Gene mapping and physical arrangements of human chromatin in transformed, hybrid cells: fluorescent and autoradiographic in situ hybridization compared
We compare a fluorescent in situ hybridization technique, using N-acetoxy-2-acetylaminofluorene (N-ACO-AAF) modified DNA adducts, with 3H-labeled DNA in situ hybridization for visualizing human transgenomes in HRAS1-selected, chromosome-mediated gene transfer (CMGT), and mapping chromosomal SV40 in...
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| Veröffentlicht in: | Somatic cell and molecular genetics 1986-07, Vol.12 (4), p.313-324 |
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| creator | MITCHELL, A. R AMBROS, P GOSDEN, J. R MORTEN, J. E. N PORTEOUS, D. J |
| description | We compare a fluorescent in situ hybridization technique, using N-acetoxy-2-acetylaminofluorene (N-ACO-AAF) modified DNA adducts, with 3H-labeled DNA in situ hybridization for visualizing human transgenomes in HRAS1-selected, chromosome-mediated gene transfer (CMGT), and mapping chromosomal SV40 in an SV40-transformed, human-mouse hybrid cell line. We demonstrate that individual HRAS1-CMGTs may contain multiple fragments of human chromatin. We deduce that the CMGT process can involve interstitial loss of mouse chromatin. We conclude that the N-ACO-AAF technique gives finer resolution than 3H-labeled in situ hybridization. However, 3H-labeling is more sensitive and has allowed us to sublocalize SV40 in C121 to the region 7q31-35. |
| doi_str_mv | 10.1007/BF01570725 |
| format | Article |
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R ; AMBROS, P ; GOSDEN, J. R ; MORTEN, J. E. N ; PORTEOUS, D. J</creator><creatorcontrib>MITCHELL, A. R ; AMBROS, P ; GOSDEN, J. R ; MORTEN, J. E. N ; PORTEOUS, D. J</creatorcontrib><description>We compare a fluorescent in situ hybridization technique, using N-acetoxy-2-acetylaminofluorene (N-ACO-AAF) modified DNA adducts, with 3H-labeled DNA in situ hybridization for visualizing human transgenomes in HRAS1-selected, chromosome-mediated gene transfer (CMGT), and mapping chromosomal SV40 in an SV40-transformed, human-mouse hybrid cell line. We demonstrate that individual HRAS1-CMGTs may contain multiple fragments of human chromatin. We deduce that the CMGT process can involve interstitial loss of mouse chromatin. We conclude that the N-ACO-AAF technique gives finer resolution than 3H-labeled in situ hybridization. However, 3H-labeling is more sensitive and has allowed us to sublocalize SV40 in C121 to the region 7q31-35.</description><identifier>ISSN: 0740-7750</identifier><identifier>EISSN: 1572-9931</identifier><identifier>DOI: 10.1007/BF01570725</identifier><identifier>PMID: 3016913</identifier><identifier>CODEN: SCMGDN</identifier><language>eng</language><publisher>London: Plenum</publisher><subject>Animal cells ; Animals ; Biological and medical sciences ; Biotechnology ; Cell Transformation, Viral ; Chromatin - analysis ; Chromatin - genetics ; Chromosome Mapping ; Chromosomes, Human, 6-12 and X ; Cytogenetics ; DNA - genetics ; DNA Restriction Enzymes ; Eukaryotic cell cultures ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; Genetical aspects ; Genetics of eukaryotes. Biological and molecular evolution ; Humans ; Hybrid Cells ; Methods and miscellaneous ; Methods. Procedures. Technologies ; Mice ; Nucleic Acid Hybridization ; Simian virus 40 - genetics</subject><ispartof>Somatic cell and molecular genetics, 1986-07, Vol.12 (4), p.313-324</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c257t-356c4221e07a682182b2db4de2ed8525de367c51223fe04399dc4865586834cb3</citedby><cites>FETCH-LOGICAL-c257t-356c4221e07a682182b2db4de2ed8525de367c51223fe04399dc4865586834cb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8088311$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3016913$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MITCHELL, A. R</creatorcontrib><creatorcontrib>AMBROS, P</creatorcontrib><creatorcontrib>GOSDEN, J. R</creatorcontrib><creatorcontrib>MORTEN, J. E. N</creatorcontrib><creatorcontrib>PORTEOUS, D. J</creatorcontrib><title>Gene mapping and physical arrangements of human chromatin in transformed, hybrid cells: fluorescent and autoradiographic in situ hybridization compared</title><title>Somatic cell and molecular genetics</title><addtitle>Somat