Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N-terminal amino acid sequence analysis
Aspergillus species are common airborne fungi that have been identified as causative agents of extrinsic bronchial asthma. More than 10 allergens from A. fumigatus have been recently characterized by cDNA cloning. The objective of this study is to identify A. fumigatus allergens through immunoblot a...
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| Veröffentlicht in: | Clinical and experimental allergy 2001-02, Vol.31 (2), p.295-302 |
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| creator | Shen, H.-D. Lin, W.-L. Tam, M. F. Chou, H. Wang, C.-W. Tsai, J.-J. Wang, S.-R. Han, S.-H. |
| description | Aspergillus species are common airborne fungi that have been identified as causative agents of extrinsic bronchial asthma. More than 10 allergens from A. fumigatus have been recently characterized by cDNA cloning.
The objective of this study is to identify A. fumigatus allergens through immunoblot analysis using sera from asthmatic patients.
IgE‐binding components of A. fumigatus and IgE cross‐reactivity among allergens of different prevalent airborne fungal species were analysed by immunoblot and immunoblot inhibition, respectively, using sera from asthmatic patients. The N‐terminal amino acid sequences of major allergens identified were determined by Edman degradation.
Among two batches (70 and 41 sera) of asthmatic sera tested, 19 (27%) and 14 (34%), respectively, have IgE immunoblot reactivity towards components of A. fumigatus. A 34‐kDa protein that reacts with IgE antibodies in 15 (79%) and 11 (79%) of the 19 and 14 positive samples, respectively, may be considered a major allergen of A. fumigatus. The N‐terminal amino acid sequences of the 34 kDa major allergen and the 30.5 and 30 kDa IgE‐binding components of A. fumigatus showed sequence identity to that of the vacuolar serine proteinase from A. fumigatus. The results from immunoblot inhibition show IgE cross‐reactivity among major allergens of A. fumigatus, P. notatum and P. oxalicum.
Results obtained suggest that the 34 kDa major allergen of A. fumigatus may be a vacuolar serine proteinase. There is IgE cross‐reactivity among serine proteinase allergens of A. fumigatus, P. notatum and P. oxalicum. |
| doi_str_mv | 10.1046/j.1365-2222.2001.01026.x |
| format | Article |
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The objective of this study is to identify A. fumigatus allergens through immunoblot analysis using sera from asthmatic patients.
IgE‐binding components of A. fumigatus and IgE cross‐reactivity among allergens of different prevalent airborne fungal species were analysed by immunoblot and immunoblot inhibition, respectively, using sera from asthmatic patients. The N‐terminal amino acid sequences of major allergens identified were determined by Edman degradation.
Among two batches (70 and 41 sera) of asthmatic sera tested, 19 (27%) and 14 (34%), respectively, have IgE immunoblot reactivity towards components of A. fumigatus. A 34‐kDa protein that reacts with IgE antibodies in 15 (79%) and 11 (79%) of the 19 and 14 positive samples, respectively, may be considered a major allergen of A. fumigatus. The N‐terminal amino acid sequences of the 34 kDa major allergen and the 30.5 and 30 kDa IgE‐binding components of A. fumigatus showed sequence identity to that of the vacuolar serine proteinase from A. fumigatus. The results from immunoblot inhibition show IgE cross‐reactivity among major allergens of A. fumigatus, P. notatum and P. oxalicum.
