Thermoprecipitation of lysozyme from egg white using copolymers of N-isopropylacrylamide and acidic monomers

Thermoprecipitation of lysozyme from egg white was demonstrated using copolymers of N-isopropylacrylamide with acrylic acid, methacrylic acid, 2-acryloylamido-2-methylpropane-sulfonic acid and itaconic acid, respectively. Polymers synthesized using molar feed ratio of N-isopropylacrylamide:acidic mo...

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Veröffentlicht in:	Journal of biotechnology 2001-05, Vol.87 (2), p.95-107
Hauptverfasser:	Vaidya, A.A, Lele, B.S, Kulkarni, M.G, Mashelkar, R.A
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosamine - chemistry Acidic monomers Acrylamides - chemistry Acrylates - chemistry acrylic acid Animals Binding Sites Biological and medical sciences Biotechnology Chemical Precipitation Egg White Enzyme engineering Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Improved methods for extraction and purification of enzymes Ions isopropylacrylamide Lysozyme Methacrylates - chemistry methacrylic acid Methods. Procedures. Technologies Muramidase - antagonists & inhibitors Muramidase - isolation & purification Muramidase - metabolism N-isopropylacrylamide Osmolar Concentration Polymers - chemistry Solutions Succinates - chemistry Thermoprecipitation Thermosensitive polymers
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