Phylogenetic investigation for the risk of hepatitis C virus transmission to surgical and dental patients
Blood loss during treatment carries a potential risk for the transmission of blood‐borne pathogens in hospital patients. To determine whether nosocomial transmission of hepatitis C virus (HCV) occurs in surgical wards and dental hospitals, we tested anti‐HCV antibodies and HCV RNA in sera from these...
Gespeichert in:
| Veröffentlicht in: | Journal of viral hepatitis 2001-03, Vol.8 (2), p.148-153 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 153 |
|---|---|
| container_issue | 2 |
| container_start_page | 148 |
| container_title | Journal of viral hepatitis |
| container_volume | 8 |
| creator | Enomoto, A. Yoshino, S. Hasegawa, H. Komatsu, T. Sasahara, H. Takano, S. Esumi, M. |
| description | Blood loss during treatment carries a potential risk for the transmission of blood‐borne pathogens in hospital patients. To determine whether nosocomial transmission of hepatitis C virus (HCV) occurs in surgical wards and dental hospitals, we tested anti‐HCV antibodies and HCV RNA in sera from these patients and analysed the hypervariable region 1 (HVR1) sequence of HCV phylogenetically in the HCV RNA‐positive patients. Five of 83 patients from a surgical ward were positive for HCV RNA, and six patients from one dental hospital and nine patients from a second were found to be positive for HCV RNA during the examination period. The HVR1 sequences were amplified from these patients’ serum, and after subcloning, multiple clones of the HVR1 sequence from each patient were determined. The phylogenetic analysis of these sequences showed that HVR1 species from each patient could be classified into one to three genetic clusters of HVR1 quasi‐species and that these clusters were independent of each other among patients. Thus, there was no evidence of HCV transmission in our study, and unrecognized transmission of HCV may be a rare event in surgical and dental patients at university hospitals. |
| doi_str_mv | 10.1046/j.1365-2893.2001.00261.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77003512</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17879343</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4331-30b621a540997509ab3c2fde8932befa711d555b05eb24cfc13dc833dd46be4a3</originalsourceid><addsrcrecordid>eNqNkUFv1DAQhS0EomXhLyCfuCV47DhOJC5ogRZUFQ6FIi6W40x2vc0mi-2U3X-Pw67KEU5-0rxvPDOPEAosB1aUrzc5iFJmvKpFzhmDnDFeQr5_RM4fCo9nLXnGJCvOyLMQNskouISn5AyAl4US8py4L-tDP65wwOgsdcM9huhWJrpxoN3oaVwj9S7c0bGja9ylQnSBLum981Og0ZshbF0Isz2ONEx-5azpqRla2uIQk5yZpMJz8qQzfcAXp3dBvn54f7O8zK4-X3xcvr3KbCEEZII1JQcjC1bXSrLaNMLyrsW0EW-wMwqglVI2TGLDC9tZEK2thGjbomywMGJBXh377vz4c0rr6DSgxb43A45T0EoxJiTwfxpBVaoWaagFqY5G68cQPHZ6593W-IMGpuc89EbPZ9fz2fWch_6Th94n9OXpj6nZYvsXPAWQDG-Ohl-ux8N_N9afvl0mkfDsiLsQcf-AG3-nSyWU1LfXFxrkbfXj3c21_i5-A_cWqdo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17879343</pqid></control><display><type>article</type><title>Phylogenetic investigation for the risk of hepatitis C virus transmission to surgical and dental patients</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Enomoto, A. ; Yoshino, S. ; Hasegawa, H. ; Komatsu, T. ; Sasahara, H. ; Takano, S. ; Esumi, M.</creator><creatorcontrib>Enomoto, A. ; Yoshino, S. ; Hasegawa, H. ; Komatsu, T. ; Sasahara, H. ; Takano, S. ; Esumi, M.