Cytogenetic abnormalities of alveolar soft-part sarcomas using interphase fluorescent in situ hybridization : trisomy for chromosome 7 and monosomy for chromosomes 8 and 18 seem to be characteristic of the tumor

Four alveolar soft-part sarcomas were investigated by means of standard immunohistochemistry and interphase cytogenetics to further characterize the immunophenotype and proliferative activity of this tumor. The main goal of this study was to explore the chromosomal changes of this rare soft-tissue s...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Virchows Archiv : an international journal of pathology 2001-02, Vol.438 (2), p.173-180
Hauptverfasser: TORNOCZKY, Tamas, KALMAN, Endre, SAPI, Zoltan, OROSZ, Zsolt, PAJOR, Laszlo
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 180
container_issue 2
container_start_page 173
container_title Virchows Archiv : an international journal of pathology
container_volume 438
creator TORNOCZKY, Tamas
KALMAN, Endre
SAPI, Zoltan
OROSZ, Zsolt
PAJOR, Laszlo
description Four alveolar soft-part sarcomas were investigated by means of standard immunohistochemistry and interphase cytogenetics to further characterize the immunophenotype and proliferative activity of this tumor. The main goal of this study was to explore the chromosomal changes of this rare soft-tissue sarcoma. One epithelial (KLI), three neurogenic [neuron specific enolase (NSE), PGP 9.5, and S100], and five myogenic (desmin, myoglobin, alpha-smooth mnuscle actin, alpha-sarcomeric actin, and MyoD1) markers were used for the immunophenotypical analysis. Proliferative activity was assessed using the Ki67 index. Twelve (peri)centromeric (1, 3, 4, 6, 7, 8, 10, 12, 15, 17, 18, and X) and one telomeric (17q25-qtel.) chromosomal probes were used for interphase cytogenetic analysis. Three of the cases showed cytoplasmic desmin and/or myoglobin, and one showed smooth muscle actin positivity. All of the four tumors had granular, cytoplasmic, possibly nonspecific MyoD1 and sarcomeric actin positivity. Two of the tumors were positive for vimentin, four gave focal and weak staining with neurogenic markers (four of four NSE, one of four S100, and four of four PGP 9.5), but none of them was positive with KLI. Alveolar soft-part sarcomas may show myogenic immunophenotype in a number of cases, which supports myogenic differentiation. Fluorescent in situ hybridization using alpha satellite chromosomal probes revealed significant alterations in all of the cases. Most frequent and repeated numerical changes, which seem to be characteristic of the neoplasm and may play an important part in its pathogenesis and/or progression, were trisomy 7, monosomy 8 and monosomy 18.
doi_str_mv 10.1007/s004280000332
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76969457</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76969457</sourcerecordid><originalsourceid>FETCH-LOGICAL-c317t-86d1bf2989ad6a8b8ba7a87981202a6cd19b345aee256db9c8ad8bca537d67f73</originalsourceid><addsrcrecordid>eNptkUurFDEQhYMo3vHq0q0UCO5a85hJp93J4AsuuNF1U0mn70Q6yZhKC-Pf9A-Z8Q6KYDYhdT6qTuow9lTwl4Lz_hVxvpWGt6OUvMc2YqtkJxXv77MNH7a7TivRX7FHRF85l8II_ZBdCSF3Ski-YT_3p5pvffI1OECbcom4hBo8QZ4Bl-8-L1iA8ly7I5YKhMXliAQrhXQLIVVfjgckD_Oy5uLJ-VRbGSjUFQ4nW8IUfmANOcFrqCVQjieYcwF3KDnm9vTQA6YJYk75PyqB-S0LA-R9hJrB-qZjQdeGBzpbb2brwUNdYy6P2YMZF_JPLvc1-_Lu7ef9h-7m0_uP-zc3nWsrqZ3Rk7CzHMyAk0ZjjcUeTT-YthiJ2k1isGq7Q-_lTk92cAYnYx3uVD_pfu7VNXtx1_dY8rfVUx1jaN9fFkw-rzT2etAtgTPY3YGuZKLi5_FYQsRyGgUfzymO_6TY-GeXxquNfvpLX2JrwPMLgORwmQsmF-gPZwatBql-AQ1Eqd0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76969457</pqid></control><display><type>article</type><title>Cytogenetic abnormalities of alveolar soft-part sarcomas using interphase fluorescent in situ hybridization : trisomy for chromosome 7 and monosomy for chromosomes 8 and 18 seem to be characteristic of the tumor</title><source>MEDLINE</source><source>Springer Online Journals Complete</source><creator>TORNOCZKY, Tamas ; KALMAN, Endre ; SAPI, Zoltan ; OROSZ, Zsolt ; PAJOR, Laszlo</creator><creatorcontrib>TORNOCZKY, Tamas ; KALMAN, Endre ; SAPI, Zoltan ; OROSZ, Zsolt ; PAJOR, Laszlo</creatorcontrib><description>Four alveolar soft-part sarcomas were investigated by means of standard immunohistochemistry and interphase cytogenetics to further characterize the immunophenotype and proliferative activity of this tumor. The main goal of this study was to