Expression of VP5 of infectious pancreatic necrosis virus strain VR299 is initiated at the second in-frame start codon
Infectious pancreatic necrosis virus (IPNV), a member of the Birnaviridae with two double-stranded RNA genome segments, encodes five proteins designated VP1 to VP5. To study the function of the 17 kDa nonstructural protein VP5 during virus replication several mutated IPNV genome segments A were cons...
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| Veröffentlicht in: | Journal of general virology 2001-04, Vol.82 (4), p.805-812 |
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| container_title | Journal of general virology |
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| creator | Weber, S Fichtner, D Mettenleiter, T.C Mundt, E |
| description | Infectious pancreatic necrosis virus (IPNV), a member of the Birnaviridae with two double-stranded RNA genome segments, encodes five proteins designated VP1 to VP5. To study the function of the 17 kDa nonstructural protein VP5 during virus replication several mutated IPNV genome segments A were constructed and included in a reverse genetics system for IPNV to obtain recombinant virus. Mutations between nt 68 and 85 or nt 94 and 103 in the noncoding region failed to yield viable virus. Only mutations located between nt 86 and 92 and downstream of nt 104 were tolerated, and viable virus could be generated. All IPNV generated showed no difference in replication compared with the wild-type IPNV, indicating that the absence of expression of VP5 did not influence virus growth in vitro. Furthermore, the results presented here indicate that initiation of translation of VP5 occurs at position 113, the second in-frame start codon. |
| doi_str_mv | 10.1099/0022-1317-82-4-805 |
| format | Article |
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To study the function of the 17 kDa nonstructural protein VP5 during virus replication several mutated IPNV genome segments A were constructed and included in a reverse genetics system for IPNV to obtain recombinant virus. Mutations between nt 68 and 85 or nt 94 and 103 in the noncoding region failed to yield viable virus. Only mutations located between nt 86 and 92 and downstream of nt 104 were tolerated, and viable virus could be generated. All IPNV generated showed no difference in replication compared with the wild-type IPNV, indicating that the absence of expression of VP5 did not influence virus growth in vitro. Furthermore, the results presented here indicate that initiation of translation of VP5 occurs at position 113, the second in-frame start codon.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-82-4-805</identifier><identifier>PMID: 11257185</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Birnaviridae ; Capsid - biosynthesis ; Capsid - genetics ; Capsid - physiology ; Capsid Proteins ; Cells, Cultured ; Codon ; double-stranded RNA ; genome ; Infectious pancreatic necrosis virus ; Infectious pancreatic necrosis virus - genetics ; microbial growth ; Molecular Sequence Data ; mutation ; start codon ; viral nonstructural proteins ; virus replication ; viruses ; VP1 protein ; VP2 protein ; VP3 protein ; VP4 protein ; VP5 protein</subject><ispartof>Journal of general virology, 2001-04, Vol.82 (4), p.805-812</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-7b5fef0997c6478b4cc6fdbd41f10dcd33e85b606e92f43dd85a6af8b449d80a3</citedby><cites>FETCH-LOGICAL-c430t-7b5fef0997c6478b4cc6fdbd41f10dcd33e85b606e92f43dd85a6af8b449d80a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11257185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weber, S</creatorcontrib><creatorcontrib>Fichtner, D</creatorcontrib><creatorcontrib>Mettenleiter, T.C</creatorcontrib><creatorcontrib>Mundt, E</creatorcontrib><title>Expression of VP5 of infectious pancreatic necrosis virus strain VR299 is initiated at the second in-frame start codon</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Infectious pancreatic necrosis virus (IPNV), a member of the Birnaviridae with two double-stranded RNA genome segments, encodes five proteins designated VP1 to VP5. To study the function of the 17 kDa nonstructural protein VP5 during virus replication several mutated IPNV genome segments A were constructed and included in a reverse genetics system for IPNV to obtain recombinant virus. Mutations between nt 68 and 85 or nt 94 and 103 in the noncoding region failed to yield viable virus. Only mutations located between nt 86 and 92 and downstream of nt 104 were tolerated, and viable virus could be generated. All IPNV generated showed no difference in replication compared with the wild-type IPNV, indicating that the absence of expression of VP5 did not influence virus growth in vitro. Furthermore, the results presented here indicate that initiation of translation of VP5 occurs at position 113, the second in-frame start codon.