</creatorcontrib><description>Blood loss during treatment carries a potential risk for the transmission of blood‐borne pathogens in hospital patients. To determine whether nosocomial transmission of hepatitis C virus (HCV) occurs in surgical wards and dental hospitals, we tested anti‐HCV antibodies and HCV RNA in sera from these patients and analysed the hypervariable region 1 (HVR1) sequence of HCV phylogenetically in the HCV RNA‐positive patients. Five of 83 patients from a surgical ward were positive for HCV RNA, and six patients from one dental hospital and nine patients from a second were found to be positive for HCV RNA during the examination period. The HVR1 sequences were amplified from these patients’ serum, and after subcloning, multiple clones of the HVR1 sequence from each patient were determined. The phylogenetic analysis of these sequences showed that HVR1 species from each patient could be classified into one to three genetic clusters of HVR1 quasi‐species and that these clusters were independent of each other among patients. Thus, there was no evidence of HCV transmission in our study, and unrecognized transmission of HCV may be a rare event in surgical and dental patients at university hospitals.</description><identifier>ISSN: 1352-0504</identifier><identifier>EISSN: 1365-2893</identifier><identifier>DOI: 10.1046/j.1365-2893.2001.00261.x</identifier><identifier>PMID: 11264735</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>Adult ; Aged ; Amino Acid Sequence ; dental hospital ; Dental Service, Hospital ; Disease Transmission, Infectious ; Female ; Hepacivirus - chemistry ; Hepacivirus - genetics ; Hepatitis C - blood ; Hepatitis C - transmission ; Hepatitis C - virology ; Hepatitis C Antibodies - blood ; Hepatitis C virus ; Humans ; hypervariable region 1 ; Japan ; Male ; Middle Aged ; Molecular Sequence Data ; nosocomial transmission ; phylogenetic analysis ; Phylogeny ; Risk Factors ; RNA, Viral - analysis ; Sequence Alignment ; Surgery Department, Hospital ; surgical ward ; Viral Proteins - classification ; Viral Proteins - genetics</subject><ispartof>Journal of viral hepatitis, 2001-03, Vol.8 (2), p.148-153</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4331-30b621a540997509ab3c2fde8932befa711d555b05eb24cfc13dc833dd46be4a3</citedby><cites>FETCH-LOGICAL-c4331-30b621a540997509ab3c2fde8932befa711d555b05eb24cfc13dc833dd46be4a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2893.2001.00261.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2893.2001.00261.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11264735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Enomoto, A.</creatorcontrib><creatorcontrib>Yoshino, S.</creatorcontrib><creatorcontrib>Hasegawa, H.</creatorcontrib><creatorcontrib>Komatsu, T.</creatorcontrib><creatorcontrib>Sasahara, H.</creatorcontrib><creatorcontrib>Takano, S.</creatorcontrib><creatorcontrib>Esumi, M.</creatorcontrib><title>Phylogenetic investigation for the risk of hepatitis C virus transmission to surgical and dental patients</title><title>Journal of viral hepatitis</title><addtitle>J Viral Hepat</addtitle><description>Blood loss during treatment carries a potential risk for the transmission of blood‐borne pathogens in hospital patients. To determine whether nosocomial transmission of hepatitis C virus (HCV) occurs in surgical wards and dental hospitals, we tested anti‐HCV antibodies and HCV RNA in sera from these patients and analysed the hypervariable region 1 (HVR1) sequence of HCV phylogenetically in the HCV RNA‐positive patients. Five of 83 patients from a surgical ward were positive for HCV RNA, and six patients from one dental hospital and nine patients from a second were found to be positive for HCV RNA during the examination period. The HVR1 sequences were amplified from these patients’ serum, and after subcloning, multiple clones of the HVR1 sequence from each patient were determined. The phylogenetic analysis of these sequences showed that HVR1 species from each patient could be classified into one to three genetic clusters of HVR1 quasi‐species and that these clusters were independent of each other among patients. Thus, there was no evidence of HCV transmission in our study, and unrecognized transmission of HCV may be a rare event in surgical and dental patients at university hospitals.