Determination of transferrin receptors on frozen sections of malignant B‐cell lymphomas by immunofluorescence with a monoclonal antibody
A series of 34 B‐cell lymphomas was studied for the presence of transferrin receptors by immunofluorescence on frozen tissue sections with a monoclonal antibody. These lymphomas were classified by light microscopic study and furthermore investigated by immunohistochemical and morphometric methods. T...
Gespeichert in:
| Veröffentlicht in: | Cancer 1986-09, Vol.58 (6), p.1252-1259 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1259 |
|---|---|
| container_issue | 6 |
| container_start_page | 1252 |
| container_title | Cancer |
| container_volume | 58 |
| creator | Oudemans, Pieter B. De La Rivière, Guy Brutel Hart, Guus A. M. van Heerde, Peter Scholte, Ger Vroom, Thea M. |
| description | A series of 34 B‐cell lymphomas was studied for the presence of transferrin receptors by immunofluorescence on frozen tissue sections with a monoclonal antibody. These lymphomas were classified by light microscopic study and furthermore investigated by immunohistochemical and morphometric methods. There were diffuse centrocytic, diffuse centroblastic, and follicular and diffuse centrocytic/centroblastic lymphomas. Significant differences in log mean nuclear area in these four histologic groups were found, as well as a significant correlation between log nuclear area and transferrin receptors as measured semiquantitatively by fluorescence intensity of the cell membranes. In addition, it was found that there is a strong correlation between the transferrin receptor activity and malignancy grading on histologic and morphometric basis. Therefore, the transferrin receptor determination on frozen sections appears to be a good method of malignancy grading. |
| doi_str_mv | 10.1002/1097-0142(19860915)58:6<1252::AID-CNCR2820580613>3.0.CO;2-M |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76989337</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76989337</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4983-8e106bf31d1959017f2c6ab99568c765a51ff093ede7c0a00ea8777b16887403</originalsourceid><addsrcrecordid>eNqVkcuKFDEUhgtRxnb0EYQsRHRRbS6dW48IY42XgRkbZBaCi5BKJ06kKmmTaoZy5dqVz-iTmKLbBl0IrkL4vxz-nK-qGgTnCEL8DEHJa4gW-AmSgkGJ6FMqluw5whQvl6fnZ3XzrnmPBYZUQIbICzKH82Z1guvLW9Xs8Pp2NYMQipouyIe71b2cP5crx5QcVUcEIk4JnVXfz-xgU--DHnwMIDowJB2ysyn5AJI1djPElEHJXIpfbQDZmgnNE9vrzn8KOgzg5c9vP4ztOtCN_eY69jqDdgS-77chum4bk83GBmPBjR-ugQZ9DNF0MegOlOe-jevxfnXH6S7bB_vzuLp6_eqqeVtfrN6cN6cXtVlIQWphEWStI2iNJJXlHw4bplspKROGM6opcg5KYteWG6ghtFpwzlvEhOALSI6rx7uxmxS_bG0eVO_zVF0HG7dZcSaFJIQX8OMONCnmnKxTm-R7nUaFoJpEqWnValq1-i1KUaGYmkQpVUSpP0UpoqBqVgqryzL94b7Gtu3t-jB7b6bkj_a5zkZ3rlgxPh8wLqjAnBXM7bAb39nx_xr-s-BfCfkF_eTBQQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76989337</pqid></control><display><type>article</type><title>Determination of transferrin receptors on frozen sections of malignant B‐cell lymphomas by immunofluorescence with a monoclonal antibody</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Oudemans, Pieter B. ; De La Rivière, Guy Brutel ; Hart, Guus A. M. ; van Heerde, Peter ; Scholte, Ger ; Vroom, Thea M.</creator><creatorcontrib>Oudemans, Pieter B. ; De La Rivière, Guy Brutel ; Hart, Guus A. M. ; van Heerde, Peter ; Scholte, Ger ; Vroom, Thea M.</creatorcontrib><description>A series of 34 B‐cell lymphomas was studied for the presence of transferrin receptors by immunofluorescence on frozen tissue sections with a monoclonal antibody. These lymphomas were classified by light microscopic study and furthermore investigated by immunohistochemical and morphometric methods. There were diffuse centrocytic, diffuse centroblastic, and follicular and diffuse centrocytic/centroblastic lymphomas. Significant differences in log mean nuclear area in these four histologic groups were found, as well as a significant correlation between log nuclear area and transferrin receptors as measured semiquantitatively by fluorescence intensity of the cell membranes. In addition, it was found that there is a strong correlation between the transferrin receptor activity and malignancy grading on histologic and morphometric basis. Therefore, the transferrin receptor determination on frozen sections appears to be a good method of malignancy grading.