explore the chromosomal changes of this rare soft-tissue sarcoma. One epithelial (KLI), three neurogenic [neuron specific enolase (NSE), PGP 9.5, and S100], and five myogenic (desmin, myoglobin, alpha-smooth mnuscle actin, alpha-sarcomeric actin, and MyoD1) markers were used for the immunophenotypical analysis. Proliferative activity was assessed using the Ki67 index. Twelve (peri)centromeric (1, 3, 4, 6, 7, 8, 10, 12, 15, 17, 18, and X) and one telomeric (17q25-qtel.) chromosomal probes were used for interphase cytogenetic analysis. Three of the cases showed cytoplasmic desmin and/or myoglobin, and one showed smooth muscle actin positivity. All of the four tumors had granular, cytoplasmic, possibly nonspecific MyoD1 and sarcomeric actin positivity. Two of the tumors were positive for vimentin, four gave focal and weak staining with neurogenic markers (four of four NSE, one of four S100, and four of four PGP 9.5), but none of them was positive with KLI. Alveolar soft-part sarcomas may show myogenic immunophenotype in a number of cases, which supports myogenic differentiation. Fluorescent in situ hybridization using alpha satellite chromosomal probes revealed significant alterations in all of the cases. Most frequent and repeated numerical changes, which seem to be characteristic of the neoplasm and may play an important part in its pathogenesis and/or progression, were trisomy 7, monosomy 8 and monosomy 18.</description><identifier>ISSN: 0945-6317</identifier><identifier>EISSN: 1432-2307</identifier><identifier>DOI: 10.1007/s004280000332</identifier><identifier>PMID: 11253120</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Adolescent ; Adult ; Biological and medical sciences ; Biomarkers, Tumor - analysis ; Chromosomes, Human, Pair 18 ; Chromosomes, Human, Pair 7 ; Chromosomes, Human, Pair 8 ; Dermatology ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Interphase ; Male ; Medical sciences ; Middle Aged ; Neoplasm Proteins - analysis ; Sarcoma, Alveolar Soft Part - chemistry ; Sarcoma, Alveolar Soft Part - genetics ; Sarcoma, Alveolar Soft Part - pathology ; Soft Tissue Neoplasms - chemistry ; Soft Tissue Neoplasms - genetics ; Soft Tissue Neoplasms - pathology ; Trisomy ; Tumors of the skin and soft tissue. Premalignant lesions</subject><ispartof>Virchows Archiv : an international journal of pathology, 2001-02, Vol.438 (2), p.173-180</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c317t-86d1bf2989ad6a8b8ba7a87981202a6cd19b345aee256db9c8ad8bca537d67f73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=896392$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11253120$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TORNOCZKY, Tamas</creatorcontrib><creatorcontrib>KALMAN, Endre</creatorcontrib><creatorcontrib>SAPI, Zoltan</creatorcontrib><creatorcontrib>OROSZ, Zsolt</creatorcontrib><creatorcontrib>PAJOR, Laszlo</creatorcontrib><title>Cytogenetic abnormalities of alveolar soft-part sarcomas using interphase fluorescent in situ hybridization : trisomy for chromosome 7 and monosomy for chromosomes 8 and 18 seem to be characteristic of the tumor</title><title>Virchows Archiv : an international journal of pathology</title><addtitle>Virchows Arch</addtitle><description>Four alveolar soft-part sarcomas were investigated by means of standard immunohistochemistry and interphase cytogenetics to further characterize the immunophenotype and proliferative activity of this tumor. The main goal of this study was to explore the chromosomal changes of this rare soft-tissue sarcoma. One epithelial (KLI), three neurogenic [neuron specific enolase (NSE), PGP 9.5, and S100], and five myogenic (desmin, myoglobin, alpha-smooth mnuscle actin, alpha-sarcomeric actin, and MyoD1) markers were used for the immunophenotypical analysis. Proliferative activity was assessed using the Ki67 index. Twelve (peri)centromeric (1, 3, 4, 6, 7, 8, 10, 12, 15, 17, 18, and X) and one telomeric (17q25-qtel.) chromosomal probes were used for interphase cytogenetic analysis. Three of the cases showed cytoplasmic desmin and/or myoglobin, and one showed smooth muscle actin positivity. All of the four tumors had granular, cytoplasmic, possibly nonspecific MyoD1 and sarcomeric actin positivity. Two of the tumors were positive for vimentin, four gave focal and weak staining with neurogenic markers (four of four NSE, one of four S100, and four of four PGP 9.5), but none of them was positive with KLI. Alveolar soft-part sarcomas may show myogenic immunophenotype in a number of cases, which supports myogenic differentiation. Fluorescent in situ hybridization using alpha satellite chromosomal probes revealed significant alterations in all of the cases. Most frequent and repeated numerical changes, which seem to be characteristic of the neoplasm and may play an important part in its pathogenesis and/or progression, were trisomy 7, monosomy 8 and monosomy 18.