</description><subject>Adult</subject><subject>Aged</subject><subject>Amino Acid Sequence</subject><subject>dental hospital</subject><subject>Dental Service, Hospital</subject><subject>Disease Transmission, Infectious</subject><subject>Female</subject><subject>Hepacivirus - chemistry</subject><subject>Hepacivirus - genetics</subject><subject>Hepatitis C - blood</subject><subject>Hepatitis C - transmission</subject><subject>Hepatitis C - virology</subject><subject>Hepatitis C Antibodies - blood</subject><subject>Hepatitis C virus</subject><subject>Humans</subject><subject>hypervariable region 1</subject><subject>Japan</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>nosocomial transmission</subject><subject>phylogenetic analysis</subject><subject>Phylogeny</subject><subject>Risk Factors</subject><subject>RNA, Viral - analysis</subject><subject>Sequence Alignment</subject><subject>Surgery Department, Hospital</subject><subject>surgical ward</subject><subject>Viral Proteins - classification</subject><subject>Viral Proteins - genetics</subject><issn>1352-0504</issn><issn>1365-2893</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS0EomXhLyCfuCV47DhOJC5ogRZUFQ6FIi6W40x2vc0mi-2U3X-Pw67KEU5-0rxvPDOPEAosB1aUrzc5iFJmvKpFzhmDnDFeQr5_RM4fCo9nLXnGJCvOyLMQNskouISn5AyAl4US8py4L-tDP65wwOgsdcM9huhWJrpxoN3oaVwj9S7c0bGja9ylQnSBLum981Og0ZshbF0Isz2ONEx-5azpqRla2uIQk5yZpMJz8qQzfcAXp3dBvn54f7O8zK4-X3xcvr3KbCEEZII1JQcjC1bXSrLaNMLyrsW0EW-wMwqglVI2TGLDC9tZEK2thGjbomywMGJBXh377vz4c0rr6DSgxb43A45T0EoxJiTwfxpBVaoWaagFqY5G68cQPHZ6593W-IMGpuc89EbPZ9fz2fWch_6Th94n9OXpj6nZYvsXPAWQDG-Ohl-ux8N_N9afvl0mkfDsiLsQcf-AG3-nSyWU1LfXFxrkbfXj3c21_i5-A_cWqdo</recordid><startdate>200103</startdate><enddate>200103</enddate><creator>Enomoto, A.</creator><creator>Yoshino, S.</creator><creator>Hasegawa, H.</creator><creator>Komatsu, T.</creator><creator>Sasahara, H.</creator><creator>Takano, S.</creator><creator>Esumi, M.</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200103</creationdate><title>Phylogenetic investigation for the risk of hepatitis C virus transmission to surgical and dental patients</title><author>Enomoto, A. ; Yoshino, S. ; Hasegawa, H. ; Komatsu, T. ; Sasahara, H. ; Takano, S. ; Esumi, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4331-30b621a540997509ab3c2fde8932befa711d555b05eb24cfc13dc833dd46be4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Amino Acid Sequence</topic><topic>dental hospital</topic><topic>Dental Service, Hospital</topic><topic>Disease Transmission, Infectious</topic><topic>Female</topic><topic>Hepacivirus - chemistry</topic><topic>Hepacivirus - genetics</topic><topic>Hepatitis C - blood</topic><topic>Hepatitis C - transmission</topic><topic>Hepatitis C - virology</topic><topic>Hepatitis C Antibodies - blood</topic><topic>Hepatitis C virus</topic><topic>Humans</topic><topic>hypervariable region 1</topic><topic>Japan</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>nosocomial transmission</topic><topic>phylogenetic analysis</topic><topic>Phylogeny</topic><topic>Risk Factors</topic><topic>RNA, Viral - analysis</topic><topic>Sequence Alignment</topic><topic>Surgery Department, Hospital</topic><topic>surgical ward</topic><topic>Viral Proteins - classification</topic><topic>Viral Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Enomoto, A.</creatorcontrib><creatorcontrib>Yoshino, S.</creatorcontrib><creatorcontrib>Hasegawa, H.</creatorcontrib><creatorcontrib>Komatsu, T.