Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes
Transgenic rhesus monkeys carrying the green fluorescent protein (GFP) gene were produced by injecting pseudotyped replication-defective retroviral vector into the perivitelline space of 224 mature rhesus oocytes, later fertilized by intracytoplasmic sperm injection. Of the three males born from 20...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2001-01, Vol.291 (5502), p.309-312 |
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| creator | Chan, A. W. S. Chong, K. Y. Martinovich, C. Simerly, C. Schatten, G. |
| description | Transgenic rhesus monkeys carrying the green fluorescent protein (GFP) gene were produced by injecting pseudotyped replication-defective retroviral vector into the perivitelline space of 224 mature rhesus oocytes, later fertilized by intracytoplasmic sperm injection. Of the three males born from 20 embryo transfers, one was transgenic when accessible tissues were assayed for transgene DNA and messenger RNA. All tissues that were studied from a fraternal set of twins, miscarried at 73 days, carried the transgene, as confirmed by Southern analyses, and the GFP transgene reporter was detected by both direct and indirect fluorescence imaging. |
| doi_str_mv | 10.1126/science.291.5502.309 |
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W. S. ; Chong, K. Y. ; Martinovich, C. ; Simerly, C. ; Schatten, G.</creator><creatorcontrib>Chan, A. W. S. ; Chong, K. Y. ; Martinovich, C. ; Simerly, C. ; Schatten, G.</creatorcontrib><description>Transgenic rhesus monkeys carrying the green fluorescent protein (GFP) gene were produced by injecting pseudotyped replication-defective retroviral vector into the perivitelline space of 224 mature rhesus oocytes, later fertilized by intracytoplasmic sperm injection. Of the three males born from 20 embryo transfers, one was transgenic when accessible tissues were assayed for transgene DNA and messenger RNA. All tissues that were studied from a fraternal set of twins, miscarried at 73 days, carried the transgene, as confirmed by Southern analyses, and the GFP transgene reporter was detected by both direct and indirect fluorescence imaging.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.291.5502.309</identifier><identifier>PMID: 11209082</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Animal genetic engineering ; Animals ; Animals, Genetically Modified ; Animals, Newborn ; Biological and medical sciences ; Biotechnology ; Blotting, Southern ; DNA ; Embryo Transfer ; Embryonic and Fetal Development ; Female ; Fetus ; Fluorescence ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene therapy ; Gene Transfer Techniques ; Gene Transfer, Horizontal ; Genes ; Genetic engineering ; Genetic Vectors ; Genetically modified animals ; Green Fluorescent Proteins ; Health. Pharmaceutical industry ; Industrial applications and implications. Economical aspects ; Intracytoplasmic sperm injections ; Luminescent Proteins - genetics ; Macaca mulatta - genetics ; Male ; Males ; Methods ; Moloney murine leukemia virus - genetics ; Monkeys ; Monkeys & apes ; Neonates ; Oocytes ; Polymerase Chain Reaction ; Pregnancy ; Pregnancy Outcome ; Proteins ; Reverse transcriptase polymerase chain reaction ; Sperm Injections, Intracytoplasmic ; Transgenes ; Transgenic animals ; Twins ; Young Children</subject><ispartof>Science (American Association for the Advancement of Science), 2001-01, Vol.291 (5502), p.309-312</ispartof><rights>Copyright 2001 American Association for the Advancement of Science</rights><rights>2001 INIST-CNRS</rights><rights>COPYRIGHT 2001 American Association for the Advancement of Science</rights><rights>COPYRIGHT 2001 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Jan 12, 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c716t-4ac49e1b6121a391fe314cdbc8231707760c9c37cbc2bda0c880c8c4900fcb753</citedby><cites>FETCH-LOGICAL-c716t-4ac49e1b6121a391fe314cdbc8231707760c9c37cbc2bda0c880c8c4900fcb753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3082348$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3082348$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=885365$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11209082$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chan, A. W. S.</creatorcontrib><creatorcontrib>Chong, K. Y.</creatorcontrib><creatorcontrib>Martinovich, C.</creatorcontrib><creatorcontrib>Simerly, C.</creatorcontrib><creatorcontrib>Schatten, G.