31P-NMR studies of respiratory regulation in the intact myocardium
The mechanism by which mitochondrial respiration is coupled to ATP consumption in intact tissues is unclear. We determined the relationship between high-energy phosphate levels and oxygen consumption rate in rat hearts operating over a range of workloads and perfused with different substrates. With...
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| Veröffentlicht in: | FEBS letters 1986-10, Vol.206 (2), p.257-261 |
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| creator | From, Arthur H.L. Petein, Marc A. Michurski, Steven P. Zimmer, Stevan D. Uǧurbil, Kâmil |
| description | The mechanism by which mitochondrial respiration is coupled to ATP consumption in intact tissues is unclear. We determined the relationship between high-energy phosphate levels and oxygen consumption rate in rat hearts operating over a range of workloads and perfused with different substrates. With pyruvate + glucose perfusion, ADP levels were in general very low, and varied with MVO2 yielding an apparent Km of 25 ± 5 μM, suggesting regulation of oxidative phosphorylation through availability of ADP. In contrast, with glucose perfusion in the presence or absence of insulin, ADP levels, ADP/ATP ratio or the phosphate potential were relatively constant over the workload range examined and generally not correlated with alterations in MVO2; it is suggested that under these conditions, carbon substrate delivery to the mitochondria may control mitochondrial respiration. The common feature of both of the suggested regulatory mechanisms is substrate limitation which, however, is exercised at different metabolic points depending on the carbon substrate available to the myocardium. |
| doi_str_mv | 10.1016/0014-5793(86)80992-9 |
| format | Article |
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We determined the relationship between high-energy phosphate levels and oxygen consumption rate in rat hearts operating over a range of workloads and perfused with different substrates. With pyruvate + glucose perfusion, ADP levels were in general very low, and varied with MVO2 yielding an apparent Km of 25 ± 5 μM, suggesting regulation of oxidative phosphorylation through availability of ADP. In contrast, with glucose perfusion in the presence or absence of insulin, ADP levels, ADP/ATP ratio or the phosphate potential were relatively constant over the workload range examined and generally not correlated with alterations in MVO2; it is suggested that under these conditions, carbon substrate delivery to the mitochondria may control mitochondrial respiration. 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Psychology ; Glucose - metabolism ; Heart ; Insulin - pharmacology ; Kinetics ; Magnetic Resonance Spectroscopy ; Mitochondria - metabolism ; Myocardium - metabolism ; Oxygen Consumption - drug effects ; Phosphates - metabolism ; Pyruvates - metabolism ; Pyruvic Acid ; Rat myocardium ; Rats ; Respiratory regulation ; Substrate dependence ; Vertebrates: cardiovascular system</subject><ispartof>FEBS letters, 1986-10, Vol.206 (2), p.257-261</ispartof><rights>1986</rights><rights>FEBS Letters 206 (1986) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(86)80992-9$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8218433$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3530811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>From, Arthur H.L.</creatorcontrib><creatorcontrib>Petein, Marc A.</creatorcontrib><creatorcontrib>Michurski, Steven P.</creatorcontrib><creatorcontrib>Zimmer, Stevan D.</creatorcontrib><creatorcontrib>Uǧurbil, Kâmil</creatorcontrib><title>31P-NMR studies of respiratory regulation in the intact myocardium</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The mechanism by which mitochondrial respiration is coupled to ATP consumption in intact tissues is unclear. We determined the relationship between high-energy phosphate levels and oxygen consumption rate in rat hearts operating over a range of workloads and perfused with different substrates. With pyruvate + glucose perfusion, ADP levels were in general very low, and varied with MVO2 yielding an apparent Km of 25 ± 5 μM, suggesting regulation of oxidative phosphorylation through availability of ADP. In contrast, with glucose perfusion in the presence or absence of insulin, ADP levels, ADP/ATP ratio or the phosphate potential were relatively constant over the workload range examined and generally not correlated with alterations in MVO2; it is suggested that under these conditions, carbon substrate delivery to the mitochondria may control mitochondrial respiration. The common feature of both of the suggested regulatory mechanisms is substrate limitation which, however, is exercised at different metabolic points depending on the carbon substrate available to the myocardium.