Rapid Prenatal Diagnosis and Exclusion of Epidermolysis Bullosa Using Novel Antibody Probes
Prenatal diagnosis of recessive dystrophic epidermolysis bullosa was successfully achieved at 19 weeks' gestation by indirect immunofluorescence examination of a fetal skin biopsy sample using the monoclonal antibody LH 7:2. The abortus displayed marked blistering and the diagnosis was confirme...
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| Veröffentlicht in: | Journal of investigative dermatology 1986-05, Vol.86 (5), p.603-605 |
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| container_end_page | 605 |
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| container_issue | 5 |
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| container_title | Journal of investigative dermatology |
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| creator | Heagerty, Adrian H M Kennedy, Andrew R Gunner, David B Eady, Robin A J |
| description | Prenatal diagnosis of recessive dystrophic epidermolysis bullosa was successfully achieved at 19 weeks' gestation by indirect immunofluorescence examination of a fetal skin biopsy sample using the monoclonal antibody LH 7:2. The abortus displayed marked blistering and the diagnosis was confirmed by transmission electron microscopy (TEM). In 3 further pregnancies at risk for lethal junctional epidermolysis bullosa the diagnosis was excluded using the polyclonal antibody AA3. In all these studies the results were available within 4h of receiving the samples. These new techniques offer a quick and simple alternative to TEM for midtrimester prenatal diagnosis of 2 severe recessive forms of epidermolysis bullosa. |
| doi_str_mv | 10.1111/1523-1747.ep12355579 |
| format | Article |
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The abortus displayed marked blistering and the diagnosis was confirmed by transmission electron microscopy (TEM). In 3 further pregnancies at risk for lethal junctional epidermolysis bullosa the diagnosis was excluded using the polyclonal antibody AA3. In all these studies the results were available within 4h of receiving the samples. These new techniques offer a quick and simple alternative to TEM for midtrimester prenatal diagnosis of 2 severe recessive forms of epidermolysis bullosa.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep12355579</identifier><identifier>PMID: 3528313</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>Abortion, Therapeutic ; Adult ; Antibodies ; Antibodies, Monoclonal ; Biological and medical sciences ; Bullous diseases of the skin ; Dermatology ; Diagnosis, Differential ; Epidermis - ultrastructure ; Epidermolysis Bullosa - pathology ; Extraembryonic Membranes - ultrastructure ; Female ; Fluorescent Antibody Technique ; Humans ; Medical sciences ; Pregnancy ; Prenatal Diagnosis</subject><ispartof>Journal of investigative dermatology, 1986-05, Vol.86 (5), p.603-605</ispartof><rights>1986 The Society for Investigative Dermatology, Inc</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-6c1dd442cf77487df9cdc530c99f58af8bde729fdf5708fad2a6282a5e2d660e3</citedby><cites>FETCH-LOGICAL-c505t-6c1dd442cf77487df9cdc530c99f58af8bde729fdf5708fad2a6282a5e2d660e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8680914$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3528313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heagerty, Adrian H M</creatorcontrib><creatorcontrib>Kennedy, Andrew R</creatorcontrib><creatorcontrib>Gunner, David B</creatorcontrib><creatorcontrib>Eady, Robin A J</creatorcontrib><title>Rapid Prenatal Diagnosis and Exclusion of Epidermolysis Bullosa Using Novel Antibody Probes</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Prenatal diagnosis of recessive dystrophic epidermolysis bullosa was successfully achieved at 19 weeks' gestation by indirect immunofluorescence examination of a fetal skin biopsy sample using the monoclonal antibody LH 7:2. The abortus displayed marked blistering and the diagnosis was confirmed by transmission electron microscopy (TEM). In 3 further pregnancies at risk for lethal junctional epidermolysis bullosa the diagnosis was excluded using the polyclonal antibody AA3. In all these studies the results were available within 4h of receiving the samples. These new techniques offer a quick and simple alternative to TEM for midtrimester prenatal diagnosis of 2 severe recessive forms of epidermolysis bullosa.