Cell Mol Genet</addtitle><description>We compare a fluorescent in situ hybridization technique, using N-acetoxy-2-acetylaminofluorene (N-ACO-AAF) modified DNA adducts, with 3H-labeled DNA in situ hybridization for visualizing human transgenomes in HRAS1-selected, chromosome-mediated gene transfer (CMGT), and mapping chromosomal SV40 in an SV40-transformed, human-mouse hybrid cell line. We demonstrate that individual HRAS1-CMGTs may contain multiple fragments of human chromatin. We deduce that the CMGT process can involve interstitial loss of mouse chromatin. We conclude that the N-ACO-AAF technique gives finer resolution than 3H-labeled in situ hybridization. However, 3H-labeling is more sensitive and has allowed us to sublocalize SV40 in C121 to the region 7q31-35.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Transformation, Viral</subject><subject>Chromatin - analysis</subject><subject>Chromatin - genetics</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, 6-12 and X</subject><subject>Cytogenetics</subject><subject>DNA - genetics</subject><subject>DNA Restriction Enzymes</subject><subject>Eukaryotic cell cultures</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetical aspects</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Humans</subject><subject>Hybrid Cells</subject><subject>Methods and miscellaneous</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Nucleic Acid Hybridization</subject><subject>Simian virus 40 - genetics</subject><issn>0740-7750</issn><issn>1572-9931</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1r3TAUhkVJSG7TLt0LGkKHUqf6tORubWiSQiBLOxtZOr5WsS1HsoebP5K_W7kxyRgQSHCe9wGdF6EPlFxQQtTXH1eESkUUk2_QLr9YUVWcHqEdUYIUSklyit6m9JcQojWXJ-iEE1pWlO_Q4zWMgAczTX7cYzM6PHWH5K3psYnRjHsYYJwTDi3ulsGM2HYxDGb2I85nzkRqQxzAfcHdoYneYQt9n77htl9ChGRz-r_WLHOIxvmwj2bqvF3jyc_LFvMP2RmyPgyTieDeoePW9Aneb_cZ-nP18_flTXF7d_3r8vttYZlUc8FlaQVjFIgypWZUs4a5Rjhg4LRk0gEvlZWUMd4CEbyqnBW6lFKXmgvb8DP06ck7xXC_QJrrwaf1C2aEsKRalZVSRNBXQSpEVSq2gp-fQBtDShHaeop-MPFQU1KvddUvdWX442ZdmrzEZ3TrJ8_Pt7lJuZM279v69IzptU9K-T9zHZ5k</recordid><startdate>198607</startdate><enddate>198607</enddate><creator>MITCHELL, A. R</creator><creator>AMBROS, P</creator><creator>GOSDEN, J. R</creator><creator>MORTEN, J. E. 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Biological and molecular evolution</topic><topic>Humans</topic><topic>Hybrid Cells</topic><topic>Methods and miscellaneous</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Nucleic Acid Hybridization</topic><topic>Simian virus 40 - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MITCHELL, A. R</creatorcontrib><creatorcontrib>AMBROS, P</creatorcontrib><creatorcontrib>GOSDEN, J. R</creatorcontrib><creatorcontrib>MORTEN, J. E. N</creatorcontrib><creatorcontrib>PORTEOUS, D. 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We deduce that the CMGT process can involve interstitial loss of mouse chromatin. We conclude that the N-ACO-AAF technique gives finer resolution than 3H-labeled in situ hybridization. However, 3H-labeling is more sensitive and has allowed us to sublocalize SV40 in C121 to the region 7q31-35.</abstract><cop>London</cop><cop>New York, NY</cop><pub>Plenum</pub><pmid>3016913</pmid><doi>10.1007/BF01570725</doi><tpages>12</tpages></addata></record> |
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| ispartof | Somatic cell and molecular genetics, 1986-07, Vol.12 (4), p.313-324 |
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| language | eng |
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| subjects | Animal cells Animals Biological and medical sciences Biotechnology Cell Transformation, Viral Chromatin - analysis Chromatin - genetics Chromosome Mapping Chromosomes, Human, 6-12 and X Cytogenetics DNA - genetics DNA Restriction Enzymes Eukaryotic cell cultures Fluorescent Dyes Fundamental and applied biological sciences. Psychology Genetical aspects Genetics of eukaryotes. Biological and molecular evolution Humans Hybrid Cells Methods and miscellaneous Methods. Procedures. Technologies Mice Nucleic Acid Hybridization Simian virus 40 - genetics |
| title | Gene mapping and physical arrangements of human chromatin in transformed, hybrid cells: fluorescent and autoradiographic in situ hybridization compared |
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