Immunofractionation of DNA sequences associated with HMG-17 in chromatin
Antibodies specific for chromosomal protein HMG-17 were immobilized on CNBr-Sepharose and the resulting immunoaffinity column was used to purify chromatin segments enriched in HMG-17. The DNA was purified from both the nucleosomal fraction which was bound to the column and from the fraction which wa...
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| Veröffentlicht in: | Experimental cell research 1986-10, Vol.166 (2), p.486-496 |
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| container_title | Experimental cell research |
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| creator | Druckmann, Shulamith Mendelson, Ella Landsman, David Bustin, Michael |
| description | Antibodies specific for chromosomal protein HMG-17 were immobilized on CNBr-Sepharose and the resulting immunoaffinity column was used to purify chromatin segments enriched in HMG-17. The DNA was purified from both the nucleosomal fraction which was bound to the column and from the fraction which was not bound, and examined with DNA probes representing repetitive DNA, non-transcribed genes, transcribed genes and inducible genes. The results suggest that HMG-17 is preferentially associated with DNA sequences coding for genes, regardless of whether they are transcribed, and therefore support the notion that HMG-17 confers specific structural characteristics on selected regions in the genome. |
| doi_str_mv | 10.1016/0014-4827(86)90493-3 |
| format | Article |
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Genome</subject><subject>High Mobility Group Proteins - analysis</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Nucleosomes - analysis</subject><subject>Rats</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Transcription, Genetic</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EglL4A5CyQAgWAY_t-rFBqsqjlXhsYG05jiOMmhjsBMTfk9CqS9jMLObc0cxB6AjwBWDglxgDy5kk4kzyc4WZojndQiPACueEEbKNRhtkD-2n9IYxlhL4LtqlglHO5QjNF3XdNaGKxrY-NGYoWaiy68dpltxH5xrrUmZSCtab1pXZl29fs_nDXQ4i801mX2Oo-1RzgHYqs0zucN3H6OX25nk2z--f7haz6X1uGYg2t2pSMEepcEqZCQdbQUFlIQujSiASSCUFrwRYIq20VChDQRBuJC0mBDNOx-h0tfc9hv681OraJ-uWS9O40CUtuFJKYvgXBDbhQijcg2wF2hhSiq7S79HXJn5rwHowrQeNetCoJde_pjXtY8fr_V1Ru3ITWqvt5yfruUnWLHvDjfVpg8n-LaoG7GqFuV7ap3dRJ-sH66WPzra6DP7vO34ALBWXnA</recordid><startdate>19861001</startdate><enddate>19861001</enddate><creator>Druckmann, Shulamith</creator><creator>Mendelson, Ella</creator><creator>Landsman, David</creator><creator>Bustin, Michael</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19861001</creationdate><title>Immunofractionation of DNA sequences associated with HMG-17 in chromatin</title><author>Druckmann, Shulamith ; Mendelson, Ella ; Landsman, David ; Bustin, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-c95b4e337e99a561cf1b38b8ba9d12812f876f71c28c8c379a31726a83b520463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatin - analysis</topic><topic>DNA - analysis</topic><topic>DNA - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>High Mobility Group Proteins - analysis</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nucleosomes - analysis</topic><topic>Rats</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Druckmann, Shulamith</creatorcontrib><creatorcontrib>Mendelson, Ella</creatorcontrib><creatorcontrib>Landsman, David</creatorcontrib><creatorcontrib>Bustin, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Druckmann, Shulamith</au><au>Mendelson, Ella</au><au>Landsman, David</au><au>Bustin, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunofractionation of DNA sequences associated with HMG-17 in chromatin</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1986-10-01</date><risdate>1986</risdate><volume>166</volume><issue>2</issue><spage>486</spage><epage>496</epage><pages>486-496</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>Antibodies specific for chromosomal protein HMG-17 were immobilized on CNBr-Sepharose and the resulting immunoaffinity column was used to purify chromatin segments enriched in HMG-17. 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| ispartof | Experimental cell research, 1986-10, Vol.166 (2), p.486-496 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Chromatin - analysis DNA - analysis DNA - genetics Fundamental and applied biological sciences. Psychology Genes Genes. Genome High Mobility Group Proteins - analysis Molecular and cellular biology Molecular genetics Nucleosomes - analysis Rats Repetitive Sequences, Nucleic Acid Transcription, Genetic |
| title | Immunofractionation of DNA sequences associated with HMG-17 in chromatin |
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