</description><subject>Abortion, Therapeutic</subject><subject>Adult</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Bullous diseases of the skin</subject><subject>Dermatology</subject><subject>Diagnosis, Differential</subject><subject>Epidermis - ultrastructure</subject><subject>Epidermolysis Bullosa - pathology</subject><subject>Extraembryonic Membranes - ultrastructure</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Pregnancy</subject><subject>Prenatal Diagnosis</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rVDEUhoModVr9BwpZiLtb83Fzk7sRap2qUFTEguAiZJKTEskk0-Te4vx7M84w3Wk2gTzveTk8QegFJee0nTdUMN5R2ctz2FDGhRByfIQWx-fHaEEIYx0j7MdTdFrrL0Lo0At1gk64YIpTvkA_v5lNcPhrgWQmE_H7YG5TrqFikxxe_rZxriEnnD1etiCUdY7bHX43x5irwTc1pFv8Od9DxBdpCqvstq0ur6A-Q0-8iRWeH-4zdHO1_H75sbv-8uHT5cV1ZwURUzdY6lzfM-ul7JV0frTOCk7sOHqhjFcrB5KN3nkhifLGMTMwxYwA5oaBAD9Dr_e9m5LvZqiTXodqIUaTIM9VS0kI53z8b5D2A2dE8hbs90Fbcq0FvN6UsDZlqynRO_l6Z1nvLOsH-W3s5aF_Xq3BHYcOtht_deCmWhN9McmGeoypQZGR9g817UvmAkcuhgb_8rd7Dk3qfYCiqw2QLLhQwE7a5fDvPf8AW6mrBQ</recordid><startdate>19860501</startdate><enddate>19860501</enddate><creator>Heagerty, Adrian H M</creator><creator>Kennedy, Andrew R</creator><creator>Gunner, David B</creator><creator>Eady, Robin A J</creator><general>Elsevier Inc</general><general>Nature Publishing</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19860501</creationdate><title>Rapid Prenatal Diagnosis and Exclusion of Epidermolysis Bullosa Using Novel Antibody Probes</title><author>Heagerty, Adrian H M ; Kennedy, Andrew R ; Gunner, David B ; Eady, Robin A J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-6c1dd442cf77487df9cdc530c99f58af8bde729fdf5708fad2a6282a5e2d660e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Abortion, Therapeutic</topic><topic>Adult</topic><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Bullous diseases of the skin</topic><topic>Dermatology</topic><topic>Diagnosis, Differential</topic><topic>Epidermis - ultrastructure</topic><topic>Epidermolysis Bullosa - pathology</topic><topic>Extraembryonic Membranes - ultrastructure</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Pregnancy</topic><topic>Prenatal Diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heagerty, Adrian H M</creatorcontrib><creatorcontrib>Kennedy, Andrew R</creatorcontrib><creatorcontrib>Gunner, David B</creatorcontrib><creatorcontrib>Eady, Robin A J</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heagerty, Adrian H M</au><au>Kennedy, Andrew R</au><au>Gunner, David B</au><au>Eady, Robin A J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Prenatal Diagnosis and Exclusion of Epidermolysis Bullosa Using Novel Antibody Probes</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1986-05-01</date><risdate>1986</risdate><volume>86</volume><issue>5</issue><spage>603</spage><epage>605</epage><pages>603-605</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>Prenatal diagnosis of recessive dystrophic epidermolysis bullosa was successfully achieved at 19 weeks' gestation by indirect immunofluorescence examination of a fetal skin biopsy sample using the monoclonal antibody LH 7:2. The abortus displayed marked blistering and the diagnosis was confirmed by transmission electron microscopy (TEM). In 3 further pregnancies at risk for lethal junctional epidermolysis bullosa the diagnosis was excluded using the polyclonal antibody AA3. In all these studies the results were available within 4h of receiving the samples. These new techniques offer a quick and simple alternative to TEM for midtrimester prenatal diagnosis of 2 severe recessive forms of epidermolysis bullosa.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>3528313</pmid><doi>10.1111/1523-1747.ep12355579</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of investigative dermatology, 1986-05, Vol.86 (5), p.603-605 |
| issn | 0022-202X 1523-1747 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Abortion, Therapeutic Adult Antibodies Antibodies, Monoclonal Biological and medical sciences Bullous diseases of the skin Dermatology Diagnosis, Differential Epidermis - ultrastructure Epidermolysis Bullosa - pathology Extraembryonic Membranes - ultrastructure Female Fluorescent Antibody Technique Humans Medical sciences Pregnancy Prenatal Diagnosis |
| title | Rapid Prenatal Diagnosis and Exclusion of Epidermolysis Bullosa Using Novel Antibody Probes |
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