Regulation of cell proliferation, inflammatory cytokine production and calcium mobilization in primary human T lymphocytes by emodin from Polygonum hypoleucum Ohwi
This study was designed to elucidate action mechanisms of four anthraquinones identified from Polygonum hypoleucum Ohwi (P. hypoleucum Ohwi) on primary human T lymphocytes. The cells were isolated from peripheral blood. T cells were treated with 5 to 60 microM of four anthraquinones with or without...
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| Veröffentlicht in: | Inflammation research 2001-02, Vol.50 (2), p.73-82 |
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| container_title | Inflammation research |
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| creator | Kuo, Y C Meng, H C Tsai, W J |
| description | This study was designed to elucidate action mechanisms of four anthraquinones identified from Polygonum hypoleucum Ohwi (P. hypoleucum Ohwi) on primary human T lymphocytes.
The cells were isolated from peripheral blood.
T cells were treated with 5 to 60 microM of four anthraquinones with or without phytohemagglutinin (PHA; 5 microg/ml) for 3 days. Effects of 4 anthraquinones on T lymphocyte proliferation, production and gene expression of inflammatory cytokines and intracellular free Ca2+ concentration ([Ca2+]i) were determined. Data were assessed with Student's t-test.
On a percentage basis, emodin had the highest suppressing activity on T lymphocyte proliferation with an IC50 of 11.2 +/- 0.6 microM. Emodin decreased cytokine production, IL-2 mRNA expression, and [Ca2+]i in activated T cells.
We hypothesize that the inhibitory mechanisms of emodin on activated T cells proliferation are related to the impairment of cytokine production, IL-2 mRNA level and [Ca2+]i in the cells. |
| doi_str_mv | 10.1007/s000110050727 |
| format | Article |
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The cells were isolated from peripheral blood.
T cells were treated with 5 to 60 microM of four anthraquinones with or without phytohemagglutinin (PHA; 5 microg/ml) for 3 days. Effects of 4 anthraquinones on T lymphocyte proliferation, production and gene expression of inflammatory cytokines and intracellular free Ca2+ concentration ([Ca2+]i) were determined. Data were assessed with Student's t-test.
On a percentage basis, emodin had the highest suppressing activity on T lymphocyte proliferation with an IC50 of 11.2 +/- 0.6 microM. Emodin decreased cytokine production, IL-2 mRNA expression, and [Ca2+]i in activated T cells.
We hypothesize that the inhibitory mechanisms of emodin on activated T cells proliferation are related to the impairment of cytokine production, IL-2 mRNA level and [Ca2+]i in the cells.</description><identifier>ISSN: 1023-3830</identifier><identifier>EISSN: 1420-908X</identifier><identifier>DOI: 10.1007/s000110050727</identifier><identifier>PMID: 11289657</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Adult ; anthraquinones ; Calcium - metabolism ; Cell Division - drug effects ; Cytokines - biosynthesis ; emodin ; Emodin - administration & dosage ; Emodin - pharmacology ; Gene Expression - drug effects ; Humans ; Interleukin-2 - genetics ; Lymphocyte Activation - drug effects ; Male ; Phytohemagglutinins - pharmacology ; Plant Lectins ; Polygonaceae - chemistry ; Polygonum ; Polygonum hypoleucum ; RNA, Messenger - analysis ; T-Lymphocytes - cytology ; T-Lymphocytes - drug effects ; T-Lymphocytes - metabolism</subject><ispartof>Inflammation research, 2001-02, Vol.50 (2), p.73-82</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-77a74ff2a1d765517c9b174e06e316590e01461e7e0c279b3d13804a559afc8d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11289657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuo, Y C</creatorcontrib><creatorcontrib>Meng, H C</creatorcontrib><creatorcontrib>Tsai, W J</creatorcontrib><title>Regulation of cell proliferation, inflammatory cytokine production and calcium mobilization in primary human T lymphocytes by emodin from Polygonum hypoleucum Ohwi</title><title>Inflammation research</title><addtitle>Inflamm Res</addtitle><description>This study was designed to elucidate action mechanisms of four anthraquinones identified from Polygonum hypoleucum Ohwi (P. hypoleucum Ohwi) on primary human T lymphocytes.
The cells were isolated from peripheral blood.
T cells were treated with 5 to 60 microM of four anthraquinones with or without phytohemagglutinin (PHA; 5 microg/ml) for 3 days. Effects of 4 anthraquinones on T lymphocyte proliferation, production and gene expression of inflammatory cytokines and intracellular free Ca2+ concentration ([Ca2+]i) were determined. Data were assessed with Student's t-test.
On a percentage basis, emodin had the highest suppressing activity on T lymphocyte proliferation with an IC50 of 11.2 +/- 0.6 microM. Emodin decreased cytokine production, IL-2 mRNA expression, and [Ca2+]i in activated T cells.
