Key peptide processing enzymes are expressed by breast cancer cells
The expression of the three key peptide processing enzyme families, represented by CPE, PAM, and PC1/3 plus PC2, were examined in MCF-7 and ZR-75-1 breast cancer cell lines. Both of these cell lines express vasopressin receptors as well as the vasopressin gene, but the processing of vasopressin gene...
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| Veröffentlicht in: | Cancer letters 2001-04, Vol.165 (2), p.211-218 |
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| creator | Du, Jinlin Keegan, Brendan P. North, William G. |
| description | The expression of the three key peptide processing enzyme families, represented by CPE, PAM, and PC1/3 plus PC2, were examined in MCF-7 and ZR-75-1 breast cancer cell lines. Both of these cell lines express vasopressin receptors as well as the vasopressin gene, but the processing of vasopressin gene-related proteins appears to be limited. Products of the expected size for, CPE, PAM and PC1/PC3 could be amplified by reverse transcription-polymerase chain reaction (RT-PCR) from both cell lines. Cloning and sequencing of these RT-PCR products revealed that each enzyme mRNA had a structure identical to that published for the human form of the respective enzyme. Western analysis provided evidence that mRNAs for these enzymes are translated into proteins. Alternatively, PC2 mRNA was identified to be present in MCF-7 cells both by RT-PCR and Western blot analysis, but could not be demonstrated for ZR-75-1 cells. Our findings suggest that the key processing enzymes needed to generate active vasopressin and other neuropeptide growth factors are present in breast cancer cells. |
| doi_str_mv | 10.1016/S0304-3835(01)00409-8 |
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Both of these cell lines express vasopressin receptors as well as the vasopressin gene, but the processing of vasopressin gene-related proteins appears to be limited. Products of the expected size for, CPE, PAM and PC1/PC3 could be amplified by reverse transcription-polymerase chain reaction (RT-PCR) from both cell lines. Cloning and sequencing of these RT-PCR products revealed that each enzyme mRNA had a structure identical to that published for the human form of the respective enzyme. Western analysis provided evidence that mRNAs for these enzymes are translated into proteins. Alternatively, PC2 mRNA was identified to be present in MCF-7 cells both by RT-PCR and Western blot analysis, but could not be demonstrated for ZR-75-1 cells. Our findings suggest that the key processing enzymes needed to generate active vasopressin and other neuropeptide growth factors are present in breast cancer cells.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/S0304-3835(01)00409-8</identifier><identifier>PMID: 11275371</identifier><identifier>CODEN: CALEDQ</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Biological and medical sciences ; Blotting, Western ; Breast cancer ; Breast Neoplasms - enzymology ; Carboxypeptidase E ; Carboxypeptidase H ; Carboxypeptidases - biosynthesis ; Cloning, Molecular ; Furin ; Gynecology. Andrology. Obstetrics ; Humans ; Mammary gland diseases ; Medical sciences ; Mixed Function Oxygenases - biosynthesis ; Multienzyme Complexes ; Neuropeptide ; Peptidylglycine α-amidating monooxygenase ; Processing ; Prohormone convertase ; Receptors, Vasopressin - biosynthesis ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Sequence Analysis, DNA ; Subtilisins - biosynthesis ; Tumor Cells, Cultured ; Tumors ; Vasopressin</subject><ispartof>Cancer letters, 2001-04, Vol.165 (2), p.211-218</ispartof><rights>2001 Elsevier Science Ireland Ltd</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-8cf59ee9408a01682df8ef65af4a35dd5e4c5efd6a64f533728048f95c65ee053</citedby><cites>FETCH-LOGICAL-c389t-8cf59ee9408a01682df8ef65af4a35dd5e4c5efd6a64f533728048f95c65ee053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304383501004098$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=987823$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11275371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Jinlin</creatorcontrib><creatorcontrib>Keegan, Brendan P.</creatorcontrib><creatorcontrib>North, William G.</creatorcontrib><title>Key peptide processing enzymes are expressed by breast cancer cells</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>The expression of the three key peptide processing enzyme families, represented by CPE, PAM, and PC1/3 plus PC2, were examined in MCF-7 and ZR-75-1 breast cancer cell lines. Both of these cell lines express vasopressin receptors as well as the vasopressin gene, but the processing of vasopressin gene-related proteins appears to be limited. Products of the expected size for, CPE, PAM and PC1/PC3 could be amplified by reverse transcription-polymerase chain reaction (RT-PCR) from both cell lines. Cloning and sequencing of these RT-PCR products revealed that each enzyme mRNA had a structure identical to that published for the human form of the respective enzyme. Western analysis provided evidence that mRNAs for these enzymes are translated into proteins. Alternatively, PC2 mRNA was identified to be present in MCF-7 cells both by RT-PCR and Western blot analysis, but could not be demonstrated for ZR-75-1 cells. Our findings suggest that the key processing enzymes needed to generate active vasopressin and other neuropeptide growth factors are present in breast cancer cells.