Na+-alanine uptake activates a Cl- conductance in frog renal proximal tubule cells via nonconventional PKC

Hyposmotically induced swelling of frog renal proximal tubule cells activates a DIDS-sensitive, outwardly rectifying Cl- conductance via a conventional protein kinase C (PKC). This study examines whether Na+-alanine cotransport similarly activates a DIDS-sensitive Cl- conductance in frog renal proxi...

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Veröffentlicht in:American journal of physiology. Renal physiology 2001-05, Vol.280 (5), p.F758-F767
Hauptverfasser: Millar, I D, Robson, L
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container_title American journal of physiology. Renal physiology
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creator Millar, I D
Robson, L
description Hyposmotically induced swelling of frog renal proximal tubule cells activates a DIDS-sensitive, outwardly rectifying Cl- conductance via a conventional protein kinase C (PKC). This study examines whether Na+-alanine cotransport similarly activates a DIDS-sensitive Cl- conductance in frog renal proximal tubule cells. On stimulation of Na+-alanine cotransport, the DIDS-sensitive current (I(DIDS-Ala)) increased markedly over time. I(DIDS-Ala) exhibited outward rectification, a Na+/Cl- selectivity ratio of 0.19 +/- 0.03, and an anion selectivity sequence Br- = Cl- > I- > gluconate-. Activation of I(DIDS-Ala) was dependent on ATP hydrolysis and PKC-mediated phosphorylation and was inhibited by hyperosmotic conditions. Activation could be not ascribed to a conventional PKC isoform, as I(DIDS-Ala) was not affected by removing Ca2+ or by phorbol ester treatment, suggesting a role for a nonconventional PKC isoform, either novel or atypical. We conclude that Na+-alanine cotransport activates a DIDS-sensitive Cl- conductance via a nonconventional PKC isoform. This contrasts with the hyposmotically activated Cl- conductance, which requires conventional PKC activation.
doi_str_mv 10.1152/ajprenal.2001.280.5.f758
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Activation could be not ascribed to a conventional PKC isoform, as I(DIDS-Ala) was not affected by removing Ca2+ or by phorbol ester treatment, suggesting a role for a nonconventional PKC isoform, either novel or atypical. We conclude that Na+-alanine cotransport activates a DIDS-sensitive Cl- conductance via a nonconventional PKC isoform. 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Renal physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Millar, I D</au><au>Robson, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Na+-alanine uptake activates a Cl- conductance in frog renal proximal tubule cells via nonconventional PKC</atitle><jtitle>American journal of physiology. Renal physiology</jtitle><addtitle>Am J Physiol Renal Physiol</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>280</volume><issue>5</issue><spage>F758</spage><epage>F767</epage><pages>F758-F767</pages><issn>1931-857X</issn><eissn>1522-1466</eissn><abstract>Hyposmotically induced swelling of frog renal proximal tubule cells activates a DIDS-sensitive, outwardly rectifying Cl- conductance via a conventional protein kinase C (PKC). This study examines whether Na+-alanine cotransport similarly activates a DIDS-sensitive Cl- conductance in frog renal proximal tubule cells. On stimulation of Na+-alanine cotransport, the DIDS-sensitive current (I(DIDS-Ala)) increased markedly over time. I(DIDS-Ala) exhibited outward rectification, a Na+/Cl- selectivity ratio of 0.19 +/- 0.03, and an anion selectivity sequence Br- = Cl- &gt; I- &gt; gluconate-. Activation of I(DIDS-Ala) was dependent on ATP hydrolysis and PKC-mediated phosphorylation and was inhibited by hyperosmotic conditions. Activation could be not ascribed to a conventional PKC isoform, as I(DIDS-Ala) was not affected by removing Ca2+ or by phorbol ester treatment, suggesting a role for a nonconventional PKC isoform, either novel or atypical. We conclude that Na+-alanine cotransport activates a DIDS-sensitive Cl- conductance via a nonconventional PKC isoform. This contrasts with the hyposmotically activated Cl- conductance, which requires conventional PKC activation.</abstract><cop>United States</cop><pmid>11292617</pmid><doi>10.1152/ajprenal.2001.280.5.f758</doi></addata></record>
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source MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals
subjects 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology
Adenosine Triphosphate - physiology
Alanine - metabolism
Alanine - pharmacology
Algorithms
Animals
Chloride Channels - drug effects
Chloride Channels - metabolism
Humans
In Vitro Techniques
Kidney Tubules, Proximal - cytology
Kidney Tubules, Proximal - enzymology
Kidney Tubules, Proximal - metabolism
Muscle Contraction - drug effects
Osmolar Concentration
Patch-Clamp Techniques
Protein Kinase C - metabolism
Rana temporaria
Sodium - metabolism
Stereoisomerism
title Na+-alanine uptake activates a Cl- conductance in frog renal proximal tubule cells via nonconventional PKC
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