Amitotic Neuroblastoma Cells used for Neural Implants in Monkeys
The potential utility of cultured neuroblastoma cells as donor tissue for neural implants into the mammalian brain has been examined. Cells from a human neuroblastoma cell line, IMR-32, were labeled with [$^{3}$H]thymidine and chemically rendered amitotic. These differentiated IMR-32 cells were graf...
Gespeichert in:
| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1986-09, Vol.233 (4771), p.1420-1422 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1422 |
|---|---|
| container_issue | 4771 |
| container_start_page | 1420 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 233 |
| creator | Gash, D. M. Notter, M. F. D. Okawara, S. H. Kraus, A. L. Joynt, R. J. |
| description | The potential utility of cultured neuroblastoma cells as donor tissue for neural implants into the mammalian brain has been examined. Cells from a human neuroblastoma cell line, IMR-32, were labeled with [$^{3}$H]thymidine and chemically rendered amitotic. These differentiated IMR-32 cells were grafted into the hippocampi of five adult African Green monkeys, and graft survival was evaluated for up to 270 days after transplantation. Autoradiographically labeled grafted cells were identified in four animals. Processes from grafted cells could be followed for distances of up to 150 micrometers into the host brain. No evidence for neoplastic growth of the transplant was found. Thus, grafted neuroblastoma cells can survive for prolonged periods in the primate brain and may serve as a practical source of donor tissue for neural implants. |
| doi_str_mv | 10.1126/science.3749886 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_77026289</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A4503375</galeid><jstor_id>1697463</jstor_id><sourcerecordid>A4503375</sourcerecordid><originalsourceid>FETCH-LOGICAL-c730t-37f503ea1fe2a206b11c210b0f1842558fa83bd7b6276752e9782cb28d6820b23</originalsourceid><addsrcrecordid>eNqN081v0zAUAHALgUZXOHMBKUIIDiObPxLbuVEqKJXKeuDjGjnuS-WS2MVOpO2_x6XRNlClVj5Y8vv52Xr2Q-gFwZeEUH4VtAGr4ZKJrJCSP0Ijgos8LShmj9EIY8ZTiUX-FJ2HsME4xgp2hs4GPkIfJq3pXGd0cg29d1WjQudalUyhaULSB1gltfN_g6pJ5u22UbYLibHJV2d_wW14hp7UqgnwfJjH6MfnT9-nX9LFcjafThapFgx3KRN1jhkoUgNVFPOKEE0JrnBNZEbzXNZKsmolKk4FFzmFQkiqKypXXFJcUTZGb_d5t9797iF0ZWuCjrdUFlwfSiEw5VQWRyHjmcwZl0chJYThvDgOSRYz0mx39Ov_4Mb13sayxGQspyyLNRijiz1aqwZKY2vXeaXXYCFW2FmoTVye7CQTedTvD-g4VtAafYC_-4dH0cFNt1Z9COX82_WpcvnzVPlxdqKUs8VDeXFIatc0sIYy_pzp8qG-2mvtXQge6nLrTav8bUlwuWuDcmiDcvjXccer4SX6qoXVnb-PvxniKmjV1F5ZbcIdEwXnMW9kL_dsE7vC35_KC5Fxxv4A2WkTSA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213523450</pqid></control><display><type>article</type><title>Amitotic Neuroblastoma Cells used for Neural Implants in Monkeys</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>Science Magazine</source><creator>Gash, D. M. ; Notter, M. F. D. ; Okawara, S. H. ; Kraus, A. L. ; Joynt, R. J.</creator><creatorcontrib>Gash, D. M. ; Notter, M. F. D. ; Okawara, S. H. ; Kraus, A. L. ; Joynt, R. J.</creatorcontrib><description>The potential utility of cultured neuroblastoma cells as donor tissue for neural implants into the mammalian brain has been examined. Cells from a human neuroblastoma cell line, IMR-32, were labeled with [$^{3}$H]thymidine and chemically rendered amitotic. These differentiated IMR-32 cells were grafted into the hippocampi of five adult African Green monkeys, and graft survival was evaluated for up to 270 days after transplantation. Autoradiographically labeled grafted cells were identified in four animals. Processes from grafted cells could be followed for distances of up to 150 micrometers into the host brain. No evidence for neoplastic growth of the transplant was found. Thus, grafted neuroblastoma cells can survive for prolonged periods in the primate brain and may serve as a practical source of donor tissue for neural implants.