</description><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - enzymology</subject><subject>Carboxypeptidase E</subject><subject>Carboxypeptidase H</subject><subject>Carboxypeptidases - biosynthesis</subject><subject>Cloning, Molecular</subject><subject>Furin</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Mixed Function Oxygenases - biosynthesis</subject><subject>Multienzyme Complexes</subject><subject>Neuropeptide</subject><subject>Peptidylglycine α-amidating monooxygenase</subject><subject>Processing</subject><subject>Prohormone convertase</subject><subject>Receptors, Vasopressin - biosynthesis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Subtilisins - biosynthesis</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Vasopressin</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotlZ_ghIQRA-rk81mkz2JFL9Q8KCeQ5pMJNKPNdmK9deb2lKPngaGZ2beeQg5ZHDOgNUXz8ChKrji4hTYGUAFTaG2SJ8pWRayUbBN-hukR_ZSegcAUUmxS3qMlVJwyfpk-IAL2mLbBYe0jTOLKYXpG8Xp92KCiZqIFL_amNvo6GhBRxFN6qg1U4uRWhyP0z7Z8Wac8GBdB-T15vpleFc8Pt3eD68eC8tV0xXKetEgNhUokz9QpfMKfS2MrwwXzgmsrEDvalNXXnAuSwWV8o2wtUAEwQfkZLU35_yYY-r0JKRlAjPF2TxpKQEkZ3UGxQq0cZZSRK_bGCYmLjQDvbSnf-3ppRoNTP_a0yrPHa0PzEcTdH9Ta10ZOF4DJlkz9jFbCGnDNUqqkmfqckVhlvEZMOpkA2ZfLkS0nXaz8E-QH0K1i0g</recordid><startdate>20010426</startdate><enddate>20010426</enddate><creator>Du, Jinlin</creator><creator>Keegan, Brendan P.</creator><creator>North, William G.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010426</creationdate><title>Key peptide processing enzymes are expressed by breast cancer cells</title><author>Du, Jinlin ; Keegan, Brendan P. ; North, William G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-8cf59ee9408a01682df8ef65af4a35dd5e4c5efd6a64f533728048f95c65ee053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - enzymology</topic><topic>Carboxypeptidase E</topic><topic>Carboxypeptidase H</topic><topic>Carboxypeptidases - biosynthesis</topic><topic>Cloning, Molecular</topic><topic>Furin</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>Mixed Function Oxygenases - biosynthesis</topic><topic>Multienzyme Complexes</topic><topic>Neuropeptide</topic><topic>Peptidylglycine α-amidating monooxygenase</topic><topic>Processing</topic><topic>Prohormone convertase</topic><topic>Receptors, Vasopressin - biosynthesis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Subtilisins - biosynthesis</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><topic>Vasopressin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Jinlin</creatorcontrib><creatorcontrib>Keegan, Brendan P.</creatorcontrib><creatorcontrib>North, William G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Jinlin</au><au>Keegan, Brendan P.</au><au>North, William G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Key peptide processing enzymes are expressed by breast cancer cells</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2001-04-26</date><risdate>2001</risdate><volume>165</volume><issue>2</issue><spage>211</spage><epage>218</epage><pages>211-218</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><coden>CALEDQ</coden><abstract>The expression of the three key peptide processing enzyme families, represented by CPE, PAM, and PC1/3 plus PC2, were examined in MCF-7 and ZR-75-1 breast cancer cell lines. Both of these cell lines express vasopressin receptors as well as the vasopressin gene, but the processing of vasopressin gene-related proteins appears to be limited. Products of the expected size for, CPE, PAM and PC1/PC3 could be amplified by reverse transcription-polymerase chain reaction (RT-PCR) from both cell lines. Cloning and sequencing of these RT-PCR products revealed that each enzyme mRNA had a structure identical to that published for the human form of the respective enzyme. Western analysis provided evidence that mRNAs for these enzymes are translated into proteins. Alternatively, PC2 mRNA was identified to be present in MCF-7 cells both by RT-PCR and Western blot analysis, but could not be demonstrated for ZR-75-1 cells. Our findings suggest that the key processing enzymes needed to generate active vasopressin and other neuropeptide growth factors are present in breast cancer cells.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>11275371</pmid><doi>10.1016/S0304-3835(01)00409-8</doi><tpages>8</tpages></addata></record> |
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| subjects | Biological and medical sciences Blotting, Western Breast cancer Breast Neoplasms - enzymology Carboxypeptidase E Carboxypeptidase H Carboxypeptidases - biosynthesis Cloning, Molecular Furin Gynecology. Andrology. Obstetrics Humans Mammary gland diseases Medical sciences Mixed Function Oxygenases - biosynthesis Multienzyme Complexes Neuropeptide Peptidylglycine α-amidating monooxygenase Processing Prohormone convertase Receptors, Vasopressin - biosynthesis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sequence Analysis, DNA Subtilisins - biosynthesis Tumor Cells, Cultured Tumors Vasopressin |
| title | Key peptide processing enzymes are expressed by breast cancer cells |
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