</description><identifier>ISSN: 0008-543X</identifier><identifier>EISSN: 1097-0142</identifier><identifier>DOI: 10.1002/1097-0142(19860915)58:6<1252::AID-CNCR2820580613>3.0.CO;2-M</identifier><identifier>PMID: 3017535</identifier><identifier>CODEN: CANCAR</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; Aged ; Antibodies, Monoclonal ; B-Lymphocytes - analysis ; B-Lymphocytes - pathology ; Biological and medical sciences ; Cell Membrane - analysis ; Female ; Fluorescent Antibody Technique ; Frozen Sections ; Hematologic and hematopoietic diseases ; Humans ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Lymphoma - analysis ; Lymphoma - pathology ; Male ; Medical sciences ; Middle Aged ; Receptors, Cell Surface - analysis ; Receptors, Transferrin</subject><ispartof>Cancer, 1986-09, Vol.58 (6), p.1252-1259</ispartof><rights>Copyright © 1986 American Cancer Society</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4983-8e106bf31d1959017f2c6ab99568c765a51ff093ede7c0a00ea8777b16887403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7858276$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3017535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oudemans, Pieter B.</creatorcontrib><creatorcontrib>De La Rivière, Guy Brutel</creatorcontrib><creatorcontrib>Hart, Guus A. M.</creatorcontrib><creatorcontrib>van Heerde, Peter</creatorcontrib><creatorcontrib>Scholte, Ger</creatorcontrib><creatorcontrib>Vroom, Thea M.</creatorcontrib><title>Determination of transferrin receptors on frozen sections of malignant B‐cell lymphomas by immunofluorescence with a monoclonal antibody</title><title>Cancer</title><addtitle>Cancer</addtitle><description>A series of 34 B‐cell lymphomas was studied for the presence of transferrin receptors by immunofluorescence on frozen tissue sections with a monoclonal antibody. These lymphomas were classified by light microscopic study and furthermore investigated by immunohistochemical and morphometric methods. There were diffuse centrocytic, diffuse centroblastic, and follicular and diffuse centrocytic/centroblastic lymphomas. Significant differences in log mean nuclear area in these four histologic groups were found, as well as a significant correlation between log nuclear area and transferrin receptors as measured semiquantitatively by fluorescence intensity of the cell membranes. In addition, it was found that there is a strong correlation between the transferrin receptor activity and malignancy grading on histologic and morphometric basis. Therefore, the transferrin receptor determination on frozen sections appears to be a good method of malignancy grading.</description><subject>Adult</subject><subject>Aged</subject><subject>Antibodies, Monoclonal</subject><subject>B-Lymphocytes - analysis</subject><subject>B-Lymphocytes - pathology</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane - analysis</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Frozen Sections</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Lymphoma - analysis</subject><subject>Lymphoma - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Receptors, Cell Surface - analysis</subject><subject>Receptors, Transferrin</subject><issn>0008-543X</issn><issn>1097-0142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkcuKFDEUhgtRxnb0EYQsRHRRbS6dW48IY42XgRkbZBaCi5BKJ06kKmmTaoZy5dqVz-iTmKLbBl0IrkL4vxz-nK-qGgTnCEL8DEHJa4gW-AmSgkGJ6FMqluw5whQvl6fnZ3XzrnmPBYZUQIbICzKH82Z1guvLW9Xs8Pp2NYMQipouyIe71b2cP5crx5QcVUcEIk4JnVXfz-xgU--DHnwMIDowJB2ysyn5AJI1djPElEHJXIpfbQDZmgnNE9vrzn8KOgzg5c9vP4ztOtCN_eY69jqDdgS-77chum4bk83GBmPBjR-ugQZ9DNF0MegOlOe-jevxfnXH6S7bB_vzuLp6_eqqeVtfrN6cN6cXtVlIQWphEWStI2iNJJXlHw4bplspKROGM6opcg5KYteWG6ghtFpwzlvEhOALSI6rx7uxmxS_bG0eVO_zVF0HG7dZcSaFJIQX8OMONCnmnKxTm-R7nUaFoJpEqWnValq1-i1KUaGYmkQpVUSpP0UpoqBqVgqryzL94b7Gtu3t-jB7b6bkj_a5zkZ3rlgxPh8wLqjAnBXM7bAb39nx_xr-s-BfCfkF_eTBQQ</recordid><startdate>19860915</startdate><enddate>19860915</enddate><creator>Oudemans, Pieter B.</creator><creator>De La Rivière, Guy Brutel</creator><creator>Hart, Guus A. M.</creator><creator>van Heerde, Peter</creator><creator>Scholte, Ger</creator><creator>Vroom, Thea M.