Purified and Refolded Recombinant Bonnet Monkey (Macaca radiata) Zona Pellucida Glycoprotein-B Expressed in Escherichia coli Binds to Spermatozoa

Bonnet monkey ( Macaca radiata ) zona pellucida glycoprotein-B (bmZPB), excluding the N -terminal signal sequence and the C -terminus transmembrane-like domain, has been expressed in Escherichia coli as polyhistidine fusion protein. A requirement of 4 M urea to maintain the purified protein in solub...

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Veröffentlicht in:	Biology of reproduction 2001-04, Vol.64 (4), p.1147-1152
Hauptverfasser:	GOVIND, Chhabi K, GAHLAY, Gagandeep K, CHOUDHURY, Sangeeta, GUPTA, Satish K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Blotting, Western Circular Dichroism Egg Proteins - chemistry Egg Proteins - genetics Egg Proteins - metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Female Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology Gene Expression Histidine Macaca radiata Male Mammalian male genital system Membrane Glycoproteins - chemistry Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Morphology. Physiology Peptides - genetics Protein Denaturation Protein Folding Receptors, Cell Surface Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Spermatozoa - metabolism Vertebrates: reproduction Zona Pellucida Glycoproteins
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