Complete karyotype characterization of the K562 cell line by combined application of G-banding, multiplex-fluorescence in situ hybridization, fluorescence in situ hybridization, and comparative genomic hybridization
This study combines conventional cytogenetics, fluorescence in situ hybridization (FISH), multiplex-FISH and comparative genomic hybridization (CGH). In applying this multimodal approach on the human leukemia cell line K562, the chromosome composition was refined in detail and compared with data fro...
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| Veröffentlicht in: | Leukemia research 2001-04, Vol.25 (4), p.313-322 |
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| creator | Naumann, Sabine Reutzel, Dirk Speicher, Michael Decker, Hans-Jochen |
| description | This study combines conventional cytogenetics, fluorescence in situ hybridization (FISH), multiplex-FISH and comparative genomic hybridization (CGH). In applying this multimodal approach on the human leukemia cell line K562, the chromosome composition was refined in detail and compared with data from the literature. A hypotriploid karyotype with a modal chromosome number of 67, and 21 unique marker chromosomes were identified. The classification of six markers was identical to published data and the composition of five further markers from the literature could be fully clarified for the first time. The composition of another five markers, which have been interpreted in divergent ways in different studies, were elucidated without doubt. Finally, five new markers of our study seem to have no equivalents in former studies, very likely due to limitations of conventional cytogenetics. The combinatory application of complementary techniques as shown in this study will be very useful to provide the basis of a refined genotype analysis on the chromosomal level. |
| doi_str_mv | 10.1016/S0145-2126(00)00125-9 |
| format | Article |
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In applying this multimodal approach on the human leukemia cell line K562, the chromosome composition was refined in detail and compared with data from the literature. A hypotriploid karyotype with a modal chromosome number of 67, and 21 unique marker chromosomes were identified. The classification of six markers was identical to published data and the composition of five further markers from the literature could be fully clarified for the first time. The composition of another five markers, which have been interpreted in divergent ways in different studies, were elucidated without doubt. Finally, five new markers of our study seem to have no equivalents in former studies, very likely due to limitations of conventional cytogenetics. The combinatory application of complementary techniques as shown in this study will be very useful to provide the basis of a refined genotype analysis on the chromosomal level.</description><identifier>ISSN: 0145-2126</identifier><identifier>EISSN: 1873-5835</identifier><identifier>DOI: 10.1016/S0145-2126(00)00125-9</identifier><identifier>PMID: 11248328</identifier><identifier>CODEN: LEREDD</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biological and medical sciences ; CGH ; Chromosome Mapping - methods ; Chromosome Painting - methods ; CML ; Cytogenetics - methods ; Genetic Markers - genetics ; Hematology ; Humans ; In Situ Hybridization, Fluorescence - methods ; Investigative techniques, diagnostic techniques (general aspects) ; K562 ; K562 Cells - metabolism ; K562 Cells - pathology ; K562 Cells - ultrastructure ; Karyotyping - methods ; M-FISH ; Medical sciences ; Nucleic Acid Hybridization - methods ; Pathology. Cytology. Biochemistry. 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In applying this multimodal approach on the human leukemia cell line K562, the chromosome composition was refined in detail and compared with data from the literature. A hypotriploid karyotype with a modal chromosome number of 67, and 21 unique marker chromosomes were identified. The classification of six markers was identical to published data and the composition of five further markers from the literature could be fully clarified for the first time. The composition of another five markers, which have been interpreted in divergent ways in different studies, were elucidated without doubt. Finally, five new markers of our study seem to have no equivalents in former studies, very likely due to limitations of conventional cytogenetics. The combinatory application of complementary techniques as shown in this study will be very useful to provide the basis of a refined genotype analysis on the chromosomal level.</description><subject>Biological and medical sciences</subject><subject>CGH</subject><subject>Chromosome Mapping - methods</subject><subject>Chromosome Painting - methods</subject><subject>CML</subject><subject>Cytogenetics - methods</subject><subject>Genetic Markers - genetics</subject><subject>Hematology</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>K562</subject><subject>K562 Cells - metabolism</subject><subject>K562 Cells - pathology</subject><subject>K562 Cells - ultrastructure</subject><subject>Karyotyping - methods</subject><subject>M-FISH</subject><subject>Medical sciences</subject><subject>Nucleic Acid Hybridization - methods</subject><subject>Pathology. 