Effects of Fluence Rate on Cell Survival and Photobleaching in Meta-Tetra-(hydroxyphenyl)chlorin–photosensitized Colo 26 Multicell Tumor Spheroids
We report the influence of fluence rate on the photobleaching and cell survival in Colo 26 multicell spheroids photosensitized by meta-tetra-(hydroxyphenyl)chlorin (mTHPC). Photosensitizer degradation and therapeutic efficacy increased dramatically and progressively when the fluence rate was reduced...
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| Veröffentlicht in: | Photochemistry and photobiology 2001-03, Vol.73 (3), p.297-303 |
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| container_title | Photochemistry and photobiology |
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| creator | Coutier, Stéphanie Mitra, Soumya Bezdetnaya, Lina N. Parache, Robert M. Georgakoudi, Irene Foster, Thomas H. Guillemin, François |
| description | We report the influence of fluence rate on the photobleaching and cell survival in Colo 26 multicell spheroids photosensitized by meta-tetra-(hydroxyphenyl)chlorin (mTHPC). Photosensitizer degradation and therapeutic efficacy increased dramatically and progressively when the fluence rate was reduced over the range from 90 to 5 mW cm−2. These experimental results were compared to a mathematical model of photobleaching based on self-sensitized singlet oxygen reactions with the photosensitizer ground state. This model incorporates photophysical parameters obtained from microelectrode measurements of oxygen depletion at the surface of mTHPC-sensitized spheroids and was refined by including the inhomogeneous distribution of mTHPC in spheroids and oxygen depletion in the bulk medium. Since the model is consistent with the experimental data we conclude that the fluence rate dependence of the cell survival and of mTHPC photobleaching is due to photochemical oxygen consumption and a predominantly singlet oxygen-mediated mechanism of mTHPC photobleaching. The threshold dose of reacting singlet oxygen was calculated to be 7.9 ± 2.2 mM in this system. |
| doi_str_mv | 10.1562/0031-8655(2001)073<0297:EOFROC>2.0.CO;2 |
| format | Article |
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Photosensitizer degradation and therapeutic efficacy increased dramatically and progressively when the fluence rate was reduced over the range from 90 to 5 mW cm−2. These experimental results were compared to a mathematical model of photobleaching based on self-sensitized singlet oxygen reactions with the photosensitizer ground state. This model incorporates photophysical parameters obtained from microelectrode measurements of oxygen depletion at the surface of mTHPC-sensitized spheroids and was refined by including the inhomogeneous distribution of mTHPC in spheroids and oxygen depletion in the bulk medium. Since the model is consistent with the experimental data we conclude that the fluence rate dependence of the cell survival and of mTHPC photobleaching is due to photochemical oxygen consumption and a predominantly singlet oxygen-mediated mechanism of mTHPC photobleaching. The threshold dose of reacting singlet oxygen was calculated to be 7.9 ± 2.2 mM in this system.</description><identifier>ISSN: 0031-8655</identifier><identifier>EISSN: 1751-1097</identifier><identifier>DOI: 10.1562/0031-8655(2001)073<0297:EOFROC>2.0.CO;2</identifier><identifier>PMID: 11281027</identifier><identifier>CODEN: PHCBAP</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Animals ; Cell Survival - drug effects ; Cell Survival - radiation effects ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - pathology ; Mesoporphyrins - metabolism ; Mesoporphyrins - pharmacology ; Mice ; PHOTOMEDICINE ; Photosensitizing Agents - metabolism ; Photosensitizing Agents - pharmacology ; Tumor Cells, Cultured</subject><ispartof>Photochemistry and photobiology, 2001-03, Vol.73 (3), p.297-303</ispartof><rights>American Society for Photobiology</rights><rights>Copyright American Society of Photobiology Mar 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-b430t-e2d816030f7d913389f51f666759843656620a187c2203bba5541b7d7660dfe43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1562/0031-8655(2001)073<0297:EOFROC>2.0.CO;2$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,780,784,26978,27924,27925,52363</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11281027$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coutier, Stéphanie</creatorcontrib><creatorcontrib>Mitra, Soumya</creatorcontrib><creatorcontrib>Bezdetnaya, Lina N.</creatorcontrib><creatorcontrib>Parache, Robert M.</creatorcontrib><creatorcontrib>Georgakoudi, Irene</creatorcontrib><creatorcontrib>Foster, Thomas H.</creatorcontrib><creatorcontrib>Guillemin, François</creatorcontrib><title>Effects of Fluence Rate on Cell Survival and Photobleaching in Meta-Tetra-(hydroxyphenyl)chlorin–photosensitized Colo 26 Multicell Tumor Spheroids</title><title>Photochemistry and photobiology</title><addtitle>Photochem Photobiol</addtitle><description>We report the influence of fluence rate on the photobleaching and cell survival in Colo 26 multicell spheroids photosensitized by meta-tetra-(hydroxyphenyl)chlorin (mTHPC). Photosensitizer degradation and therapeutic efficacy increased dramatically and progressively when the fluence rate was reduced over the range from 90 to 5 mW cm−2. These experimental results were compared to a mathematical model of photobleaching based on self-sensitized singlet oxygen reactions with the photosensitizer ground state. This model incorporates photophysical parameters obtained from microelectrode measurements of oxygen depletion at the surface of mTHPC-sensitized spheroids and was refined by including the inhomogeneous distribution of mTHPC in spheroids and oxygen depletion in the bulk medium. Since the model is consistent with the experimental data we conclude that the fluence rate dependence of the cell survival and of mTHPC photobleaching is due to photochemical oxygen consumption and a predominantly singlet oxygen-mediated mechanism of mTHPC photobleaching. The threshold dose of reacting singlet oxygen was calculated to be 7.9 ± 2.2 mM in this system.