The persistent infection of a canine thymus cell line by equine infectious anaemia virus and preliminary data on the production of viral antigens
Equine infectious anaemia virus (EIAV) was adapted to the Cf2Th cell line, a heterologous malignant line from canine thymus. A persistent infection was monitored for 100 serial passages by demonstrating the presence of virus and viral antigens at each 10th passage by electron-microscopy, immunodiffu...
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| Veröffentlicht in: | Journal of virological methods 1986-07, Vol.13 (4), p.309-321 |
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| container_title | Journal of virological methods |
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| creator | Bouillant, A.M.P. Nielsen, K. Ruckerbauer, G.M. Samagh, B.S. Hare, W.C.D. |
| description | Equine infectious anaemia virus (EIAV) was adapted to the Cf2Th cell line, a heterologous malignant line from canine thymus. A persistent infection was monitored for 100 serial passages by demonstrating the presence of virus and viral antigens at each 10th passage by electron-microscopy, immunodiffusion and immunofluorescence. Chromosome analysis of EIAV-infected cells indicated they had a karyotype resembling the control cells of similar passage history. Virus-infected cells, grown in roller cultures for 65 days without subculturing, continuously produced viral antigens into supernatant fluids which were harvested every 3–4 days. Antigen peaks were observed at approximately 12-day intervals. Immunoprecipitin lines of identity were demonstrated between ether-extracted antigens from virus-infected canine cell line and known positive EIAV antigen extracted from infected equine spleen and a commercial source. Replication of a non-oncogenic retrovirus (EIAV) resulted in the continuous release of viral antigens from a persistently infected and infinite cell line. |
| doi_str_mv | 10.1016/0166-0934(86)90056-X |
| format | Article |
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A persistent infection was monitored for 100 serial passages by demonstrating the presence of virus and viral antigens at each 10th passage by electron-microscopy, immunodiffusion and immunofluorescence. Chromosome analysis of EIAV-infected cells indicated they had a karyotype resembling the control cells of similar passage history. Virus-infected cells, grown in roller cultures for 65 days without subculturing, continuously produced viral antigens into supernatant fluids which were harvested every 3–4 days. Antigen peaks were observed at approximately 12-day intervals. Immunoprecipitin lines of identity were demonstrated between ether-extracted antigens from virus-infected canine cell line and known positive EIAV antigen extracted from infected equine spleen and a commercial source. 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Psychology ; Horses ; Immunodiffusion ; Infectious Anemia Virus, Equine - growth & development ; Infectious Anemia Virus, Equine - immunology ; Infectious Anemia Virus, Equine - ultrastructure ; Microbiology ; Microscopy, Electron ; Techniques used in virology ; Thymus Gland ; tumor cells ; Virology</subject><ispartof>Journal of virological methods, 1986-07, Vol.13 (4), p.309-321</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-4508da82252a6bd338a707209c6e1d5301f1f81ebffd50c4987c20596dad9ea33</citedby><cites>FETCH-LOGICAL-c386t-4508da82252a6bd338a707209c6e1d5301f1f81ebffd50c4987c20596dad9ea33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/016609348690056X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8063866$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3018020$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bouillant, A.M.P.</creatorcontrib><creatorcontrib>Nielsen, K.</creatorcontrib><creatorcontrib>Ruckerbauer, G.M.</creatorcontrib><creatorcontrib>Samagh, B.S.</creatorcontrib><creatorcontrib>Hare, W.C.D.</creatorcontrib><title>The persistent infection of a canine thymus cell line by equine infectious anaemia virus and preliminary data on the production of viral antigens</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>Equine infectious anaemia virus (EIAV) was adapted to the Cf2Th cell line, a heterologous malignant line from canine thymus. A persistent infection was monitored for 100 serial passages by demonstrating the presence of virus and viral antigens at each 10th passage by electron-microscopy, immunodiffusion and immunofluorescence. Chromosome analysis of EIAV-infected cells indicated they had a karyotype resembling the control cells of similar passage history. Virus-infected cells, grown in roller cultures for 65 days without subculturing, continuously produced viral antigens into supernatant fluids which were harvested every 3–4 days. Antigen peaks were observed at approximately 12-day intervals. Immunoprecipitin lines of identity were demonstrated between ether-extracted antigens from virus-infected canine cell line and known positive EIAV antigen extracted from infected equine spleen and a commercial source. Replication of a non-oncogenic retrovirus (EIAV) resulted in the continuous release of viral antigens from a persistently infected and infinite cell line.