Mouse MCT3 gene is expressed preferentially in retinal pigment and choroid plexus epithelia
1 Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia 19107; and 2 Department of Basic Sciences, Pennsylvania College of Optometry, Elkins Park, Pennsylvania 19027 Monocarboxylate transporters (MCTs) are a family of highly homologous membrane proteins that...
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| Veröffentlicht in: | American Journal of Physiology: Cell Physiology 2001-05, Vol.280 (5), p.C1319-C1326 |
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| container_title | American Journal of Physiology: Cell Physiology |
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| creator | Philp, Nancy J Yoon, Heeyong Lombardi, Lorraine |
| description | 1 Department of Pathology, Anatomy, and Cell Biology, Thomas
Jefferson University, Philadelphia 19107; and 2 Department
of Basic Sciences, Pennsylvania College of Optometry, Elkins Park,
Pennsylvania 19027
Monocarboxylate transporters (MCTs)
are a family of highly homologous membrane proteins that mediate the
1:1 transport of a proton and a lactate ion. In chicken, MCT3 is
preferentially expressed in the retinal pigment epithelium (RPE). We
have isolated the mouse MCT3 cDNA and gene and characterized the
pattern of tissue expression. MCT3 is a single copy gene with a 1.8-kb
transcript that encodes a protein with a predicted molecular
mass of 51.5 kDa. Based on Northern hybridization analysis,
MCT3 transcript was expressed in only two tissues: RPE and choroid
plexus epithelium (CPE). The choroid plexus forms a barrier between the
cerebrospinal fluid and fenestrated capillaries, similar to the
organization of the RPE and choroidal vessels. Immunohistochemical
staining demonstrated that MCT3 was restricted to the basolateral
membranes of both epithelia but was more abundant in RPE than CPE.
Differences in the level of protein expression were confirmed by
Western blot analysis. The cloning of MCT3 identifies a specific
transporter that could regulate lactate levels in fluid-bathing
neuronal tissues.
retina; lactate; transporter; blood-tissues barrier; monocarboxylate transporter |
| doi_str_mv | 10.1152/ajpcell.2001.280.5.c1319 |
| format | Article |
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Jefferson University, Philadelphia 19107; and 2 Department
of Basic Sciences, Pennsylvania College of Optometry, Elkins Park,
Pennsylvania 19027
Monocarboxylate transporters (MCTs)
are a family of highly homologous membrane proteins that mediate the
1:1 transport of a proton and a lactate ion. In chicken, MCT3 is
preferentially expressed in the retinal pigment epithelium (RPE). We
have isolated the mouse MCT3 cDNA and gene and characterized the
pattern of tissue expression. MCT3 is a single copy gene with a 1.8-kb
transcript that encodes a protein with a predicted molecular
mass of 51.5 kDa. Based on Northern hybridization analysis,
MCT3 transcript was expressed in only two tissues: RPE and choroid
plexus epithelium (CPE). The choroid plexus forms a barrier between the
cerebrospinal fluid and fenestrated capillaries, similar to the
organization of the RPE and choroidal vessels. Immunohistochemical
staining demonstrated that MCT3 was restricted to the basolateral
membranes of both epithelia but was more abundant in RPE than CPE.
Differences in the level of protein expression were confirmed by
Western blot analysis. The cloning of MCT3 identifies a specific
transporter that could regulate lactate levels in fluid-bathing
neuronal tissues.
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Jefferson University, Philadelphia 19107; and 2 Department
of Basic Sciences, Pennsylvania College of Optometry, Elkins Park,
Pennsylvania 19027
Monocarboxylate transporters (MCTs)
are a family of highly homologous membrane proteins that mediate the
1:1 transport of a proton and a lactate ion. In chicken, MCT3 is
preferentially expressed in the retinal pigment epithelium (RPE). We
have isolated the mouse MCT3 cDNA and gene and characterized the
pattern of tissue expression. MCT3 is a single copy gene with a 1.8-kb
transcript that encodes a protein with a predicted molecular
mass of 51.5 kDa. Based on Northern hybridization analysis,
MCT3 transcript was expressed in only two tissues: RPE and choroid
plexus epithelium (CPE). The choroid plexus forms a barrier between the
cerebrospinal fluid and fenestrated capillaries, similar to the
organization of the RPE and choroidal vessels. Immunohistochemical
staining demonstrated that MCT3 was restricted to the basolateral
membranes of both epithelia but was more abundant in RPE than CPE.
Differences in the level of protein expression were confirmed by
Western blot analysis. The cloning of MCT3 identifies a specific
transporter that could regulate lactate levels in fluid-bathing
neuronal tissues.