</description><subject>Animals</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - radiation effects</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Mesoporphyrins - metabolism</subject><subject>Mesoporphyrins - pharmacology</subject><subject>Mice</subject><subject>PHOTOMEDICINE</subject><subject>Photosensitizing Agents - metabolism</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>0031-8655</issn><issn>1751-1097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqdkc1u1DAUhS0EosPAKyCLBWoXmfonsROoKqFopq3UKqgd1paTOMSVxx7spOp0xTvAE_IkOJoRSCxZ3c13zrnSB8ApRgucMXKKEMVJzrLsmCCETxCnZ4gU_MOyWt1W5TlZoEVZfSTPwAzzDCcYFfw5mP1JHYFXIdzHZFpw_BIcYUxyjAifgZ_LrlPNEKDr4MqMyjYK3spBQWdhqYyBd6N_0A_SQGlb-Ll3g6uNkk2v7VeoLbxRg0zWavAyOe53rXePu22v7M6cNL1xXttf339sp1RQNuhBP6kWls44SBi8Gc2gm2lkPW6ch3cx6Z1uw2vwopMmqDeHOwdfVst1eZlcVxdX5afrpE4pGhJF2hwzRFHH2wJTmhddhjvGGM-KPKUsY4wgiXPeEIJoXcssS3HNW84YajuV0jl4v-_devdtVGEQGx2mh6RVbgyCc4RjEkXw3T_gvRu9jb8JQjkuCp7TCF3soca7ELzqxNbrjfQ7gZGYLIrJh5h8iMmiiBbFZFHsLYoIiLKKjXPw9jA31hvV_u05aIvAcg_U2jmr_nvoNxoasPY</recordid><startdate>20010301</startdate><enddate>20010301</enddate><creator>Coutier, Stéphanie</creator><creator>Mitra, Soumya</creator><creator>Bezdetnaya, Lina N.</creator><creator>Parache, Robert M.</creator><creator>Georgakoudi, Irene</creator><creator>Foster, Thomas H.</creator><creator>Guillemin, François</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7RV</scope><scope>7TM</scope><scope>7U7</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20010301</creationdate><title>Effects of Fluence Rate on Cell Survival and Photobleaching in Meta-Tetra-(hydroxyphenyl)chlorin–photosensitized Colo 26 Multicell Tumor Spheroids</title><author>Coutier, Stéphanie ; Mitra, Soumya ; Bezdetnaya, Lina N. ; Parache, Robert M. ; Georgakoudi, Irene ; Foster, Thomas H. ; Guillemin, François</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b430t-e2d816030f7d913389f51f666759843656620a187c2203bba5541b7d7660dfe43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Cell Survival - 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Academic</collection><jtitle>Photochemistry and photobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coutier, Stéphanie</au><au>Mitra, Soumya</au><au>Bezdetnaya, Lina N.</au><au>Parache, Robert M.</au><au>Georgakoudi, Irene</au><au>Foster, Thomas H.</au><au>Guillemin, François</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Fluence Rate on Cell Survival and Photobleaching in Meta-Tetra-(hydroxyphenyl)chlorin–photosensitized Colo 26 Multicell Tumor Spheroids</atitle><jtitle>Photochemistry and photobiology</jtitle><addtitle>Photochem Photobiol</addtitle><date>2001-03-01</date><risdate>2001</risdate><volume>73</volume><issue>3</issue><spage>297</spage><epage>303</epage><pages>297-303</pages><issn>0031-8655</issn><eissn>1751-1097</eissn><coden>PHCBAP</coden><abstract>We report the influence of fluence rate on the photobleaching and cell survival in Colo 26 multicell spheroids photosensitized by meta-tetra-(hydroxyphenyl)chlorin (mTHPC). Photosensitizer degradation and therapeutic efficacy increased dramatically and progressively when the fluence rate was reduced over the range from 90 to 5 mW cm−2. These experimental results were compared to a mathematical model of photobleaching based on self-sensitized singlet oxygen reactions with the photosensitizer ground state. This model incorporates photophysical parameters obtained from microelectrode measurements of oxygen depletion at the surface of mTHPC-sensitized spheroids and was refined by including the inhomogeneous distribution of mTHPC in spheroids and oxygen depletion in the bulk medium. Since the model is consistent with the experimental data we conclude that the fluence rate dependence of the cell survival and of mTHPC photobleaching is due to photochemical oxygen consumption and a predominantly singlet oxygen-mediated mechanism of mTHPC photobleaching. The threshold dose of reacting singlet oxygen was calculated to be 7.9 ± 2.2 mM in this system.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>11281027</pmid><doi>10.1562/0031-8655(2001)073<0297:EOFROC>2.0.CO;2</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Cell Survival - drug effects Cell Survival - radiation effects Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Mesoporphyrins - metabolism Mesoporphyrins - pharmacology Mice PHOTOMEDICINE Photosensitizing Agents - metabolism Photosensitizing Agents - pharmacology Tumor Cells, Cultured |
| title | Effects of Fluence Rate on Cell Survival and Photobleaching in Meta-Tetra-(hydroxyphenyl)chlorin–photosensitized Colo 26 Multicell Tumor Spheroids |
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