We hypothesize that the inhibitory mechanisms of emodin on activated T cells proliferation are related to the impairment of cytokine production, IL-2 mRNA level and [Ca2+]i in the cells.</description><subject>Adult</subject><subject>anthraquinones</subject><subject>Calcium - metabolism</subject><subject>Cell Division - drug effects</subject><subject>Cytokines - biosynthesis</subject><subject>emodin</subject><subject>Emodin - administration & dosage</subject><subject>Emodin - pharmacology</subject><subject>Gene Expression - drug effects</subject><subject>Humans</subject><subject>Interleukin-2 - genetics</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Male</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>Plant Lectins</subject><subject>Polygonaceae - chemistry</subject><subject>Polygonum</subject><subject>Polygonum hypoleucum</subject><subject>RNA, Messenger - analysis</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - metabolism</subject><issn>1023-3830</issn><issn>1420-908X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1q3DAURkVpyF-z7DZoVbqo2yvJ9pWXJaRNIZAQUujOyLKUUSJZE8umOK_TF40mM1C6SVf6EOcepPsR8p7BZwaAXxIAsJwqQI5vyCErORQNyF9vcwYuCiEFHJCjlO4zKbnk--SAMS6busJD8ufG3M1eTS4ONFqqjfd0PUbvrBlfbj9RN1ivQlBTHBeqlyk-uMFsoH7WL3Nq6KlWXrs50BA7593TVuiGjLmg8txqDmqgt9QvYb2K2WIS7RZqQuwzZccY6HX0y10csmS1rKM3s87xavXbvSN7VvlkTnbnMfn57fz27KK4vPr-4-zrZaFFJaYCUWFpLVesx7qqGOqmY1gaqI1gddWAAVbWzKABzbHpRM-EhFJVVaOslr04Jh-23vy3x9mkqQ0ubTaiBhPn1CKCAIn4X5ChrGshZQY_vg7W-SHIOGcZLbaoHmNKo7HtbnUtg3bTdPtP05k_3annLpj-L72rVjwD0wumxQ</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>Kuo, Y C</creator><creator>Meng, H C</creator><creator>Tsai, W J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20010201</creationdate><title>Regulation of cell proliferation, inflammatory cytokine production and calcium mobilization in primary human T lymphocytes by emodin from Polygonum hypoleucum Ohwi</title><author>Kuo, Y C ; Meng, H C ; Tsai, W J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-77a74ff2a1d765517c9b174e06e316590e01461e7e0c279b3d13804a559afc8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>anthraquinones</topic><topic>Calcium - metabolism</topic><topic>Cell Division - drug effects</topic><topic>Cytokines - biosynthesis</topic><topic>emodin</topic><topic>Emodin - administration & dosage</topic><topic>Emodin - pharmacology</topic><topic>Gene Expression - drug effects</topic><topic>Humans</topic><topic>Interleukin-2 - genetics</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Male</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>Plant Lectins</topic><topic>Polygonaceae - chemistry</topic><topic>Polygonum</topic><topic>Polygonum hypoleucum</topic><topic>RNA, Messenger - analysis</topic><topic>T-Lymphocytes - cytology</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuo, Y C</creatorcontrib><creatorcontrib>Meng, H C</creatorcontrib><creatorcontrib>Tsai, W J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Inflammation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuo, Y C</au><au>Meng, H C</au><au>Tsai, W J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of cell proliferation, inflammatory cytokine production and calcium mobilization in primary human T lymphocytes by emodin from Polygonum hypoleucum Ohwi</atitle><jtitle>Inflammation research</jtitle><addtitle>Inflamm Res</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>50</volume><issue>2</issue><spage>73</spage><epage>82</epage><pages>73-82</pages><issn>1023-3830</issn><eissn>1420-908X</eissn><abstract>This study was designed to elucidate action mechanisms of four anthraquinones identified from Polygonum hypoleucum Ohwi (P. hypoleucum Ohwi) on primary human T lymphocytes.
The cells were isolated from peripheral blood.
T cells were treated with 5 to 60 microM of four anthraquinones with or without phytohemagglutinin (PHA; 5 microg/ml) for 3 days. Effects of 4 anthraquinones on T lymphocyte proliferation, production and gene expression of inflammatory cytokines and intracellular free Ca2+ concentration ([Ca2+]i) were determined. Data were assessed with Student's t-test.
On a percentage basis, emodin had the highest suppressing activity on T lymphocyte proliferation with an IC50 of 11.2 +/- 0.6 microM. Emodin decreased cytokine production, IL-2 mRNA expression, and [Ca2+]i in activated T cells.
We hypothesize that the inhibitory mechanisms of emodin on activated T cells proliferation are related to the impairment of cytokine production, IL-2 mRNA level and [Ca2+]i in the cells.</abstract><cop>Switzerland</cop><pmid>11289657</pmid><doi>10.1007/s000110050727</doi><tpages>10</tpages></addata></record> |
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| ispartof | Inflammation research, 2001-02, Vol.50 (2), p.73-82 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adult anthraquinones Calcium - metabolism Cell Division - drug effects Cytokines - biosynthesis emodin Emodin - administration & dosage Emodin - pharmacology Gene Expression - drug effects Humans Interleukin-2 - genetics Lymphocyte Activation - drug effects Male Phytohemagglutinins - pharmacology Plant Lectins Polygonaceae - chemistry Polygonum Polygonum hypoleucum RNA, Messenger - analysis T-Lymphocytes - cytology T-Lymphocytes - drug effects T-Lymphocytes - metabolism |
| title | Regulation of cell proliferation, inflammatory cytokine production and calcium mobilization in primary human T lymphocytes by emodin from Polygonum hypoleucum Ohwi |
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