Quantitative determination of C-polysaccharide in Streptococcus pneumoniae capsular polysaccharides by use of high-performance anion-exchange chromatography with pulsed amperometric detection

Capsular polysaccharides of Streptococcus pneumoniae are used to formulate polyvalent pneumococcal vaccines. A sensitive method, using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), has been developed to quantify the contamination of pneumococcal capsu...

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Veröffentlicht in:	Vaccine 2001-04, Vol.19 (20), p.2987-2994
Hauptverfasser:	Talaga, Philippe, Bellamy, Laurent, Moreau, Monique
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Capsules - analysis Bacteriology Biological and medical sciences C-polysaccharide Capsular polysaccharides Chromatography, Ion Exchange Electrochemistry Fundamental and applied biological sciences. Psychology HPAEC-PAD hydrofluoric acid Hydrolysis Magnetic Resonance Spectroscopy Microbiology polysaccharides Streptococcus pneumoniae Streptococcus pneumoniae - chemistry trifluoroacetic acid Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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