Purification and some properties of component A of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS

Pseudomonas sp. strain CBS 3 possesses a two-component enzyme system which converts 4-chlorophenylacetate to 3,4-dihydroxyphenylacetate by the incorporation of 2 atoms of molecular oxygen. Component A of this enzyme system was purified to homogeneity by a 5-step procedure. After the last purificatio...

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Veröffentlicht in:	The Journal of biological chemistry 1986-09, Vol.261 (27), p.12883-12888
Hauptverfasser:	Markus, A, Krekel, D, Lingens, F
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange Dioxygenases Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Isoelectric Point Molecular Weight Oxidoreductases Oxygenases - isolation & purification Pseudomonas - enzymology
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