</creatorcontrib><title>Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Transgenic rhesus monkeys carrying the green fluorescent protein (GFP) gene were produced by injecting pseudotyped replication-defective retroviral vector into the perivitelline space of 224 mature rhesus oocytes, later fertilized by intracytoplasmic sperm injection. Of the three males born from 20 embryo transfers, one was transgenic when accessible tissues were assayed for transgene DNA and messenger RNA. All tissues that were studied from a fraternal set of twins, miscarried at 73 days, carried the transgene, as confirmed by Southern analyses, and the GFP transgene reporter was detected by both direct and indirect fluorescence imaging.</description><subject>Animal genetic engineering</subject><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Southern</subject><subject>DNA</subject><subject>Embryo Transfer</subject><subject>Embryonic and Fetal Development</subject><subject>Female</subject><subject>Fetus</subject><subject>Fluorescence</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene therapy</subject><subject>Gene Transfer Techniques</subject><subject>Gene Transfer, Horizontal</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetic Vectors</subject><subject>Genetically modified animals</subject><subject>Green Fluorescent Proteins</subject><subject>Health. Pharmaceutical industry</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Intracytoplasmic sperm injections</subject><subject>Luminescent Proteins - genetics</subject><subject>Macaca mulatta - genetics</subject><subject>Male</subject><subject>Males</subject><subject>Methods</subject><subject>Moloney murine leukemia virus - genetics</subject><subject>Monkeys</subject><subject>Monkeys & apes</subject><subject>Neonates</subject><subject>Oocytes</subject><subject>Polymerase Chain Reaction</subject><subject>Pregnancy</subject><subject>Pregnancy Outcome</subject><subject>Proteins</subject><subject>Reverse transcriptase polymerase chain reaction</subject><subject>Sperm Injections, Intracytoplasmic</subject><subject>Transgenes</subject><subject>Transgenic animals</subject><subject>Twins</subject><subject>Young Children</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN0l1v0zAUBmALgVgp_IMJRUwCLpbijySOL0cFZaKjCAa3lnNyUqWk8bCTaf33OLQaKqqgiqxI8XOOY_sl5JTRCWM8e-OhxhZwwhWbpCnlE0HVAzJiVKWx4lQ8JCNKRRbnVKYn5In3K0rDnBKPyUloQBXN-Yh8vHam9Utsa4iubPsDNz767GzZA5ZRsYm-YOfsbe1ME82wxeg3r9BFddvZ6Mp0vcNoYWHToX9KHlWm8fhs9x6Tb-_fXU8_xPPF7HJ6MY9BsqyLEwOJQlZkjDMjFKtQsATKAnIumKRSZhQUCAkF8KI0FPI8jFBDaQWFTMWYvNz2vXH2Z4--0-vaAzaNadH2XstMyWTY6Zi8-jdMRCoyyf7fksmwbi5EgC_-givbuzZsV3MmUsV5wgI636KlaVDXbWU7ZyAcMoZztC1Wdfh8kSmVsYQNvxkf4OEpcV3DIf96zwfS4V23NL33-vLrp6Pp4vvR9O3sWJrP5nv0_BAF2zS4RB1yMV3s8WTLwVnvHVb6xtVr4zaaUT3EXu9ir0Ps9RB7HWIfyp7vbqUv1lj-KdrlPICzHTAeTFOFFEPt712ehxwMKTjdqpXvrLufFUOHJBe_AM7PD3E</recordid><startdate>20010112</startdate><enddate>20010112</enddate><creator>Chan, A. 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Psychology</topic><topic>Gene Expression</topic><topic>Gene therapy</topic><topic>Gene Transfer Techniques</topic><topic>Gene Transfer, Horizontal</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genetic Vectors</topic><topic>Genetically modified animals</topic><topic>Green Fluorescent Proteins</topic><topic>Health. Pharmaceutical industry</topic><topic>Industrial applications and implications. 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| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2001-01, Vol.291 (5502), p.309-312 |
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| source | Jstor Complete Legacy; MEDLINE; Science Online_科学在线 |
| subjects | Animal genetic engineering Animals Animals, Genetically Modified Animals, Newborn Biological and medical sciences Biotechnology Blotting, Southern DNA Embryo Transfer Embryonic and Fetal Development Female Fetus Fluorescence Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Expression Gene therapy Gene Transfer Techniques Gene Transfer, Horizontal Genes Genetic engineering Genetic Vectors Genetically modified animals Green Fluorescent Proteins Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Intracytoplasmic sperm injections Luminescent Proteins - genetics Macaca mulatta - genetics Male Males Methods Moloney murine leukemia virus - genetics Monkeys Monkeys & apes Neonates Oocytes Polymerase Chain Reaction Pregnancy Pregnancy Outcome Proteins Reverse transcriptase polymerase chain reaction Sperm Injections, Intracytoplasmic Transgenes Transgenic animals Twins Young Children |
| title | Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes |
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