Results obtained suggest that the 34 kDa major allergen of A. fumigatus may be a vacuolar serine proteinase. There is IgE cross‐reactivity among serine proteinase allergens of A. fumigatus, P. notatum and P. oxalicum.</description><identifier>ISSN: 0954-7894</identifier><identifier>EISSN: 1365-2222</identifier><identifier>DOI: 10.1046/j.1365-2222.2001.01026.x</identifier><identifier>PMID: 11251631</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science, Ltd</publisher><subject>A. fumigatus ; Adolescent ; Adult ; Aged ; allergens ; Allergens - chemistry ; Allergens - immunology ; Allergic diseases ; Antigens, Fungal - chemistry ; Antigens, Fungal - immunology ; Aspergillus fumigatus ; Aspergillus fumigatus - enzymology ; Aspergillus fumigatus - immunology ; Asthma - immunology ; Biological and medical sciences ; Cross Reactions ; Electrophoresis, Gel, Two-Dimensional ; Humans ; immunoblot ; Immunoblotting ; Immunoglobulin E - immunology ; Immunopathology ; Medical sciences ; Middle Aged ; N-terminal amino acid sequence ; Penicillium - immunology ; Respiratory and ent allergic diseases ; Sequence Analysis, Protein ; Sequence Homology, Amino Acid ; Serine Endopeptidases - chemistry ; Serine Endopeptidases - immunology ; Vacuoles - enzymology</subject><ispartof>Clinical and experimental allergy, 2001-02, Vol.31 (2), p.295-302</ispartof><rights>2001 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Feb 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5556-438ebd626a1fb9ee05a0c6bf4ff051216cbc61e9233a2eb93a0fec87b07389a63</citedby><cites>FETCH-LOGICAL-c5556-438ebd626a1fb9ee05a0c6bf4ff051216cbc61e9233a2eb93a0fec87b07389a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2222.2001.01026.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2222.2001.01026.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=970608$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11251631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, H.-D.</creatorcontrib><creatorcontrib>Lin, W.-L.</creatorcontrib><creatorcontrib>Tam, M. F.</creatorcontrib><creatorcontrib>Chou, H.</creatorcontrib><creatorcontrib>Wang, C.-W.</creatorcontrib><creatorcontrib>Tsai, J.-J.</creatorcontrib><creatorcontrib>Wang, S.-R.</creatorcontrib><creatorcontrib>Han, S.-H.</creatorcontrib><title>Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N-terminal amino acid sequence analysis</title><title>Clinical and experimental allergy</title><addtitle>Clin Exp Allergy</addtitle><description>Aspergillus species are common airborne fungi that have been identified as causative agents of extrinsic bronchial asthma. More than 10 allergens from A. fumigatus have been recently characterized by cDNA cloning.
The objective of this study is to identify A. fumigatus allergens through immunoblot analysis using sera from asthmatic patients.
IgE‐binding components of A. fumigatus and IgE cross‐reactivity among allergens of different prevalent airborne fungal species were analysed by immunoblot and immunoblot inhibition, respectively, using sera from asthmatic patients. The N‐terminal amino acid sequences of major allergens identified were determined by Edman degradation.
Among two batches (70 and 41 sera) of asthmatic sera tested, 19 (27%) and 14 (34%), respectively, have IgE immunoblot reactivity towards components of A. fumigatus. A 34‐kDa protein that reacts with IgE antibodies in 15 (79%) and 11 (79%) of the 19 and 14 positive samples, respectively, may be considered a major allergen of A. fumigatus. The N‐terminal amino acid sequences of the 34 kDa major allergen and the 30.5 and 30 kDa IgE‐binding components of A. fumigatus showed sequence identity to that of the vacuolar serine proteinase from A. fumigatus. The results from immunoblot inhibition show IgE cross‐reactivity among major allergens of A. fumigatus, P. notatum and P. oxalicum.