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860915</creationdate><title>Determination of transferrin receptors on frozen sections of malignant B‐cell lymphomas by immunofluorescence with a monoclonal antibody</title><author>Oudemans, Pieter B. ; De La Rivière, Guy Brutel ; Hart, Guus A. M. ; van Heerde, Peter ; Scholte, Ger ; Vroom, Thea M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4983-8e106bf31d1959017f2c6ab99568c765a51ff093ede7c0a00ea8777b16887403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antibodies, Monoclonal</topic><topic>B-Lymphocytes - analysis</topic><topic>B-Lymphocytes - pathology</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane - analysis</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Frozen Sections</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Lymphoma - analysis</topic><topic>Lymphoma - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Receptors, Cell Surface - analysis</topic><topic>Receptors, Transferrin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oudemans, Pieter B.</creatorcontrib><creatorcontrib>De La Rivière, Guy Brutel</creatorcontrib><creatorcontrib>Hart, Guus A. M.</creatorcontrib><creatorcontrib>van Heerde, Peter</creatorcontrib><creatorcontrib>Scholte, Ger</creatorcontrib><creatorcontrib>Vroom, Thea M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oudemans, Pieter B.</au><au>De La Rivière, Guy Brutel</au><au>Hart, Guus A. M.</au><au>van Heerde, Peter</au><au>Scholte, Ger</au><au>Vroom, Thea M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of transferrin receptors on frozen sections of malignant B‐cell lymphomas by immunofluorescence with a monoclonal antibody</atitle><jtitle>Cancer</jtitle><addtitle>Cancer</addtitle><date>1986-09-15</date><risdate>1986</risdate><volume>58</volume><issue>6</issue><spage>1252</spage><epage>1259</epage><pages>1252-1259</pages><issn>0008-543X</issn><eissn>1097-0142</eissn><coden>CANCAR</coden><abstract>A series of 34 B‐cell lymphomas was studied for the presence of transferrin receptors by immunofluorescence on frozen tissue sections with a monoclonal antibody. These lymphomas were classified by light microscopic study and furthermore investigated by immunohistochemical and morphometric methods. There were diffuse centrocytic, diffuse centroblastic, and follicular and diffuse centrocytic/centroblastic lymphomas. Significant differences in log mean nuclear area in these four histologic groups were found, as well as a significant correlation between log nuclear area and transferrin receptors as measured semiquantitatively by fluorescence intensity of the cell membranes. In addition, it was found that there is a strong correlation between the transferrin receptor activity and malignancy grading on histologic and morphometric basis. Therefore, the transferrin receptor determination on frozen sections appears to be a good method of malignancy grading.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3017535</pmid><doi>10.1002/1097-0142(19860915)58:6<1252::AID-CNCR2820580613>3.0.CO;2-M</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-543X |
| ispartof | Cancer, 1986-09, Vol.58 (6), p.1252-1259 |
| issn | 0008-543X 1097-0142 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76989337 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult Aged Antibodies, Monoclonal B-Lymphocytes - analysis B-Lymphocytes - pathology Biological and medical sciences Cell Membrane - analysis Female Fluorescent Antibody Technique Frozen Sections Hematologic and hematopoietic diseases Humans Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Lymphoma - analysis Lymphoma - pathology Male Medical sciences Middle Aged Receptors, Cell Surface - analysis Receptors, Transferrin |
| title | Determination of transferrin receptors on frozen sections of malignant B‐cell lymphomas by immunofluorescence with a monoclonal antibody |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T19%3A48%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20transferrin%20receptors%20on%20frozen%20sections%20of%20malignant%20B%E2%80%90cell%20lymphomas%20by%20immunofluorescence%20with%20a%20monoclonal%20antibody&rft.jtitle=Cancer&rft.au=Oudemans,%20Pieter%20B.&rft.date=1986-09-15&rft.volume=58&rft.issue=6&rft.spage=1252&rft.epage=1259&rft.pages=1252-1259&rft.issn=0008-543X&rft.eissn=1097-0142&rft.coden=CANCAR&rft_id=info:doi/10.1002/1097-0142(19860915)58:6%3C1252::AID-CNCR2820580613%3E3.0.CO;2-M&rft_dat=%3Cproquest_cross%3E76989337%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76989337&rft_id=info:pmid/3017535&rfr_iscdi=true |