque-2 inbreds. Oh545o2 was found to have an exceptionally high level of FAA, in particular those derived from aspartate (Asp) and intermediates of glycolysis. The FAA content in Oh545o2 is 12 times greater than its wild-type counterpart, and three and 10 times greater than in Oh51Ao2 and W64Ao2, respectively. We crossed Oh545o2 to Oh51Ao2 and analyzed the $\text{F}_{2\colon 3}$ progeny to identify genetic loci linked with the high FAA level in these mutants. Quantitative trait locus mapping identified four significant loci that account for about 46% of the phenotypic variance. One locus on the long arm of chromosome 2 is coincident with genes encoding a monofunctional Asp kinase 2 and a bifunctional Asp kinase-homo-Ser dehydrogenase-2, whereas another locus on the short arm of chromosome 3 is linked with a cytosolic triose phosphate isomerase 4. The results suggest an alternation of amino acid and carbon metabolism leads to overproduction and accumulation of FAA in opaque-2 mutants.</description><subject>Accumulation</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Amino acids</subject><subject>Amino Acids - metabolism</subject><subject>Biochemical Processes and Macromolecular Structures</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Corn</subject><subject>Crosses, Genetic</subject><subject>Economic plant physiology</subject><subject>Endosperm</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene mapping</subject><subject>Genetic loci</subject><subject>Genetic mutation</subject><subject>Hybridization, Genetic</subject><subject>Inbreeding</subject><subject>Metabolism</subject><subject>Nitrogen metabolism</subject><subject>Nitrogen metabolism and other ones (excepting carbon metabolism)</subject><subject>Nutrition. Photosynthesis. Respiration. Metabolism</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Quantitative trait loci</subject><subject>Quantitative Trait, Heritable</subject><subject>Seeds - metabolism</subject><subject>Zea mays - genetics</subject><subject>Zea mays - growth & development</subject><subject>Zea mays - metabolism</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpd0c9r2zAUB3AxNta023G3UkwLvTnTT0uGXkJp00LHLttZyPLzpmBLnmQX2r--yhKytRdJ8D5PfKWH0BeCl4Rg_nUcl4SKJV8SWVXv0IIIRksquHqPFhjnM1aqPkLHKW0wxoQR_hEdEULrmgm5QOs1eJicLVbe9E_JpSJ0xWpwPhQr69q82HmYezO54AvnizCaPzOUtPhm3DMUN74NaYQ4fEIfOtMn-LzfT9DP25sf13flw_f1_fXqobRciKlUwKxisoUGG9liEKRqJTSKY16zhlRGqBZkw2xFqSBbokQHvGINN9DRhp2gq92949wM0FrwUzS9HqMbTHzSwTj9uuLdb_0rPGqlFGO5_XLfHkN-R5r04JKFvjcewpy0lJgrJXCG52_gJswx_1HSlKi_6WhG5Q7ZGFKK0B1yEKy309HjqPN0NNfb6WR_9n_4f3o_jgwu9sAka_ouGm9dOri6okpus53u1CZNIR6qnMpaYcVeAJkjoK8</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>Wang, Xuelu</creator><creator>Larkins, Brian A.</creator><general>American Society of Plant Physiologists</general><general>American Society of Plant Biologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010401</creationdate><title>Genetic Analysis of Amino Acid Accumulation in opaque-2 Maize Endosperm</title><author>Wang, Xuelu ; Larkins, Brian A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-8e3c837deb0a7d0e516d7eb840493b16a58de7b3c62251a7d085fe463b4aef2b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Accumulation</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Amino acids</topic><topic>Amino Acids - metabolism</topic><topic>Biochemical Processes and Macromolecular Structures</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>Chromosomes</topic><topic>Corn</topic><topic>Crosses, Genetic</topic><topic>Economic plant physiology</topic><topic>Endosperm</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene mapping</topic><topic>Genetic loci</topic><topic>Genetic mutation</topic><topic>Hybridization, Genetic</topic><topic>Inbreeding</topic><topic>Metabolism</topic><topic>Nitrogen metabolism</topic><topic>Nitrogen metabolism and other ones (excepting carbon metabolism)</topic><topic>Nutrition. Photosynthesis. Respiration. Metabolism</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Quantitative trait loci</topic><topic>Quantitative Trait, Heritable</topic><topic>Seeds - metabolism</topic><topic>Zea mays - genetics</topic><topic>Zea mays - growth & development</topic><topic>Zea mays - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xuelu</creatorcontrib><creatorcontrib>Larkins, Brian A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xuelu</au><au>Larkins, Brian A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic Analysis of Amino Acid Accumulation in opaque-2 Maize Endosperm</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2001-04-01</date><risdate>2001</risdate><volume>125</volume><issue>4</issue><spage>1766</spage><epage>1777</epage><pages>1766-1777</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The opaque-2 mutation in maize (Zea mays) is associated with an increased level of free amino acids (FAA) in the mature endosperm. In particular, there is a high concentration of lysine, the most limiting essential amino acid. To investigate the basis for the high-FAA phenotype of opaque-2 maize, we characterized amino acid accumulation during endosperm development of several wild-type and opaque-2 inbreds. Oh545o2 was found to have an exceptionally high level of FAA, in particular those derived from aspartate (Asp) and intermediates of glycolysis. The FAA content in Oh545o2 is 12 times greater than its wild-type counterpart, and three and 10 times greater than in Oh51Ao2 and W64Ao2, respectively. We crossed Oh545o2 to Oh51Ao2 and analyzed the $\text{F}_{2\colon 3}$ progeny to identify genetic loci linked with the high FAA level in these mutants. Quantitative trait locus mapping identified four significant loci that account for about 46% of the phenotypic variance. One locus on the long arm of chromosome 2 is coincident with genes encoding a monofunctional Asp kinase 2 and a bifunctional Asp kinase-homo-Ser dehydrogenase-2, whereas another locus on the short arm of chromosome 3 is linked with a cytosolic triose phosphate isomerase 4. The results suggest an alternation of amino acid and carbon metabolism leads to overproduction and accumulation of FAA in opaque-2 mutants.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>11299357</pmid><doi>10.1104/pp.125.4.1766</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Accumulation Agronomy. Soil science and plant productions Amino acids Amino Acids - metabolism Biochemical Processes and Macromolecular Structures Biological and medical sciences Chromosome Mapping Chromosomes Corn Crosses, Genetic Economic plant physiology Endosperm Enzymes Fundamental and applied biological sciences. Psychology Gene mapping Genetic loci Genetic mutation Hybridization, Genetic Inbreeding Metabolism Nitrogen metabolism Nitrogen metabolism and other ones (excepting carbon metabolism) Nutrition. Photosynthesis. Respiration. Metabolism Plant physiology and development Plants Quantitative trait loci Quantitative Trait, Heritable Seeds - metabolism Zea mays - genetics Zea mays - growth & development Zea mays - metabolism |
| title | Genetic Analysis of Amino Acid Accumulation in opaque-2 Maize Endosperm |
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