Dendritic cells pulsed with unfractionated helminthic proteins to generate antiparasitic cytotoxic T lymphocyte
Dendritic cells (DC) are sentinels of immunity. We determined their role in the induction of immunity against alveolar echinococcosis, caused by the larval stage of the cestode Echinococcus multilocularis. Furthermore, we evaluated if unfractionated protein from E. multilocularis (Em‐Ag) can be used...
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| Veröffentlicht in: | Parasite immunology 2001-04, Vol.23 (4), p.195-201 |
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| creator | Jenne, Lars Arrighi, Jean‐Francois Sauter, Birthe Kern, Peter |
| description | Dendritic cells (DC) are sentinels of immunity. We determined their role in the induction of immunity against alveolar echinococcosis, caused by the larval stage of the cestode Echinococcus multilocularis. Furthermore, we evaluated if unfractionated protein from E. multilocularis (Em‐Ag) can be used as loading agent for DC (comparable to unfractionated tumour proteins) in order to generate antiparasitic cytotoxic T lymphocyte (CTL). Interestingly, immature DC did not mature in the presence of 1 µg/ml Em‐Ag as analysed by FACS and mixed leucocyte reactions. Yet, their capacity to take up dextran was markedly reduced. Further maturation of immature Em‐Ag pulsed DC could be induced by proinflammatory cytokines. These mature DC were slightly better inducers of T cell proliferation when compared with unpulsed mature DC. Importantly, by repetetive stimulation of autologous CD8+ lymphocytes with the Em‐Ag pulsed mature DC, we were able to generate specifically proliferating CTL lines. Thus, immunotherapy with ex vivo generated Em‐Ag pulsed DC might be of benefit for patients inheriting this incurable disease. |
| doi_str_mv | 10.1046/j.1365-3024.2001.00374.x |
| format | Article |
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Thus, immunotherapy with ex vivo generated Em‐Ag pulsed DC might be of benefit for patients inheriting this incurable disease.</description><identifier>ISSN: 0141-9838</identifier><identifier>EISSN: 1365-3024</identifier><identifier>DOI: 10.1046/j.1365-3024.2001.00374.x</identifier><identifier>PMID: 11298296</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Antigen Presentation - immunology ; Antigens, Helminth - immunology ; Cell Count ; Cells, Cultured ; dendritic cells ; Dendritic Cells - cytology ; Dendritic Cells - immunology ; echinococcosis ; Echinococcus - immunology ; Echinococcus multilocularis ; Flow Cytometry ; Helminth Proteins - immunology ; human alveolar echinococcosis ; Humans ; immunotherapy ; T-Lymphocytes, Cytotoxic - immunology</subject><ispartof>Parasite immunology, 2001-04, Vol.23 (4), p.195-201</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3954-7c9506e2d384de3547e506c6297a118b8eae470f600caddf657ed34a3452f433</citedby><cites>FETCH-LOGICAL-c3954-7c9506e2d384de3547e506c6297a118b8eae470f600caddf657ed34a3452f433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-3024.2001.00374.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-3024.2001.00374.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27923,27924,45573,45574,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11298296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jenne, Lars</creatorcontrib><creatorcontrib>Arrighi, Jean‐Francois</creatorcontrib><creatorcontrib>Sauter, Birthe</creatorcontrib><creatorcontrib>Kern, Peter</creatorcontrib><title>Dendritic cells pulsed with unfractionated helminthic proteins to generate antiparasitic cytotoxic T lymphocyte</title><title>Parasite immunology</title><addtitle>Parasite Immunol</addtitle><description>Dendritic cells (DC) are sentinels of immunity. We determined their role in the induction of immunity against alveolar echinococcosis, caused by the larval stage of the cestode Echinococcus multilocularis. Furthermore, we evaluated if unfractionated protein from E. multilocularis (Em‐Ag) can be used as loading agent for DC (comparable to unfractionated tumour proteins) in order to generate antiparasitic cytotoxic T lymphocyte (CTL). Interestingly, immature DC did not mature in the presence of 1 µg/ml Em‐Ag as analysed by FACS and mixed leucocyte reactions. Yet, their capacity to take up dextran was markedly reduced. Further maturation of immature Em‐Ag pulsed DC could be induced by proinflammatory cytokines. These mature DC were slightly better inducers of T cell proliferation when compared with unpulsed mature DC. Importantly, by repetetive stimulation of autologous CD8+ lymphocytes with the Em‐Ag pulsed mature DC, we were able to generate specifically proliferating CTL lines. Thus, immunotherapy with ex vivo generated Em‐Ag pulsed DC might be of benefit for patients inheriting this incurable disease.