Expression of single-chain Fv-Fc fusions in Pichia pastoris

Phage display technology makes possible the direct isolation of monovalent single-chain Fv antibody fragments. For many applications, however, it is useful to restore Fc mediated antibody functions such as avidity, effector functions and a prolonged serum half-life. We have constructed vectors for t...

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Veröffentlicht in:	Journal of immunological methods 2001-05, Vol.251 (1), p.123-135
Hauptverfasser:	Powers, David B., Amersdorfer, Peter, Poul, Marie-Alix, Nielsen, Ulrik B., Shalaby, M.Refaat, Adams, Gregory P., Weiner, Louis M., Marks, James D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibody engineering Antibody-Dependent Cell Cytotoxicity Base Sequence Biological and medical sciences Biotechnology Botulism DNA Primers - genetics Fundamental and applied biological sciences. Psychology Fundamental immunology Genetic Vectors Health. Pharmaceutical industry HER2/neu Humans Immunoglobulin Fc Fragments - biosynthesis Immunoglobulin Fc Fragments - genetics Immunoglobulin Fc Fragments - isolation & purification Immunoglobulin Fragments - biosynthesis Immunoglobulin Fragments - genetics Immunoglobulin Fragments - isolation & purification Immunoglobulin G - biosynthesis Immunoglobulin G - genetics Immunoglobulin G - isolation & purification immunoglobulin G1 Industrial applications and implications. Economical aspects Male Mice Mice, SCID Microbiology Molecular immunology Molecular Sequence Data Monoclonal antibodies Peptide Library Phage display Pichia - genetics Pichia pastoris Plasmids - genetics Production of active biomolecules Protein Engineering Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification scFv Techniques
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