Results obtained suggest that the 34 kDa major allergen of A. fumigatus may be a vacuolar serine proteinase. There is IgE cross‐reactivity among serine proteinase allergens of A. fumigatus, P. notatum and P. oxalicum.</description><subject>A. fumigatus</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>allergens</subject><subject>Allergens - chemistry</subject><subject>Allergens - immunology</subject><subject>Allergic diseases</subject><subject>Antigens, Fungal - chemistry</subject><subject>Antigens, Fungal - immunology</subject><subject>Aspergillus fumigatus</subject><subject>Aspergillus fumigatus - enzymology</subject><subject>Aspergillus fumigatus - immunology</subject><subject>Asthma - immunology</subject><subject>Biological and medical sciences</subject><subject>Cross Reactions</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Humans</subject><subject>immunoblot</subject><subject>Immunoblotting</subject><subject>Immunoglobulin E - immunology</subject><subject>Immunopathology</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>N-terminal amino acid sequence</subject><subject>Penicillium - immunology</subject><subject>Respiratory and ent allergic diseases</subject><subject>Sequence Analysis, Protein</subject><subject>Sequence Homology, Amino Acid</subject><subject>Serine Endopeptidases - chemistry</subject><subject>Serine Endopeptidases - immunology</subject><subject>Vacuoles - enzymology</subject><issn>0954-7894</issn><issn>1365-2222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcGO0zAQhiMEYsvCKyALJG4JdlI78YFDVZayUrUcWMTRmrjjysWJi51A-yS8Ls62KhIXsCzNSPP9Mx7_WUYYLRidi7e7glWC52U6RUkpKyijpSgOj7LZpfA4m1HJ53ndyPlV9izGHaW04rJ5ml0xVnImKjbLft1usB-ssRoG63viDfkBevQOAokYbI9kH_yAtoeIBCIB0sHOBwLOYdjig2IR9ym3zo2RmLGzWxhS1h6J7bqx963zw2D7LYF-Q-7yAUOX2jkCKXgC2m7SqO8j9jpNSIVjtPF59sSAi_jiHK-zLx9u7pcf8_Wn1e1ysc4151zk86rBdiNKAcy0EpFyoFq0Zm4M5axkQrdaMJRlVUGJrayAGtRN3dK6aiSI6jp7c-qbtkxPiIPqbNToHPTox6hqIesy3X-CLP0zr5lM4Ku_wJ0fQ1orMVJKWjdsGtucIB18jAGN2gfbQTgqRtVksdqpyUk1Oakmi9WDxeqQpC_P_ce2w80f4dnTBLw-AxA1OBOg1zZeOFlTQZtEvTtRP63D43-PV8ubxZQlfX7S2zjg4aKH8E2Juqq5-nq3Uqu1vC_ff2ap2W8af9Lw</recordid><startdate>200102</startdate><enddate>200102</enddate><creator>Shen, H.-D.</creator><creator>Lin, W.-L.</creator><creator>Tam, M. F.</creator><creator>Chou, H.</creator><creator>Wang, C.-W.</creator><creator>Tsai, J.-J.</creator><creator>Wang, S.-R.</creator><creator>Han, S.-H.</creator><general>Blackwell Science, Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>200102</creationdate><title>Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N-terminal amino acid sequence analysis</title><author>Shen, H.-D. ; Lin, W.-L. ; Tam, M. F. ; Chou, H. ; Wang, C.-W. ; Tsai, J.-J. ; Wang, S.-R. ; Han, S.-H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5556-438ebd626a1fb9ee05a0c6bf4ff051216cbc61e9233a2eb93a0fec87b07389a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>A. fumigatus</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>allergens</topic><topic>Allergens - chemistry</topic><topic>Allergens - immunology</topic><topic>Allergic diseases</topic><topic>Antigens, Fungal - chemistry</topic><topic>Antigens, Fungal - immunology</topic><topic>Aspergillus fumigatus</topic><topic>Aspergillus fumigatus - enzymology</topic><topic>Aspergillus fumigatus - immunology</topic><topic>Asthma - immunology</topic><topic>Biological and medical sciences</topic><topic>Cross Reactions</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Humans</topic><topic>immunoblot</topic><topic>Immunoblotting</topic><topic>Immunoglobulin E - immunology</topic><topic>Immunopathology</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>N-terminal amino acid sequence</topic><topic>Penicillium - immunology</topic><topic>Respiratory and ent allergic diseases</topic><topic>Sequence Analysis, Protein</topic><topic>Sequence Homology, Amino Acid</topic><topic>Serine Endopeptidases - chemistry</topic><topic>Serine Endopeptidases - immunology</topic><topic>Vacuoles - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, H.