Detection and quantification of the iap gene of Listeria monocytogenes and Listeria innocua by a new real-time quantitative PCR assay

A real-time quantitative polymerase chain reaction (PCR) assay for direct detection and enumeration of Listeria monocytogenes and Listeria innocua was developed and applied to artificially contaminated milk samples. The iap gene present in both species was used as a target for amplification of a 175...

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Veröffentlicht in:	Research in microbiology 2001-01, Vol.152 (1), p.37-46
Hauptverfasser:	Hein, Ingeborg, Klein, Dieter, Lehner, Angelika, Bubert, Andreas, Brandl, Ernst, Wagner, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	Aap gene Animals Bacterial Proteins - genetics Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Colony Count, Microbial Culture Media DNA Primers DNA, Bacterial - analysis DNA, Bacterial - isolation & purification Food industries Fundamental and applied biological sciences. Psychology Genes, Bacterial Listeria - genetics Listeria - isolation & purification Listeria innocua Listeria monocytogenes Listeria monocytogenes - genetics Listeria monocytogenes - growth & development Listeria monocytogenes - isolation & purification Microbiology milk Milk - microbiology Milk and cheese industries. Ice creams PCR Polymerase Chain Reaction - methods Sensitivity and Specificity
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