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.3749886</identifier><identifier>PMID: 3749886</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: The American Association for the Advancement of Science</publisher><subject>Animals ; Autoradiography ; Biological and medical sciences ; Blood plasma ; Brain ; Brain - cytology ; Brain research ; Cell Line ; Cell lines ; Cellular differentiation ; Cercopithecus aethiops ; Cultured cells ; DNA Replication ; Female ; Humans ; Implants ; Male ; Medical research ; Medical sciences ; Neoplasm Transplantation ; Nervous system ; Nervous system involvement in other diseases. Miscellaneous ; Neuroblastoma ; Neuroblastoma - pathology ; Neurology ; Neurons ; Neurons - transplantation ; Pain ; Primates ; Thymidine - metabolism ; Tissue grafting ; Transplantation ; Tritium</subject><ispartof>Science (American Association for the Advancement of Science), 1986-09, Vol.233 (4771), p.1420-1422</ispartof><rights>Copyright 1986 The American Association for the Advancement of Science</rights><rights>1987 INIST-CNRS</rights><rights>COPYRIGHT 1986 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1986 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Sep 26, 1986</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c730t-37f503ea1fe2a206b11c210b0f1842558fa83bd7b6276752e9782cb28d6820b23</citedby><cites>FETCH-LOGICAL-c730t-37f503ea1fe2a206b11c210b0f1842558fa83bd7b6276752e9782cb28d6820b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1697463$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1697463$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7966112$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3749886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gash, D. M.</creatorcontrib><creatorcontrib>Notter, M. F. D.</creatorcontrib><creatorcontrib>Okawara, S. H.</creatorcontrib><creatorcontrib>Kraus, A. L.</creatorcontrib><creatorcontrib>Joynt, R. J.</creatorcontrib><title>Amitotic Neuroblastoma Cells used for Neural Implants in Monkeys</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The potential utility of cultured neuroblastoma cells as donor tissue for neural implants into the mammalian brain has been examined. Cells from a human neuroblastoma cell line, IMR-32, were labeled with [$^{3}$H]thymidine and chemically rendered amitotic. These differentiated IMR-32 cells were grafted into the hippocampi of five adult African Green monkeys, and graft survival was evaluated for up to 270 days after transplantation. Autoradiographically labeled grafted cells were identified in four animals. Processes from grafted cells could be followed for distances of up to 150 micrometers into the host brain. No evidence for neoplastic growth of the transplant was found. Thus, grafted neuroblastoma cells can survive for prolonged periods in the primate brain and may serve as a practical source of donor tissue for neural implants.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Blood plasma</subject><subject>Brain</subject><subject>Brain - cytology</subject><subject>Brain research</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cellular differentiation</subject><subject>Cercopithecus aethiops</subject><subject>Cultured cells</subject><subject>DNA Replication</subject><subject>Female</subject><subject>Humans</subject><subject>Implants</subject><subject>Male</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Neoplasm Transplantation</subject><subject>Nervous system</subject><subject>Nervous system involvement in other diseases. Miscellaneous</subject><subject>Neuroblastoma</subject><subject>Neuroblastoma - pathology</subject><subject>Neurology</subject><subject>Neurons</subject><subject>Neurons - transplantation</subject><subject>Pain</subject><subject>Primates</subject><subject>Thymidine - metabolism</subject><subject>Tissue grafting</subject><subject>Transplantation</subject><subject>Tritium</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN081v0zAUAHALgUZXOHMBKUIIDiObPxLbuVEqKJXKeuDjGjnuS-WS2MVOpO2_x6XRNlClVj5Y8vv52Xr2Q-gFwZeEUH4VtAGr4ZKJrJCSP0Ijgos8LShmj9EIY8ZTiUX-FJ2HsME4xgp2hs4GPkIfJq3pXGd0cg29d1WjQudalUyhaULSB1gltfN_g6pJ5u22UbYLibHJV2d_wW14hp7UqgnwfJjH6MfnT9-nX9LFcjafThapFgx3KRN1jhkoUgNVFPOKEE0JrnBNZEbzXNZKsmolKk4FFzmFQkiqKypXXFJcUTZGb_d5t9797iF0ZWuCjrdUFlwfSiEw5VQWRyHjmcwZl0chJYThvDgOSRYz0mx39Ov_4Mb13sayxGQspyyLNRijiz1aqwZKY2vXeaXXYCFW2FmoTVye7CQTedTvD-g4VtAafYC_-4dH0cFNt1Z9COX82_WpcvnzVPlxdqKUs8VDeXFIatc0sIYy_pzp8qG-2mvtXQge6nLrTav8bUlwuWuDcmiDcvjXccer4SX6qoXVnb-PvxniKmjV1F5ZbcIdEwXnMW9kL_dsE7vC35_KC5Fxxv4A2WkTSA</recordid><startdate>19860926</startdate><enddate>19860926</enddate><creator>Gash, D. M.</creator><creator>Notter, M. F. D.</creator><creator>Okawara, S. H.</creator><creator>Kraus, A. L.</creator><creator>Joynt, R. J.</creator><general>The American Association for the Advancement of Science</general><general>American Association for the Advancement of Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>IBG</scope><scope>IOV</scope><scope>ISN</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>19860926</creationdate><title>Amitotic Neuroblastoma Cells used for Neural Implants in Monkeys</title><author>Gash, D. M. ; Notter, M. F. D. ; Okawara, S. H. ; Kraus, A. L. ; Joynt, R. