A strategy for optimizing quality and quantity of DNA extracted from soil

The efficiency of a bead beating method was studied in detail with regard to a variety of factors including beating time and speed, volume and temperature of the buffer, as well as amount and type of beads employed. The results presented here reveal that all of these parameters have a significant ef...

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Veröffentlicht in:	Journal of microbiological methods 2001-05, Vol.45 (1), p.7-20
Hauptverfasser:	Bürgmann, Helmut, Pesaro, Manuel, Widmer, Franco, Zeyer, Josef
Format:	Artikel
Sprache:	eng
Schlagworte:	Bead beating Biological and medical sciences Biotechnology Buffers DNA DNA - analysis DNA - isolation & purification DNA, Bacterial - analysis DNA, Bacterial - isolation & purification Extraction Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Miscellaneous Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Soil Soil - analysis Soil Microbiology Synthetic digonucleotides and genes. Sequencing Temperature
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