Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes

Transgenic rhesus monkeys carrying the green fluorescent protein (GFP) gene were produced by injecting pseudotyped replication-defective retroviral vector into the perivitelline space of 224 mature rhesus oocytes, later fertilized by intracytoplasmic sperm injection. Of the three males born from 20...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2001-01, Vol.291 (5502), p.309-312
Hauptverfasser:	Chan, A. W. S., Chong, K. Y., Martinovich, C., Simerly, C., Schatten, G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal genetic engineering Animals Animals, Genetically Modified Animals, Newborn Biological and medical sciences Biotechnology Blotting, Southern DNA Embryo Transfer Embryonic and Fetal Development Female Fetus Fluorescence Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Expression Gene therapy Gene Transfer Techniques Gene Transfer, Horizontal Genes Genetic engineering Genetic Vectors Genetically modified animals Green Fluorescent Proteins Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Intracytoplasmic sperm injections Luminescent Proteins - genetics Macaca mulatta - genetics Male Males Methods Moloney murine leukemia virus - genetics Monkeys Monkeys & apes Neonates Oocytes Polymerase Chain Reaction Pregnancy Pregnancy Outcome Proteins Reverse transcriptase polymerase chain reaction Sperm Injections, Intracytoplasmic Transgenes Transgenic animals Twins Young Children
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