Measurement of potency and lipids in monensin fermentation broth by near-infrared spectroscopy
A transmission near-infrared (NIR) spectroscopic method for quantification of potency and lipids in monensin fermentation broth was developed and validated. Two multiple linear regression calibration curves were established for a set of 100 fermentation samples, correlating the appropriate absorptio...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2001-05, Vol.25 (2), p.239-256 |
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| creator | FORBES, Robert A LUO, Matthew Z SMITH, David R |
| description | A transmission near-infrared (NIR) spectroscopic method for quantification of potency and lipids in monensin fermentation broth was developed and validated. Two multiple linear regression calibration curves were established for a set of 100 fermentation samples, correlating the appropriate absorption bands in the NIR spectrum to the laboratory reference methods; high-performance liquid chromatography for potency, and chloroform extraction for lipids. During method development, potency was found to be well correlated to NIR absorbances specific for monensin. While acceptable, correlation of NIR absorbances characteristic of oil to the chloroform lipid method was weaker due to a greater amount of relative variation in the lipid measurements. Following establishment of the optimal calibration curves, the NIR method for potency and lipids was validated for selectivity, accuracy, precision, and robustness. In order to investigate long-term drift in the measurement system, samples were tested both by the NIR and the reference methods over a 7-month period. The differences between results from the two measurements were calculated and statistically analyzed. |
| doi_str_mv | 10.1016/S0731-7085(00)00497-0 |
| format | Article |
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Two multiple linear regression calibration curves were established for a set of 100 fermentation samples, correlating the appropriate absorption bands in the NIR spectrum to the laboratory reference methods; high-performance liquid chromatography for potency, and chloroform extraction for lipids. During method development, potency was found to be well correlated to NIR absorbances specific for monensin. While acceptable, correlation of NIR absorbances characteristic of oil to the chloroform lipid method was weaker due to a greater amount of relative variation in the lipid measurements. Following establishment of the optimal calibration curves, the NIR method for potency and lipids was validated for selectivity, accuracy, precision, and robustness. In order to investigate long-term drift in the measurement system, samples were tested both by the NIR and the reference methods over a 7-month period. The differences between results from the two measurements were calculated and statistically analyzed.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(00)00497-0</identifier><identifier>PMID: 11275433</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier Science</publisher><subject>Algorithms ; Analysis ; Biological and medical sciences ; Calibration ; Culture Media - analysis ; Fermentation ; General pharmacology ; Lipids - chemistry ; Medical sciences ; Models, Biological ; Monensin - biosynthesis ; Pharmacology. Drug treatments ; Reproducibility of Results ; Spectroscopy, Near-Infrared ; Streptomyces - chemistry ; Streptomyces - metabolism</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2001-05, Vol.25 (2), p.239-256</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c333t-eaa644220ab2ba4de81e6d09cbf188a6cb716d890a487a734821f1752886e8123</citedby><cites>FETCH-LOGICAL-c333t-eaa644220ab2ba4de81e6d09cbf188a6cb716d890a487a734821f1752886e8123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=938829$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11275433$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FORBES, Robert A</creatorcontrib><creatorcontrib>LUO, Matthew Z</creatorcontrib><creatorcontrib>SMITH, David R</creatorcontrib><title>Measurement of potency and lipids in monensin fermentation broth by near-infrared spectroscopy</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A transmission near-infrared (NIR) spectroscopic method for quantification of potency and lipids in monensin fermentation broth was developed and validated. Two multiple linear regression calibration curves were established for a set of 100 fermentation samples, correlating the appropriate absorption bands in the NIR spectrum to the laboratory reference methods; high-performance liquid chromatography for potency, and chloroform extraction for lipids. During method development, potency was found to be well correlated to NIR absorbances specific for monensin. While acceptable, correlation of NIR absorbances characteristic of oil to the chloroform lipid method was weaker due to a greater amount of relative variation in the lipid measurements. Following establishment of the optimal calibration curves, the NIR method for potency and lipids was validated for selectivity, accuracy, precision, and robustness. In order to investigate long-term drift in the measurement system, samples were tested both by the NIR and the reference methods over a 7-month period. The differences between results from the two measurements were calculated and statistically analyzed.