retina; lactate; transporter; blood-tissues barrier; monocarboxylate transporter</description><subject>5' Untranslated Regions - genetics</subject><subject>Alternative Splicing</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Carrier Proteins - analysis</subject><subject>Carrier Proteins - chemistry</subject><subject>Carrier Proteins - genetics</subject><subject>Cell Membrane - metabolism</subject><subject>Chickens</subject><subject>Choroid Plexus - cytology</subject><subject>Choroid Plexus - physiology</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - physiology</subject><subject>Exons</subject><subject>Humans</subject><subject>Introns</subject><subject>Membrane Transport Proteins</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Monocarboxylic Acid Transporters</subject><subject>Organ Specificity</subject><subject>Pigment Epithelium of Eye - cytology</subject><subject>Pigment Epithelium of Eye - physiology</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Transcription, Genetic</subject><issn>0363-6143</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kL1OwzAURi0EoqXwCsgTW4J_kjhhQxUFJBBLmRgsO7lpjNwk2Ilo3x5XLQIGpitdf-da30EIUxJTmrJr9d6XYG3MCKExy0mcxiXltDhC0_DMIppm_BhNCc94lNGET9CZ9--EkIRlxSmaUMpywZN0it6eu9EDfp4vOV5BC9h4DJvegfdQ4TBrcNAORlm7xabFDgbTKot7s1qHPVZthcumc50JaQubMeC9GRqwRp2jk1pZDxeHOUOvi7vl_CF6erl_nN8-RWWSiSHKa15WiqeqSABoWZA6L4pEF5zVGSlZphWISoeqouKFzmmqdM6J0qCJEJpXfIau9nd7132M4Ae5Nn7nR7UQ2kkhCE8EYyGY74Ol67wP3WTvzFq5raRE7sTKg1i5EyuDWJnK-U5sQC8Pf4x6DdUPeDAZAvE-0JhV82kcyL7ZetPZbrX9Ofvn4s3_wGK0dgmb4Zv8Bcq-qvkXe9adXQ</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Philp, Nancy J</creator><creator>Yoon, Heeyong</creator><creator>Lombardi, Lorraine</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Mouse MCT3 gene is expressed preferentially in retinal pigment and choroid plexus epithelia</title><author>Philp, Nancy J ; Yoon, Heeyong ; Lombardi, Lorraine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-8f3cda35a94ee1c90f8994b932f60c26bae7db2007d39b815ab830abeb077b3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>5' Untranslated Regions - genetics</topic><topic>Alternative Splicing</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Carrier Proteins - analysis</topic><topic>Carrier Proteins - chemistry</topic><topic>Carrier Proteins - genetics</topic><topic>Cell Membrane - metabolism</topic><topic>Chickens</topic><topic>Choroid Plexus - cytology</topic><topic>Choroid Plexus - physiology</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - physiology</topic><topic>Exons</topic><topic>Humans</topic><topic>Introns</topic><topic>Membrane Transport Proteins</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Monocarboxylic Acid Transporters</topic><topic>Organ Specificity</topic><topic>Pigment Epithelium of Eye - cytology</topic><topic>Pigment Epithelium of Eye - physiology</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Philp, Nancy J</creatorcontrib><creatorcontrib>Yoon, Heeyong</creatorcontrib><creatorcontrib>Lombardi, Lorraine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Philp, Nancy J</au><au>Yoon, Heeyong</au><au>Lombardi, Lorraine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mouse MCT3 gene is expressed preferentially in retinal pigment and choroid plexus epithelia</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><addtitle>Am J Physiol Cell Physiol</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>280</volume><issue>5</issue><spage>C1319</spage><epage>C1326</epage><pages>C1319-C1326</pages><issn>0363-6143</issn><eissn>1522-1563</eissn><abstract>1 Department of Pathology, Anatomy, and Cell Biology, Thomas
Jefferson University, Philadelphia 19107; and 2 Department
of Basic Sciences, Pennsylvania College of Optometry, Elkins Park,
Pennsylvania 19027
Monocarboxylate transporters (MCTs)
are a family of highly homologous membrane proteins that mediate the
1:1 transport of a proton and a lactate ion. In chicken, MCT3 is
preferentially expressed in the retinal pigment epithelium (RPE). We
have isolated the mouse MCT3 cDNA and gene and characterized the
pattern of tissue expression. MCT3 is a single copy gene with a 1.8-kb
transcript that encodes a protein with a predicted molecular
mass of 51.5 kDa. Based on Northern hybridization analysis,
MCT3 transcript was expressed in only two tissues: RPE and choroid
plexus epithelium (CPE). The choroid plexus forms a barrier between the
cerebrospinal fluid and fenestrated capillaries, similar to the
organization of the RPE and choroidal vessels. Immunohistochemical
staining demonstrated that MCT3 was restricted to the basolateral
membranes of both epithelia but was more abundant in RPE than CPE.
Differences in the level of protein expression were confirmed by
Western blot analysis. The cloning of MCT3 identifies a specific
transporter that could regulate lactate levels in fluid-bathing
neuronal tissues.
retina; lactate; transporter; blood-tissues barrier; monocarboxylate transporter</abstract><cop>United States</cop><pmid>11287345</pmid><doi>10.1152/ajpcell.2001.280.5.c1319</doi></addata></record> |
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| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 5' Untranslated Regions - genetics Alternative Splicing Amino Acid Sequence Animals Base Sequence Carrier Proteins - analysis Carrier Proteins - chemistry Carrier Proteins - genetics Cell Membrane - metabolism Chickens Choroid Plexus - cytology Choroid Plexus - physiology Cloning, Molecular DNA, Complementary Epithelial Cells - cytology Epithelial Cells - physiology Exons Humans Introns Membrane Transport Proteins Mice Molecular Sequence Data Molecular Weight Monocarboxylic Acid Transporters Organ Specificity Pigment Epithelium of Eye - cytology Pigment Epithelium of Eye - physiology RNA, Messenger - genetics Sequence Alignment Sequence Homology, Amino Acid Transcription, Genetic |
| title | Mouse MCT3 gene is expressed preferentially in retinal pigment and choroid plexus epithelia |
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