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c730t-37f503ea1fe2a206b11c210b0f1842558fa83bd7b6276752e9782cb28d6820b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Blood plasma</topic><topic>Brain</topic><topic>Brain - cytology</topic><topic>Brain research</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cellular differentiation</topic><topic>Cercopithecus aethiops</topic><topic>Cultured cells</topic><topic>DNA Replication</topic><topic>Female</topic><topic>Humans</topic><topic>Implants</topic><topic>Male</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Neoplasm Transplantation</topic><topic>Nervous system</topic><topic>Nervous system involvement in other diseases. Miscellaneous</topic><topic>Neuroblastoma</topic><topic>Neuroblastoma - pathology</topic><topic>Neurology</topic><topic>Neurons</topic><topic>Neurons - transplantation</topic><topic>Pain</topic><topic>Primates</topic><topic>Thymidine - metabolism</topic><topic>Tissue grafting</topic><topic>Transplantation</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gash, D. M.</creatorcontrib><creatorcontrib>Notter, M. F. D.</creatorcontrib><creatorcontrib>Okawara, S. H.</creatorcontrib><creatorcontrib>Kraus, A. L.</creatorcontrib><creatorcontrib>Joynt, R. J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: High School</collection><collection>Gale In Context: Biography</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Canada</collection><collection>Aluminium Industry Abstracts</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gash, D. M.</au><au>Notter, M. F. D.</au><au>Okawara, S. H.</au><au>Kraus, A. L.</au><au>Joynt, R. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amitotic Neuroblastoma Cells used for Neural Implants in Monkeys</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1986-09-26</date><risdate>1986</risdate><volume>233</volume><issue>4771</issue><spage>1420</spage><epage>1422</epage><pages>1420-1422</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>The potential utility of cultured neuroblastoma cells as donor tissue for neural implants into the mammalian brain has been examined. Cells from a human neuroblastoma cell line, IMR-32, were labeled with [$^{3}$H]thymidine and chemically rendered amitotic. These differentiated IMR-32 cells were grafted into the hippocampi of five adult African Green monkeys, and graft survival was evaluated for up to 270 days after transplantation. Autoradiographically labeled grafted cells were identified in four animals. Processes from grafted cells could be followed for distances of up to 150 micrometers into the host brain. No evidence for neoplastic growth of the transplant was found. Thus, grafted neuroblastoma cells can survive for prolonged periods in the primate brain and may serve as a practical source of donor tissue for neural implants.</abstract><cop>Washington, DC</cop><pub>The American Association for the Advancement of Science</pub><pmid>3749886</pmid><doi>10.1126/science.3749886</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1986-09, Vol.233 (4771), p.1420-1422 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77026289 |
| source | Jstor Complete Legacy; MEDLINE; Science Magazine |
| subjects | Animals Autoradiography Biological and medical sciences Blood plasma Brain Brain - cytology Brain research Cell Line Cell lines Cellular differentiation Cercopithecus aethiops Cultured cells DNA Replication Female Humans Implants Male Medical research Medical sciences Neoplasm Transplantation Nervous system Nervous system involvement in other diseases. Miscellaneous Neuroblastoma Neuroblastoma - pathology Neurology Neurons Neurons - transplantation Pain Primates Thymidine - metabolism Tissue grafting Transplantation Tritium |
| title | Amitotic Neuroblastoma Cells used for Neural Implants in Monkeys |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T22%3A07%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Amitotic%20Neuroblastoma%20Cells%20used%20for%20Neural%20Implants%20in%20Monkeys&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=Gash,%20D.%20M.&rft.date=1986-09-26&rft.volume=233&rft.issue=4771&rft.spage=1420&rft.epage=1422&rft.pages=1420-1422&rft.issn=0036-8075&rft.eissn=1095-9203&rft.coden=SCIEAS&rft_id=info:doi/10.1126/science.3749886&rft_dat=%3Cgale_proqu%3EA4503375%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=213523450&rft_id=info:pmid/3749886&rft_galeid=A4503375&rft_jstor_id=1697463&rfr_iscdi=true |