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Birnaviridae</subject><subject>Capsid - biosynthesis</subject><subject>Capsid - genetics</subject><subject>Capsid - physiology</subject><subject>Capsid Proteins</subject><subject>Cells, Cultured</subject><subject>Codon</subject><subject>double-stranded RNA</subject><subject>genome</subject><subject>Infectious pancreatic necrosis virus</subject><subject>Infectious pancreatic necrosis virus - genetics</subject><subject>microbial growth</subject><subject>Molecular Sequence Data</subject><subject>mutation</subject><subject>start codon</subject><subject>viral nonstructural proteins</subject><subject>virus replication</subject><subject>viruses</subject><subject>VP1 protein</subject><subject>VP2 protein</subject><subject>VP3 protein</subject><subject>VP4 protein</subject><subject>VP5 protein</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vVCEUhonR2Gn1D7hQVrqiApevuzRNtU2aaNR2S7h8zGDmwghMq_9ebmZi3bk64ZznvOS8LwCvCD4neBzfY0wpIgORSFHEkML8CVgRJjiiffwUrP4CJ-C01h8YE8a4fA5OCKFcEsVX4P7y1674WmNOMAd494UvJabgbYt5X-HOJFu8adHC5G3JNVZ4H0uf1FZMTPDuKx1H2LsxxRZN8w6aBtvGw-ptTq73UShm7u9mSoM2u5xegGfBbKt_eaxn4Pbj5feLK3Tz-dP1xYcbZNmAG5ITDz70Y6QVTKqJWSuCmxwjgWBn3TB4xSeBhR9pYINzihthQgfZ6BQ2wxl4e9Ddlfxz72vTc6zWb7cm-X6dlmJUgjHxX5BIRYdRsg7SA7h4UYsPelfibMpvTbBeYtGL63pxXSuqme6x9KXXR_X9NHv3uHLMoQPvDsAmrjcPsXi99mmO_Y8pZt39_kfqzYEMJmuzLrHq228UE4ExlrzLDX8AaHGgSA</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>Weber, S</creator><creator>Fichtner, D</creator><creator>Mettenleiter, T.C</creator><creator>Mundt, E</creator><general>Soc General Microbiol</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20010401</creationdate><title>Expression of VP5 of infectious pancreatic necrosis virus strain VR299 is initiated at the second in-frame start codon</title><author>Weber, S ; Fichtner, D ; Mettenleiter, T.C ; Mundt, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-7b5fef0997c6478b4cc6fdbd41f10dcd33e85b606e92f43dd85a6af8b449d80a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Birnaviridae</topic><topic>Capsid - biosynthesis</topic><topic>Capsid - genetics</topic><topic>Capsid - physiology</topic><topic>Capsid Proteins</topic><topic>Cells, Cultured</topic><topic>Codon</topic><topic>double-stranded RNA</topic><topic>genome</topic><topic>Infectious pancreatic necrosis virus</topic><topic>Infectious pancreatic necrosis virus - genetics</topic><topic>microbial growth</topic><topic>Molecular Sequence Data</topic><topic>mutation</topic><topic>start codon</topic><topic>viral nonstructural proteins</topic><topic>virus replication</topic><topic>viruses</topic><topic>VP1 protein</topic><topic>VP2 protein</topic><topic>VP3 protein</topic><topic>VP4 protein</topic><topic>VP5 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weber, S</creatorcontrib><creatorcontrib>Fichtner, D</creatorcontrib><creatorcontrib>Mettenleiter, T.C</creatorcontrib><creatorcontrib>Mundt, E</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weber, S</au><au>Fichtner, D</au><au>Mettenleiter, T.C</au><au>Mundt, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of VP5 of infectious pancreatic necrosis virus strain VR299 is initiated at the second in-frame start codon</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2001-04-01</date><risdate>2001</risdate><volume>82</volume><issue>4</issue><spage>805</spage><epage>812</epage><pages>805-812</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Infectious pancreatic necrosis virus (IPNV), a member of the Birnaviridae with two double-stranded RNA genome segments, encodes five proteins designated VP1 to VP5. To study the function of the 17 kDa nonstructural protein VP5 during virus replication several mutated IPNV genome segments A were constructed and included in a reverse genetics system for IPNV to obtain recombinant virus. Mutations between nt 68 and 85 or nt 94 and 103 in the noncoding region failed to yield viable virus. Only mutations located between nt 86 and 92 and downstream of nt 104 were tolerated, and viable virus could be generated. All IPNV generated showed no difference in replication compared with the wild-type IPNV, indicating that the absence of expression of VP5 did not influence virus growth in vitro. Furthermore, the results presented here indicate that initiation of translation of VP5 occurs at position 113, the second in-frame start codon.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>11257185</pmid><doi>10.1099/0022-1317-82-4-805</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 2001-04, Vol.82 (4), p.805-812 |
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| language | eng |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Base Sequence Birnaviridae Capsid - biosynthesis Capsid - genetics Capsid - physiology Capsid Proteins Cells, Cultured Codon double-stranded RNA genome Infectious pancreatic necrosis virus Infectious pancreatic necrosis virus - genetics microbial growth Molecular Sequence Data mutation start codon viral nonstructural proteins virus replication viruses VP1 protein VP2 protein VP3 protein VP4 protein VP5 protein |
| title | Expression of VP5 of infectious pancreatic necrosis virus strain VR299 is initiated at the second in-frame start codon |
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