Purification and characterization of a senile amyloid-related antigenic substance (apoSASSAM) from mouse serum. apoSASSAM is an apoA-II apolipoprotein of mouse high density lipoproteins

Two putative serum precursors which cross-react with antiserum against murine senile amyloid protein (ASSAM) were isolated from the high density lipoprotein (HDL) of normal mouse serum. Apolipoproteins designated "apoSASSAM-1" and "apoSASSAM-2" have the same molecular weight as t...

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Veröffentlicht in:The Journal of biological chemistry 1986-09, Vol.261 (27), p.12834-12840
Hauptverfasser: Higuchi, K, Yonezu, T, Kogishi, K, Matsumura, A, Takeshita, S, Kohno, A, Matsushita, M, Hosokawa, M, Takeda, T
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container_end_page 12840
container_issue 27
container_start_page 12834
container_title The Journal of biological chemistry
container_volume 261
creator Higuchi, K
Yonezu, T
Kogishi, K
Matsumura, A
Takeshita, S
Kohno, A
Matsushita, M
Hosokawa, M
Takeda, T
description Two putative serum precursors which cross-react with antiserum against murine senile amyloid protein (ASSAM) were isolated from the high density lipoprotein (HDL) of normal mouse serum. Apolipoproteins designated "apoSASSAM-1" and "apoSASSAM-2" have the same molecular weight as tissue amyloid fibril protein. ApoSASSAM-1 and apoSASSAM-2 migrate to an intermediate position between apoA-I and apoC on alkaline-urea polyacrylamide gel electrophoresis and are present mainly in HDL apoproteins and to a slight extent in very low density lipoprotein apoproteins when compared to apoC. ApoSASSAM-1 and apoSASSAM-2 are polymorphic; there are two apparent isoproteins of apoSASSAM-1 with isoelectric points of 4.72 and 4.79 and two major isoproteins of apo-SASSAM-2. Subunit bands of ASSAM separated by alkaline-urea polyacrylamide gel electrophoresis and that migrated to the same positions as apoSASSAM-1 and apoSASSAM-2 were labeled by anti-apoSASSAM-1 antiserum. The amino acid compositions of apoSASSAM-1 and apoSASSAM-2 were much the same and closely resembled those of ASSAM and mouse apoA-II. Sequence analysis of apoSASSAM and ASSAM revealed a blocked amino terminus. ApoSASSAM is considered to be a mouse apoA-II and probably transforms to amyloid fibril "ASSAM" in tissues through a process yet to be clarified.
doi_str_mv 10.1016/S0021-9258(18)67168-6
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ApoSASSAM is considered to be a mouse apoA-II and probably transforms to amyloid fibril "ASSAM" in tissues through a process yet to be clarified.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>3091606</pmid><doi>10.1016/S0021-9258(18)67168-6</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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ispartof The Journal of biological chemistry, 1986-09, Vol.261 (27), p.12834-12840
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source MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Amino Acids - analysis
Analytical, structural and metabolic biochemistry
Animals
Apolipoprotein A-II
Apolipoproteins - isolation & purification
Apolipoproteins A - blood
Biological and medical sciences
Chromatography, Gel
Fundamental and applied biological sciences. Psychology
Immunodiffusion
Isoelectric Focusing
Lipoproteins, HDL - blood
Lipoproteins, myelin
Mice
Molecular Weight
Proteins
title Purification and characterization of a senile amyloid-related antigenic substance (apoSASSAM) from mouse serum. apoSASSAM is an apoA-II apolipoprotein of mouse high density lipoproteins
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