</description><subject>Algorithms</subject><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Culture Media - analysis</subject><subject>Fermentation</subject><subject>General pharmacology</subject><subject>Lipids - chemistry</subject><subject>Medical sciences</subject><subject>Models, Biological</subject><subject>Monensin - biosynthesis</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Spectroscopy, Near-Infrared</subject><subject>Streptomyces - chemistry</subject><subject>Streptomyces - metabolism</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0E1P3DAQgGELFcEW-AlUlipV5RAYfyR2jgi1BQnEAZA4YU2ciUiV2KmdPey_JwsrerIPz4ytl7FTAecCRHXxAEaJwoAtfwKcAejaFLDHVsIaVchKP39hq09yyL7m_BcASlHrA3YohDSlVmrFXu4I8zrRSGHmseNTnCn4DcfQ8qGf-jbzPvAxBgp5uXSUthLnPgbepDi_8mbDA2Eq-tAlTNTyPJGfU8w-Tptjtt_hkOlkdx6xp9-_Hq-ui9v7PzdXl7eFV0rNBSFWWksJ2MgGdUtWUNVC7ZtOWIuVb4yoWlsDamvQKG2l6IQppbXVYqU6Yj8-9k4p_ltTnt3YZ0_DgIHiOjtjYMkA9QLLD-iXH-ZEnZtSP2LaOAFuG9a9h3Xbag7AvYd1sMx92z2wbkZq_0_tSi7g-w5g9jgsLYLv86erlbWyVm-dxIFp</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>FORBES, Robert A</creator><creator>LUO, Matthew Z</creator><creator>SMITH, David R</creator><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Measurement of potency and lipids in monensin fermentation broth by near-infrared spectroscopy</title><author>FORBES, Robert A ; LUO, Matthew Z ; SMITH, David R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-eaa644220ab2ba4de81e6d09cbf188a6cb716d890a487a734821f1752886e8123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Algorithms</topic><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Culture Media - analysis</topic><topic>Fermentation</topic><topic>General pharmacology</topic><topic>Lipids - chemistry</topic><topic>Medical sciences</topic><topic>Models, Biological</topic><topic>Monensin - biosynthesis</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Spectroscopy, Near-Infrared</topic><topic>Streptomyces - chemistry</topic><topic>Streptomyces - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FORBES, Robert A</creatorcontrib><creatorcontrib>LUO, Matthew Z</creatorcontrib><creatorcontrib>SMITH, David R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FORBES, Robert A</au><au>LUO, Matthew Z</au><au>SMITH, David R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of potency and lipids in monensin fermentation broth by near-infrared spectroscopy</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>25</volume><issue>2</issue><spage>239</spage><epage>256</epage><pages>239-256</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A transmission near-infrared (NIR) spectroscopic method for quantification of potency and lipids in monensin fermentation broth was developed and validated. Two multiple linear regression calibration curves were established for a set of 100 fermentation samples, correlating the appropriate absorption bands in the NIR spectrum to the laboratory reference methods; high-performance liquid chromatography for potency, and chloroform extraction for lipids. During method development, potency was found to be well correlated to NIR absorbances specific for monensin. While acceptable, correlation of NIR absorbances characteristic of oil to the chloroform lipid method was weaker due to a greater amount of relative variation in the lipid measurements. Following establishment of the optimal calibration curves, the NIR method for potency and lipids was validated for selectivity, accuracy, precision, and robustness. In order to investigate long-term drift in the measurement system, samples were tested both by the NIR and the reference methods over a 7-month period. The differences between results from the two measurements were calculated and statistically analyzed.</abstract><cop>Amsterdam</cop><pub>Elsevier Science</pub><pmid>11275433</pmid><doi>10.1016/S0731-7085(00)00497-0</doi><tpages>18</tpages></addata></record> |
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| ispartof | Journal of pharmaceutical and biomedical analysis, 2001-05, Vol.25 (2), p.239-256 |
| issn | 0731-7085 1873-264X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Algorithms Analysis Biological and medical sciences Calibration Culture Media - analysis Fermentation General pharmacology Lipids - chemistry Medical sciences Models, Biological Monensin - biosynthesis Pharmacology. Drug treatments Reproducibility of Results Spectroscopy, Near-Infrared Streptomyces - chemistry Streptomyces - metabolism |
| title | Measurement of potency and lipids in monensin fermentation broth by near-infrared spectroscopy |
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