</description><subject>Animals</subject><subject>antigen production in vitro</subject><subject>Antigens, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Dogs</subject><subject>equine infectious anaemia virus</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Horses</subject><subject>Immunodiffusion</subject><subject>Infectious Anemia Virus, Equine - growth & development</subject><subject>Infectious Anemia Virus, Equine - immunology</subject><subject>Infectious Anemia Virus, Equine - ultrastructure</subject><subject>Microbiology</subject><subject>Microscopy, Electron</subject><subject>Techniques used in virology</subject><subject>Thymus Gland</subject><subject>tumor cells</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc-KFDEQxoMo6-zqGyjkIKKH1kr_SacvgiyuCgteVthbqEkqbqQ7PZukF-YxfGPTO-McPYQkVb-q5PuKsVcCPggQ8mNZsoKhad8p-X4A6GR1-4RthOqHElbtU7Y5Ic_ZeUq_oUB905yxswaEgho27M_NHfEdxeRTppC5D45M9nPgs-PIDQYfiOe7_bQkbmgc-bgGtntO98t6-ldQ0hiQJo_8wcfHm-W7SKOffMC45xYz8tI3rw_G2S6nZwqPY-Gz_0UhvWDPHI6JXh73C_bz6svN5bfq-sfX75efryvTKJmrtgNlUdV1V6Pc2qZR2ENfw2AkCdsVgU44JWjrnO3AtIPqTQ3dIC3agbBpLtjbQ9_ymfuFUtaTT6tCDFTU6L4HkKLrCtgeQBPnlCI5vYt-KpK0AL1OQq8269VmraR-nIS-LWWvj_2X7UT2VHS0vuTfHPOYDI4uYjA-nTAFsuiUBft0wKh48eAp6mQ8BUPWx2K8trP__z_-AsvYp1o</recordid><startdate>19860701</startdate><enddate>19860701</enddate><creator>Bouillant, A.M.P.</creator><creator>Nielsen, K.</creator><creator>Ruckerbauer, G.M.</creator><creator>Samagh, B.S.</creator><creator>Hare, W.C.D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860701</creationdate><title>The persistent infection of a canine thymus cell line by equine infectious anaemia virus and preliminary data on the production of viral antigens</title><author>Bouillant, A.M.P. ; Nielsen, K. ; Ruckerbauer, G.M. ; Samagh, B.S. ; Hare, W.C.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-4508da82252a6bd338a707209c6e1d5301f1f81ebffd50c4987c20596dad9ea33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>antigen production in vitro</topic><topic>Antigens, Viral - analysis</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Dogs</topic><topic>equine infectious anaemia virus</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Horses</topic><topic>Immunodiffusion</topic><topic>Infectious Anemia Virus, Equine - growth & development</topic><topic>Infectious Anemia Virus, Equine - immunology</topic><topic>Infectious Anemia Virus, Equine - ultrastructure</topic><topic>Microbiology</topic><topic>Microscopy, Electron</topic><topic>Techniques used in virology</topic><topic>Thymus Gland</topic><topic>tumor cells</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bouillant, A.M.P.</creatorcontrib><creatorcontrib>Nielsen, K.</creatorcontrib><creatorcontrib>Ruckerbauer, G.M.</creatorcontrib><creatorcontrib>Samagh, B.S.</creatorcontrib><creatorcontrib>Hare, W.C.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bouillant, A.M.P.</au><au>Nielsen, K.</au><au>Ruckerbauer, G.M.</au><au>Samagh, B.S.</au><au>Hare, W.C.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The persistent infection of a canine thymus cell line by equine infectious anaemia virus and preliminary data on the production of viral antigens</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1986-07-01</date><risdate>1986</risdate><volume>13</volume><issue>4</issue><spage>309</spage><epage>321</epage><pages>309-321</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>Equine infectious anaemia virus (EIAV) was adapted to the Cf2Th cell line, a heterologous malignant line from canine thymus. A persistent infection was monitored for 100 serial passages by demonstrating the presence of virus and viral antigens at each 10th passage by electron-microscopy, immunodiffusion and immunofluorescence. Chromosome analysis of EIAV-infected cells indicated they had a karyotype resembling the control cells of similar passage history. Virus-infected cells, grown in roller cultures for 65 days without subculturing, continuously produced viral antigens into supernatant fluids which were harvested every 3–4 days. Antigen peaks were observed at approximately 12-day intervals. Immunoprecipitin lines of identity were demonstrated between ether-extracted antigens from virus-infected canine cell line and known positive EIAV antigen extracted from infected equine spleen and a commercial source. Replication of a non-oncogenic retrovirus (EIAV) resulted in the continuous release of viral antigens from a persistently infected and infinite cell line.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3018020</pmid><doi>10.1016/0166-0934(86)90056-X</doi><tpages>13</tpages></addata></record> |
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| ispartof | Journal of virological methods, 1986-07, Vol.13 (4), p.309-321 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals antigen production in vitro Antigens, Viral - analysis Biological and medical sciences Cell Line Dogs equine infectious anaemia virus Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Horses Immunodiffusion Infectious Anemia Virus, Equine - growth & development Infectious Anemia Virus, Equine - immunology Infectious Anemia Virus, Equine - ultrastructure Microbiology Microscopy, Electron Techniques used in virology Thymus Gland tumor cells Virology |
| title | The persistent infection of a canine thymus cell line by equine infectious anaemia virus and preliminary data on the production of viral antigens |
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