</description><subject>31P-NMR</subject><subject>Adenosine Diphosphate - metabolism</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>Heart</subject><subject>Insulin - pharmacology</subject><subject>Kinetics</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mitochondria - metabolism</subject><subject>Myocardium - metabolism</subject><subject>Oxygen Consumption - drug effects</subject><subject>Phosphates - metabolism</subject><subject>Pyruvates - metabolism</subject><subject>Pyruvic Acid</subject><subject>Rat myocardium</subject><subject>Rats</subject><subject>Respiratory regulation</subject><subject>Substrate dependence</subject><subject>Vertebrates: cardiovascular system</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEUhYMoWh__QGEWIroYzauZZCPY0qrgC9F1SJM7GplHTWaU_ntn2tKlq3vvOR8H7kHomOBLgom4wpjwdJgpdi7FhcRK0VRtoQGRGUsZF3IbDTbIHtqP8Qt3tyRqF-2yIcOSkAEaMfKSPj2-JrFpnYeY1HkSIM59ME0dFt3-0Ram8XWV-CppPqEbjbFNUi5qa4LzbXmIdnJTRDhazwP0Pp28je_Sh-fb-_HNQwqUC5WyGSjCcmmcBQqZG1JOpSMwy4wwRCx1rHLFHVGZlQCcWpbnFByjjLuMHaCzVe481N8txEaXPlooClNB3UadZZhLrkQHnqzBdlaC0_PgSxMWev1055-ufROtKfJgKuvjBpOUSM5Yh01X2K8vYLGxCdZ9_brvVvfdain0sn6t9HQyor3R61IsVdUFXa-CoGvnx0PQ0XqoLDgfwDba1f7fVPYH31mNcQ</recordid><startdate>19861006</startdate><enddate>19861006</enddate><creator>From, Arthur H.L.</creator><creator>Petein, Marc A.</creator><creator>Michurski, Steven P.</creator><creator>Zimmer, Stevan D.</creator><creator>Uǧurbil, Kâmil</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19861006</creationdate><title>31P-NMR studies of respiratory regulation in the intact myocardium</title><author>From, Arthur H.L. ; Petein, Marc A. ; Michurski, Steven P. ; Zimmer, Stevan D. ; Uǧurbil, Kâmil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e2469-3be913f8adce2e7d52428d1eb7a6a168adce09f94d197c8ee42c3ff2ed3234d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>31P-NMR</topic><topic>Adenosine Diphosphate - metabolism</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>Heart</topic><topic>Insulin - pharmacology</topic><topic>Kinetics</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Mitochondria - metabolism</topic><topic>Myocardium - metabolism</topic><topic>Oxygen Consumption - drug effects</topic><topic>Phosphates - metabolism</topic><topic>Pyruvates - metabolism</topic><topic>Pyruvic Acid</topic><topic>Rat myocardium</topic><topic>Rats</topic><topic>Respiratory regulation</topic><topic>Substrate dependence</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>From, Arthur H.L.</creatorcontrib><creatorcontrib>Petein, Marc A.</creatorcontrib><creatorcontrib>Michurski, Steven P.</creatorcontrib><creatorcontrib>Zimmer, Stevan D.</creatorcontrib><creatorcontrib>Uǧurbil, Kâmil</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>From, Arthur H.L.</au><au>Petein, Marc A.</au><au>Michurski, Steven P.</au><au>Zimmer, Stevan D.</au><au>Uǧurbil, Kâmil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>31P-NMR studies of respiratory regulation in the intact myocardium</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1986-10-06</date><risdate>1986</risdate><volume>206</volume><issue>2</issue><spage>257</spage><epage>261</epage><pages>257-261</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>The mechanism by which mitochondrial respiration is coupled to ATP consumption in intact tissues is unclear. We determined the relationship between high-energy phosphate levels and oxygen consumption rate in rat hearts operating over a range of workloads and perfused with different substrates. With pyruvate + glucose perfusion, ADP levels were in general very low, and varied with MVO2 yielding an apparent Km of 25 ± 5 μM, suggesting regulation of oxidative phosphorylation through availability of ADP. In contrast, with glucose perfusion in the presence or absence of insulin, ADP levels, ADP/ATP ratio or the phosphate potential were relatively constant over the workload range examined and generally not correlated with alterations in MVO2; it is suggested that under these conditions, carbon substrate delivery to the mitochondria may control mitochondrial respiration. The common feature of both of the suggested regulatory mechanisms is substrate limitation which, however, is exercised at different metabolic points depending on the carbon substrate available to the myocardium.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3530811</pmid><doi>10.1016/0014-5793(86)80992-9</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1986-10, Vol.206 (2), p.257-261 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via ScienceDirect (Elsevier); Alma/SFX Local Collection |
| subjects | 31P-NMR Adenosine Diphosphate - metabolism Adenosine Triphosphate - metabolism Animals Biological and medical sciences Fundamental and applied biological sciences. Psychology Glucose - metabolism Heart Insulin - pharmacology Kinetics Magnetic Resonance Spectroscopy Mitochondria - metabolism Myocardium - metabolism Oxygen Consumption - drug effects Phosphates - metabolism Pyruvates - metabolism Pyruvic Acid Rat myocardium Rats Respiratory regulation Substrate dependence Vertebrates: cardiovascular system |
| title | 31P-NMR studies of respiratory regulation in the intact myocardium |
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