</description><subject>Animals</subject><subject>Antigen Presentation - immunology</subject><subject>Antigens, Helminth - immunology</subject><subject>Cell Count</subject><subject>Cells, Cultured</subject><subject>dendritic cells</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - immunology</subject><subject>echinococcosis</subject><subject>Echinococcus - immunology</subject><subject>Echinococcus multilocularis</subject><subject>Flow Cytometry</subject><subject>Helminth Proteins - immunology</subject><subject>human alveolar echinococcosis</subject><subject>Humans</subject><subject>immunotherapy</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><issn>0141-9838</issn><issn>1365-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v3CAQhlGVqtmk_QsVp97s8GXAUi5V0nxIidLD3hGxx11WtnEAK7v_vri7ao7JiWF45p1hXoQwJSUlQl5sS8plVXDCRMkIoSUhXIly9wmt_j-coBWhgha15voUncW4zSBnkn9Bp5SyWrNarpC_hrENLrkGN9D3EU9zH6HFry5t8Dx2wTbJ-dGmnNtAP7gxbTI7BZ_AjREnj__ACCED2I7JTTbYeJDbJ5_8Lkdr3O-HaeNzBr6iz53NHb4dz3O0vvm1vrorHp5u769-PhQNrytRqKauiATWci1a4JVQkO-NZLWylOpnDRaEIp0kpLFt28lKQcuF5aJineD8HP04yOZBX2aIyQwuLh-0I_g5GqWI0EKwd0GqmVCVlhnUB7AJPsYAnZmCG2zYG0rMYorZmmX3Ztm9WUwx_0wxu1z6_dhjfh6gfSs8upCBywPw6nrYf1jY_L5_zAH_C-SNnOg</recordid><startdate>200104</startdate><enddate>200104</enddate><creator>Jenne, Lars</creator><creator>Arrighi, Jean‐Francois</creator><creator>Sauter, Birthe</creator><creator>Kern, Peter</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200104</creationdate><title>Dendritic cells pulsed with unfractionated helminthic proteins to generate antiparasitic cytotoxic T lymphocyte</title><author>Jenne, Lars ; Arrighi, Jean‐Francois ; Sauter, Birthe ; Kern, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3954-7c9506e2d384de3547e506c6297a118b8eae470f600caddf657ed34a3452f433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Antigen Presentation - immunology</topic><topic>Antigens, Helminth - immunology</topic><topic>Cell Count</topic><topic>Cells, Cultured</topic><topic>dendritic cells</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - immunology</topic><topic>echinococcosis</topic><topic>Echinococcus - immunology</topic><topic>Echinococcus multilocularis</topic><topic>Flow Cytometry</topic><topic>Helminth Proteins - immunology</topic><topic>human alveolar echinococcosis</topic><topic>Humans</topic><topic>immunotherapy</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jenne, Lars</creatorcontrib><creatorcontrib>Arrighi, Jean‐Francois</creatorcontrib><creatorcontrib>Sauter, Birthe</creatorcontrib><creatorcontrib>Kern, Peter</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasite immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jenne, Lars</au><au>Arrighi, Jean‐Francois</au><au>Sauter, Birthe</au><au>Kern, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dendritic cells pulsed with unfractionated helminthic proteins to generate antiparasitic cytotoxic T lymphocyte</atitle><jtitle>Parasite immunology</jtitle><addtitle>Parasite Immunol</addtitle><date>2001-04</date><risdate>2001</risdate><volume>23</volume><issue>4</issue><spage>195</spage><epage>201</epage><pages>195-201</pages><issn>0141-9838</issn><eissn>1365-3024</eissn><abstract>Dendritic cells (DC) are sentinels of immunity. We determined their role in the induction of immunity against alveolar echinococcosis, caused by the larval stage of the cestode Echinococcus multilocularis. Furthermore, we evaluated if unfractionated protein from E. multilocularis (Em‐Ag) can be used as loading agent for DC (comparable to unfractionated tumour proteins) in order to generate antiparasitic cytotoxic T lymphocyte (CTL). Interestingly, immature DC did not mature in the presence of 1 µg/ml Em‐Ag as analysed by FACS and mixed leucocyte reactions. Yet, their capacity to take up dextran was markedly reduced. Further maturation of immature Em‐Ag pulsed DC could be induced by proinflammatory cytokines. These mature DC were slightly better inducers of T cell proliferation when compared with unpulsed mature DC. Importantly, by repetetive stimulation of autologous CD8+ lymphocytes with the Em‐Ag pulsed mature DC, we were able to generate specifically proliferating CTL lines. Thus, immunotherapy with ex vivo generated Em‐Ag pulsed DC might be of benefit for patients inheriting this incurable disease.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>11298296</pmid><doi>10.1046/j.1365-3024.2001.00374.x</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Wiley Free Content; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Animals Antigen Presentation - immunology Antigens, Helminth - immunology Cell Count Cells, Cultured dendritic cells Dendritic Cells - cytology Dendritic Cells - immunology echinococcosis Echinococcus - immunology Echinococcus multilocularis Flow Cytometry Helminth Proteins - immunology human alveolar echinococcosis Humans immunotherapy T-Lymphocytes, Cytotoxic - immunology |
| title | Dendritic cells pulsed with unfractionated helminthic proteins to generate antiparasitic cytotoxic T lymphocyte |
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