</creatorcontrib><creatorcontrib>Sasahara, H.</creatorcontrib><creatorcontrib>Takano, S.</creatorcontrib><creatorcontrib>Esumi, M.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of viral hepatitis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Enomoto, A.</au><au>Yoshino, S.</au><au>Hasegawa, H.</au><au>Komatsu, T.</au><au>Sasahara, H.</au><au>Takano, S.</au><au>Esumi, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic investigation for the risk of hepatitis C virus transmission to surgical and dental patients</atitle><jtitle>Journal of viral hepatitis</jtitle><addtitle>J Viral Hepat</addtitle><date>2001-03</date><risdate>2001</risdate><volume>8</volume><issue>2</issue><spage>148</spage><epage>153</epage><pages>148-153</pages><issn>1352-0504</issn><eissn>1365-2893</eissn><abstract>Blood loss during treatment carries a potential risk for the transmission of blood‐borne pathogens in hospital patients. To determine whether nosocomial transmission of hepatitis C virus (HCV) occurs in surgical wards and dental hospitals, we tested anti‐HCV antibodies and HCV RNA in sera from these patients and analysed the hypervariable region 1 (HVR1) sequence of HCV phylogenetically in the HCV RNA‐positive patients. Five of 83 patients from a surgical ward were positive for HCV RNA, and six patients from one dental hospital and nine patients from a second were found to be positive for HCV RNA during the examination period. The HVR1 sequences were amplified from these patients’ serum, and after subcloning, multiple clones of the HVR1 sequence from each patient were determined. The phylogenetic analysis of these sequences showed that HVR1 species from each patient could be classified into one to three genetic clusters of HVR1 quasi‐species and that these clusters were independent of each other among patients. Thus, there was no evidence of HCV transmission in our study, and unrecognized transmission of HCV may be a rare event in surgical and dental patients at university hospitals.</abstract><cop>Oxford UK</cop><pub>Blackwell Science Ltd</pub><pmid>11264735</pmid><doi>10.1046/j.1365-2893.2001.00261.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1352-0504 |
| ispartof | Journal of viral hepatitis, 2001-03, Vol.8 (2), p.148-153 |
| issn | 1352-0504 1365-2893 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77003512 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Adult Aged Amino Acid Sequence dental hospital Dental Service, Hospital Disease Transmission, Infectious Female Hepacivirus - chemistry Hepacivirus - genetics Hepatitis C - blood Hepatitis C - transmission Hepatitis C - virology Hepatitis C Antibodies - blood Hepatitis C virus Humans hypervariable region 1 Japan Male Middle Aged Molecular Sequence Data nosocomial transmission phylogenetic analysis Phylogeny Risk Factors RNA, Viral - analysis Sequence Alignment Surgery Department, Hospital surgical ward Viral Proteins - classification Viral Proteins - genetics |
| title | Phylogenetic investigation for the risk of hepatitis C virus transmission to surgical and dental patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T15%3A36%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phylogenetic%20investigation%20for%20the%20risk%20of%20hepatitis%20C%20virus%20transmission%20to%20surgical%20and%20dental%20patients&rft.jtitle=Journal%20of%20viral%20hepatitis&rft.au=Enomoto,%20A.&rft.date=2001-03&rft.volume=8&rft.issue=2&rft.spage=148&rft.epage=153&rft.pages=148-153&rft.issn=1352-0504&rft.eissn=1365-2893&rft_id=info:doi/10.1046/j.1365-2893.2001.00261.x&rft_dat=%3Cproquest_cross%3E17879343%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17879343&rft_id=info:pmid/11264735&rfr_iscdi=true |