Efficient gene transduction by RGD-fiber modified recombinant adenovirus into dendritic cells
Dendritic cells (DC) are important antigen-presenting cells in the development of an anti-tumor T cell response. To extend the range of current immuno / gene therapies, we tested luciferase-expressing RGD-adenovirus (Ad) (Ad5lucRGD)-mediated transduction into DC. Phenotypically characterized DC were...
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| Veröffentlicht in: | Japanese journal of cancer research (Gann) 2001-03, Vol.92 (3), p.321-327 |
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| creator | ASADA-MIKAMI, Rumiko HEIKE, Yuji WAKASUGI, Hiro KANAI, Sachiyo AZUMA, Masato SHIRAKAWA, Kazuo TAKAUE, Yoichi KRASNYKH, Victor CURIEL, David T TERADA, Masaaki ABE, Tatsuo |
| description | Dendritic cells (DC) are important antigen-presenting cells in the development of an anti-tumor T cell response. To extend the range of current immuno / gene therapies, we tested luciferase-expressing RGD-adenovirus (Ad) (Ad5lucRGD)-mediated transduction into DC. Phenotypically characterized DC were generated from peripheral blood CD14(+) cells by incubation with granulocyte-macrophage colony-stimulating factor, interleukin-4 and tumor necrosis factor alpha. On the 7th day of culture, the cells became mature DC with a CD1a(+), CD11c(+), CD80(+), CD83(+), CD86(+), human leukocyte antigen (HLA)-DR(+), CD14- phenotype. The expression of alpha( v)beta(3) integrin was enhanced on day 3 and returned to the basal level on day 7. We then compared the transduction efficiency of an Ad5lucRGD system to that using conventional Ad, in cells harvested on days 1, 3 and 7 of culture. Luciferase activity was negligible in AdCMVLuc, but remarkable in cells processed with Ad5lucRGD. Activity was maximal in cells that had been cultured for 3 days. Recombinant Ad5 fiber knob protein blocked AdCMVLuc- and Ad5lucRGD-mediated gene transduction by 90% and 20%, respectively. Surface markers and cytokine production were not affected by Ad5lucRGD-mediated transduction. |
| doi_str_mv | 10.1111/j.1349-7006.2001.tb01098.x |
| format | Article |
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To extend the range of current immuno / gene therapies, we tested luciferase-expressing RGD-adenovirus (Ad) (Ad5lucRGD)-mediated transduction into DC. Phenotypically characterized DC were generated from peripheral blood CD14(+) cells by incubation with granulocyte-macrophage colony-stimulating factor, interleukin-4 and tumor necrosis factor alpha. On the 7th day of culture, the cells became mature DC with a CD1a(+), CD11c(+), CD80(+), CD83(+), CD86(+), human leukocyte antigen (HLA)-DR(+), CD14- phenotype. The expression of alpha( v)beta(3) integrin was enhanced on day 3 and returned to the basal level on day 7. We then compared the transduction efficiency of an Ad5lucRGD system to that using conventional Ad, in cells harvested on days 1, 3 and 7 of culture. Luciferase activity was negligible in AdCMVLuc, but remarkable in cells processed with Ad5lucRGD. Activity was maximal in cells that had been cultured for 3 days. Recombinant Ad5 fiber knob protein blocked AdCMVLuc- and Ad5lucRGD-mediated gene transduction by 90% and 20%, respectively. Surface markers and cytokine production were not affected by Ad5lucRGD-mediated transduction.</description><identifier>ISSN: 0910-5050</identifier><identifier>EISSN: 1876-4673</identifier><identifier>DOI: 10.1111/j.1349-7006.2001.tb01098.x</identifier><identifier>PMID: 11267943</identifier><identifier>CODEN: GANNA2</identifier><language>eng</language><publisher>Tokyo: Japanese Cancer Association</publisher><subject>Adenoviridae - genetics ; Amino Acid Sequence ; Antigens, CD - analysis ; Biological and medical sciences ; Biotechnology ; Cell Culture Techniques - methods ; Cells, Cultured ; Dendritic Cells - cytology ; Dendritic Cells - drug effects ; Dendritic Cells - immunology ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Gene therapy ; Genes, Reporter ; Genetic Vectors ; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology ; Health. Pharmaceutical industry ; Humans ; Immunophenotyping ; Industrial applications and implications. Economical aspects ; Integrins - analysis ; Interleukin-4 - pharmacology ; Lipopolysaccharide Receptors - analysis ; Luciferases - analysis ; Luciferases - genetics ; Lung Neoplasms ; Oligopeptides ; Transfection - methods ; Tumor Cells, Cultured ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Japanese journal of cancer research (Gann), 2001-03, Vol.92 (3), p.321-327</ispartof><rights>2001 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1121728$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11267943$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ASADA-MIKAMI, Rumiko</creatorcontrib><creatorcontrib>HEIKE, Yuji</creatorcontrib><creatorcontrib>WAKASUGI, Hiro</creatorcontrib><creatorcontrib>KANAI, Sachiyo</creatorcontrib><creatorcontrib>AZUMA, Masato</creatorcontrib><creatorcontrib>SHIRAKAWA, Kazuo</creatorcontrib><creatorcontrib>TAKAUE, Yoichi</creatorcontrib><creatorcontrib>KRASNYKH, Victor</creatorcontrib><creatorcontrib>CURIEL, David T</creatorcontrib><creatorcontrib>TERADA, Masaaki</creatorcontrib><creatorcontrib>ABE, Tatsuo</creatorcontrib><title>Efficient gene transduction by RGD-fiber modified recombinant adenovirus into dendritic cells</title><title>Japanese journal of cancer research (Gann)</title><addtitle>Jpn J Cancer Res</addtitle><description>Dendritic cells (DC) are important antigen-presenting cells in the development of an anti-tumor T cell response. To extend the range of current immuno / gene therapies, we tested luciferase-expressing RGD-adenovirus (Ad) (Ad5lucRGD)-mediated transduction into DC. Phenotypically characterized DC were generated from peripheral blood CD14(+) cells by incubation with granulocyte-macrophage colony-stimulating factor, interleukin-4 and tumor necrosis factor alpha. On the 7th day of culture, the cells became mature DC with a CD1a(+), CD11c(+), CD80(+), CD83(+), CD86(+), human leukocyte antigen (HLA)-DR(+), CD14- phenotype. The expression of alpha( v)beta(3) integrin was enhanced on day 3 and returned to the basal level on day 7. We then compared the transduction efficiency of an Ad5lucRGD system to that using conventional Ad, in cells harvested on days 1, 3 and 7 of culture. Luciferase activity was negligible in AdCMVLuc, but remarkable in cells processed with Ad5lucRGD. Activity was maximal in cells that had been cultured for 3 days. Recombinant Ad5 fiber knob protein blocked AdCMVLuc- and Ad5lucRGD-mediated gene transduction by 90% and 20%, respectively. Surface markers and cytokine production were not affected by Ad5lucRGD-mediated transduction.</description><subject>Adenoviridae - genetics</subject><subject>Amino Acid Sequence</subject><subject>Antigens, CD - analysis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - methods</subject><subject>Cells, Cultured</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - drug effects</subject><subject>Dendritic Cells - immunology</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene therapy</subject><subject>Genes, Reporter</subject><subject>Genetic Vectors</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>Health. Pharmaceutical industry</subject><subject>Humans</subject><subject>Immunophenotyping</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Integrins - analysis</subject><subject>Interleukin-4 - pharmacology</subject><subject>Lipopolysaccharide Receptors - analysis</subject><subject>Luciferases - analysis</subject><subject>Luciferases - genetics</subject><subject>Lung Neoplasms</subject><subject>Oligopeptides</subject><subject>Transfection - methods</subject><subject>Tumor Cells, Cultured</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0910-5050</issn><issn>1876-4673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0NtKxDAQBuAgiruuvoIEEe9aM0mbNJei6yosCKKXUnKULD2sSSvu21txBedmGPhmGH6ELoDkMNX1JgdWyEwQwnNKCOSDJkBklX8doDlUgmcFF-wQzYkEkpWkJDN0ktJmooJweoxmAJQLWbA5elt6H0xw3YDfXefwEFWX7GiG0HdY7_Dz6i7zQbuI294GH5zF0Zm-1aFT046yrus_QxwTDt3Q42m0MQzBYOOaJp2iI6-a5M72fYFe75cvtw_Z-mn1eHuzzja0kkNGLS0pU1zJUngvia7AMsHBGGJBAGjliZZgNRecUpClk0oXjtqSFM4Uli3Q1e_dbew_RpeGug3p5wPVuX5MteBSlpKxCZ7v4ahbZ-ttDK2Ku_ovkAlc7oFKRjV-isOE9N-BoBX7Bj1ycpY</recordid><startdate>20010301</startdate><enddate>20010301</enddate><creator>ASADA-MIKAMI, Rumiko</creator><creator>HEIKE, Yuji</creator><creator>WAKASUGI, Hiro</creator><creator>KANAI, Sachiyo</creator><creator>AZUMA, Masato</creator><creator>SHIRAKAWA, Kazuo</creator><creator>TAKAUE, Yoichi</creator><creator>KRASNYKH, Victor</creator><creator>CURIEL, David