-D.</creatorcontrib><creatorcontrib>Lin, W.-L.</creatorcontrib><creatorcontrib>Tam, M. F.</creatorcontrib><creatorcontrib>Chou, H.</creatorcontrib><creatorcontrib>Wang, C.-W.</creatorcontrib><creatorcontrib>Tsai, J.-J.</creatorcontrib><creatorcontrib>Wang, S.-R.</creatorcontrib><creatorcontrib>Han, S.-H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, H.-D.</au><au>Lin, W.-L.</au><au>Tam, M. F.</au><au>Chou, H.</au><au>Wang, C.-W.</au><au>Tsai, J.-J.</au><au>Wang, S.-R.</au><au>Han, S.-H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N-terminal amino acid sequence analysis</atitle><jtitle>Clinical and experimental allergy</jtitle><addtitle>Clin Exp Allergy</addtitle><date>2001-02</date><risdate>2001</risdate><volume>31</volume><issue>2</issue><spage>295</spage><epage>302</epage><pages>295-302</pages><issn>0954-7894</issn><eissn>1365-2222</eissn><abstract>Aspergillus species are common airborne fungi that have been identified as causative agents of extrinsic bronchial asthma. More than 10 allergens from A. fumigatus have been recently characterized by cDNA cloning.
The objective of this study is to identify A. fumigatus allergens through immunoblot analysis using sera from asthmatic patients.
IgE‐binding components of A. fumigatus and IgE cross‐reactivity among allergens of different prevalent airborne fungal species were analysed by immunoblot and immunoblot inhibition, respectively, using sera from asthmatic patients. The N‐terminal amino acid sequences of major allergens identified were determined by Edman degradation.
Among two batches (70 and 41 sera) of asthmatic sera tested, 19 (27%) and 14 (34%), respectively, have IgE immunoblot reactivity towards components of A. fumigatus. A 34‐kDa protein that reacts with IgE antibodies in 15 (79%) and 11 (79%) of the 19 and 14 positive samples, respectively, may be considered a major allergen of A. fumigatus. The N‐terminal amino acid sequences of the 34 kDa major allergen and the 30.5 and 30 kDa IgE‐binding components of A. fumigatus showed sequence identity to that of the vacuolar serine proteinase from A. fumigatus. The results from immunoblot inhibition show IgE cross‐reactivity among major allergens of A. fumigatus, P. notatum and P. oxalicum.
Results obtained suggest that the 34 kDa major allergen of A. fumigatus may be a vacuolar serine proteinase. There is IgE cross‐reactivity among serine proteinase allergens of A. fumigatus, P. notatum and P. oxalicum.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science, Ltd</pub><pmid>11251631</pmid><doi>10.1046/j.1365-2222.2001.01026.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Clinical and experimental allergy, 2001-02, Vol.31 (2), p.295-302 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | A. fumigatus Adolescent Adult Aged allergens Allergens - chemistry Allergens - immunology Allergic diseases Antigens, Fungal - chemistry Antigens, Fungal - immunology Aspergillus fumigatus Aspergillus fumigatus - enzymology Aspergillus fumigatus - immunology Asthma - immunology Biological and medical sciences Cross Reactions Electrophoresis, Gel, Two-Dimensional Humans immunoblot Immunoblotting Immunoglobulin E - immunology Immunopathology Medical sciences Middle Aged N-terminal amino acid sequence Penicillium - immunology Respiratory and ent allergic diseases Sequence Analysis, Protein Sequence Homology, Amino Acid Serine Endopeptidases - chemistry Serine Endopeptidases - immunology Vacuoles - enzymology |
| title | Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N-terminal amino acid sequence analysis |
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