Detection of avocado sunblotch viroid variants using fluorescent single-strand conformation polymorphism analysis

A specific reverse transcription‐polymerase chain reaction (RT‐PCR) protocol has been developed for routine detection of avocado sunblotch viroid (ASBVd). Modifications in this diagnostic technique were made to enable fluorescent detection and variant identification using automated capillary electro...

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Veröffentlicht in:	Electrophoresis 2001-02, Vol.22 (3), p.427-432
Hauptverfasser:	Schnell, Raymond J., Olano, Cecile T., Kuhn, David N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Capillary electrophoresis DNA, Viral Electrophoresis, Capillary - methods Fluorescent Dyes Genetic Variation Lauraceae - virology Molecular Sequence Data Plant Viruses - genetics Polymorphism, Single-Stranded Conformational Reverse Transcriptase Polymerase Chain Reaction RNA Viruses - genetics RNA, Viral - analysis Sequence variants Single-strand conformation polymorphism analysis Viroids Viroids - genetics
Online-Zugang:	Volltext
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