T</creator><creator>TERADA, Masaaki</creator><creator>ABE, Tatsuo</creator><general>Japanese Cancer Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20010301</creationdate><title>Efficient gene transduction by RGD-fiber modified recombinant adenovirus into dendritic cells</title><author>ASADA-MIKAMI, Rumiko ; HEIKE, Yuji ; WAKASUGI, Hiro ; KANAI, Sachiyo ; AZUMA, Masato ; SHIRAKAWA, Kazuo ; TAKAUE, Yoichi ; KRASNYKH, Victor ; CURIEL, David T ; TERADA, Masaaki ; ABE, Tatsuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j289t-2d2523a6a957ff90b81d3761cc0d1711baf0b91db67622195e9ab4e2d504ec4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adenoviridae - genetics</topic><topic>Amino Acid Sequence</topic><topic>Antigens, CD - analysis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques - methods</topic><topic>Cells, Cultured</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - drug effects</topic><topic>Dendritic Cells - immunology</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene therapy</topic><topic>Genes, Reporter</topic><topic>Genetic Vectors</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>Health. Pharmaceutical industry</topic><topic>Humans</topic><topic>Immunophenotyping</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Integrins - analysis</topic><topic>Interleukin-4 - pharmacology</topic><topic>Lipopolysaccharide Receptors - analysis</topic><topic>Luciferases - analysis</topic><topic>Luciferases - genetics</topic><topic>Lung Neoplasms</topic><topic>Oligopeptides</topic><topic>Transfection - methods</topic><topic>Tumor Cells, Cultured</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>online_resources</toplevel><creatorcontrib>ASADA-MIKAMI, Rumiko</creatorcontrib><creatorcontrib>HEIKE, Yuji</creatorcontrib><creatorcontrib>WAKASUGI, Hiro</creatorcontrib><creatorcontrib>KANAI, Sachiyo</creatorcontrib><creatorcontrib>AZUMA, Masato</creatorcontrib><creatorcontrib>SHIRAKAWA, Kazuo</creatorcontrib><creatorcontrib>TAKAUE, Yoichi</creatorcontrib><creatorcontrib>KRASNYKH, Victor</creatorcontrib><creatorcontrib>CURIEL, David T</creatorcontrib><creatorcontrib>TERADA, Masaaki</creatorcontrib><creatorcontrib>ABE, Tatsuo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese journal of cancer research (Gann)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ASADA-MIKAMI, Rumiko</au><au>HEIKE, Yuji</au><au>WAKASUGI, Hiro</au><au>KANAI, Sachiyo</au><au>AZUMA, Masato</au><au>SHIRAKAWA, Kazuo</au><au>TAKAUE, Yoichi</au><au>KRASNYKH, Victor</au><au>CURIEL, David T</au><au>TERADA, Masaaki</au><au>ABE, Tatsuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient gene transduction by RGD-fiber modified recombinant adenovirus into dendritic cells</atitle><jtitle>Japanese journal of cancer research (Gann)</jtitle><addtitle>Jpn J Cancer Res</addtitle><date>2001-03-01</date><risdate>2001</risdate><volume>92</volume><issue>3</issue><spage>321</spage><epage>327</epage><pages>321-327</pages><issn>0910-5050</issn><eissn>1876-4673</eissn><coden>GANNA2</coden><abstract>Dendritic cells (DC) are important antigen-presenting cells in the development of an anti-tumor T cell response. 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| ispartof | Japanese journal of cancer research (Gann), 2001-03, Vol.92 (3), p.321-327 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Adenoviridae - genetics Amino Acid Sequence Antigens, CD - analysis Biological and medical sciences Biotechnology Cell Culture Techniques - methods Cells, Cultured Dendritic Cells - cytology Dendritic Cells - drug effects Dendritic Cells - immunology Flow Cytometry Fundamental and applied biological sciences. Psychology Gene therapy Genes, Reporter Genetic Vectors Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology Health. Pharmaceutical industry Humans Immunophenotyping Industrial applications and implications. Economical aspects Integrins - analysis Interleukin-4 - pharmacology Lipopolysaccharide Receptors - analysis Luciferases - analysis Luciferases - genetics Lung Neoplasms Oligopeptides Transfection - methods Tumor Cells, Cultured Tumor Necrosis Factor-alpha - pharmacology |
| title | Efficient gene transduction by RGD-fiber modified recombinant adenovirus into dendritic cells |
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