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Miscellaneous investigative techniques</subject><issn>0145-2126</issn><issn>1873-5835</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcGK1TAUhosozp3RR1ACgoww1SRtknYlctFRHHChrkOanMyNtk0nSQevL-rrmM4tV1zpKll85_yH7y-KJwS_JJjwV58xqVlJCeXnGL_AmFBWtveKDWlEVbKmYveLzRE5KU5j_IYxZi1pHxYnhNC6qWizKX5t_TD1kAB9V2Hv034CpHcqKJ0guJ8qOT8ib1HaAfrIOEUa-h71bgTU7ZH2Q5e_Bqlp6p0-0pdlp0bjxusLNMx9cjnhR2n72QeIGkYNyI0oujSj3b4LzqxBF-h_mLx5CZ7ykcndArqG0Q9O_409Kh5Y1Ud4vL5nxdd3b79s35dXny4_bN9clZphkUrKTddR0lhLWysq2wI3woA2piZWq7ajTFW049BkY8oYLrjCTGBV8dqA0NVZ8fywdwr-ZoaY5ODi4kiN4OcoBW8F4aLJIDuAOvgYA1g5BTdk55JguTQq7xqVS10SY3nXqGzz3NM1YO4GMH-m1goz8GwFVNSqt0GN2sUj1zLKapqp1wcKsoxbB0FG7RbLxgXQSRrv_nHIb94Cw9Q</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>Naumann, Sabine</creator><creator>Reutzel, Dirk</creator><creator>Speicher, Michael</creator><creator>Decker, Hans-Jochen</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010401</creationdate><title>Complete karyotype characterization of the K562 cell line by combined application of G-banding, multiplex-fluorescence in situ hybridization, fluorescence in situ hybridization, and comparative genomic hybridization</title><author>Naumann, Sabine ; Reutzel, Dirk ; Speicher, Michael ; Decker, Hans-Jochen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-26dbb218ff29f73f9e6d7decdd41fca9b25a32b6e8124add676a0570a364de7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biological and medical sciences</topic><topic>CGH</topic><topic>Chromosome Mapping - methods</topic><topic>Chromosome Painting - methods</topic><topic>CML</topic><topic>Cytogenetics - methods</topic><topic>Genetic Markers - genetics</topic><topic>Hematology</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>K562</topic><topic>K562 Cells - metabolism</topic><topic>K562 Cells - pathology</topic><topic>K562 Cells - ultrastructure</topic><topic>Karyotyping - methods</topic><topic>M-FISH</topic><topic>Medical sciences</topic><topic>Nucleic Acid Hybridization - methods</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naumann, Sabine</creatorcontrib><creatorcontrib>Reutzel, Dirk</creatorcontrib><creatorcontrib>Speicher, Michael</creatorcontrib><creatorcontrib>Decker, Hans-Jochen</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naumann, Sabine</au><au>Reutzel, Dirk</au><au>Speicher, Michael</au><au>Decker, Hans-Jochen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete karyotype characterization of the K562 cell line by combined application of G-banding, multiplex-fluorescence in situ hybridization, fluorescence in situ hybridization, and comparative genomic hybridization</atitle><jtitle>Leukemia research</jtitle><addtitle>Leuk Res</addtitle><date>2001-04-01</date><risdate>2001</risdate><volume>25</volume><issue>4</issue><spage>313</spage><epage>322</epage><pages>313-322</pages><issn>0145-2126</issn><eissn>1873-5835</eissn><coden>LEREDD</coden><abstract>This study combines conventional cytogenetics, fluorescence in situ hybridization (FISH), multiplex-FISH and comparative genomic hybridization (CGH). In applying this multimodal approach on the human leukemia cell line K562, the chromosome composition was refined in detail and compared with data from the literature. A hypotriploid karyotype with a modal chromosome number of 67, and 21 unique marker chromosomes were identified. The classification of six markers was identical to published data and the composition of five further markers from the literature could be fully clarified for the first time. The composition of another five markers, which have been interpreted in divergent ways in different studies, were elucidated without doubt. Finally, five new markers of our study seem to have no equivalents in former studies, very likely due to limitations of conventional cytogenetics. The combinatory application of complementary techniques as shown in this study will be very useful to provide the basis of a refined genotype analysis on the chromosomal level.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11248328</pmid><doi>10.1016/S0145-2126(00)00125-9</doi><tpages>10</tpages></addata></record> |
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| ispartof | Leukemia research, 2001-04, Vol.25 (4), p.313-322 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Biological and medical sciences CGH Chromosome Mapping - methods Chromosome Painting - methods CML Cytogenetics - methods Genetic Markers - genetics Hematology Humans In Situ Hybridization, Fluorescence - methods Investigative techniques, diagnostic techniques (general aspects) K562 K562 Cells - metabolism K562 Cells - pathology K562 Cells - ultrastructure Karyotyping - methods M-FISH Medical sciences Nucleic Acid Hybridization - methods Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques |
| title | Complete karyotype characterization of the K562 cell line by combined application of G-banding, multiplex-fluorescence in situ hybridization, fluorescence in situ hybridization, and comparative genomic hybridization |
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