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Chromosomes, Human, Pair 18</subject><subject>Chromosomes, Human, Pair 7</subject><subject>Chromosomes, Human, Pair 8</subject><subject>Dermatology</subject><subject>Female</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Interphase</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Proteins - analysis</subject><subject>Sarcoma, Alveolar Soft Part - chemistry</subject><subject>Sarcoma, Alveolar Soft Part - genetics</subject><subject>Sarcoma, Alveolar Soft Part - pathology</subject><subject>Soft Tissue Neoplasms - chemistry</subject><subject>Soft Tissue Neoplasms - genetics</subject><subject>Soft Tissue Neoplasms - pathology</subject><subject>Trisomy</subject><subject>Tumors of the skin and soft tissue. Premalignant lesions</subject><issn>0945-6317</issn><issn>1432-2307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUurFDEQhYMo3vHq0q0UCO5a85hJp93J4AsuuNF1U0mn70Q6yZhKC-Pf9A-Z8Q6KYDYhdT6qTuow9lTwl4Lz_hVxvpWGt6OUvMc2YqtkJxXv77MNH7a7TivRX7FHRF85l8II_ZBdCSF3Ski-YT_3p5pvffI1OECbcom4hBo8QZ4Bl-8-L1iA8ly7I5YKhMXliAQrhXQLIVVfjgckD_Oy5uLJ-VRbGSjUFQ4nW8IUfmANOcFrqCVQjieYcwF3KDnm9vTQA6YJYk75PyqB-S0LA-R9hJrB-qZjQdeGBzpbb2brwUNdYy6P2YMZF_JPLvc1-_Lu7ef9h-7m0_uP-zc3nWsrqZ3Rk7CzHMyAk0ZjjcUeTT-YthiJ2k1isGq7Q-_lTk92cAYnYx3uVD_pfu7VNXtx1_dY8rfVUx1jaN9fFkw-rzT2etAtgTPY3YGuZKLi5_FYQsRyGgUfzymO_6TY-GeXxquNfvpLX2JrwPMLgORwmQsmF-gPZwatBql-AQ1Eqd0</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>TORNOCZKY, Tamas</creator><creator>KALMAN, Endre</creator><creator>SAPI, Zoltan</creator><creator>OROSZ, Zsolt</creator><creator>PAJOR, Laszlo</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010201</creationdate><title>Cytogenetic abnormalities of alveolar soft-part sarcomas using interphase fluorescent in situ hybridization : trisomy for chromosome 7 and monosomy for chromosomes 8 and 18 seem to be characteristic of the tumor</title><author>TORNOCZKY, Tamas ; KALMAN, Endre ; SAPI, Zoltan ; OROSZ, Zsolt ; PAJOR, Laszlo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c317t-86d1bf2989ad6a8b8ba7a87981202a6cd19b345aee256db9c8ad8bca537d67f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Chromosomes, Human, Pair 18</topic><topic>Chromosomes, Human, Pair 7</topic><topic>Chromosomes, Human, Pair 8</topic><topic>Dermatology</topic><topic>Female</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Interphase</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasm Proteins - analysis</topic><topic>Sarcoma, Alveolar Soft Part - chemistry</topic><topic>Sarcoma, Alveolar Soft Part - genetics</topic><topic>Sarcoma, Alveolar Soft Part - pathology</topic><topic>Soft Tissue Neoplasms - chemistry</topic><topic>Soft Tissue Neoplasms - genetics</topic><topic>Soft Tissue Neoplasms - pathology</topic><topic>Trisomy</topic><topic>Tumors of the skin and soft tissue. Premalignant lesions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TORNOCZKY, Tamas</creatorcontrib><creatorcontrib>KALMAN, Endre</creatorcontrib><creatorcontrib>SAPI, Zoltan</creatorcontrib><creatorcontrib>OROSZ, Zsolt</creatorcontrib><creatorcontrib>PAJOR, Laszlo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virchows Archiv : an international journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TORNOCZKY, Tamas</au><au>KALMAN, Endre</au><au>SAPI, Zoltan</au><au>OROSZ, Zsolt</au><au>PAJOR, Laszlo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytogenetic abnormalities of alveolar soft-part sarcomas using interphase fluorescent in situ hybridization : trisomy for chromosome 7 and monosomy for chromosomes 8 and 18 seem to be characteristic of the tumor</atitle><jtitle>Virchows Archiv : an international journal of pathology</jtitle><addtitle>Virchows Arch</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>438</volume><issue>2</issue><spage>173</spage><epage>180</epage><pages>173-180</pages><issn>0945-6317</issn><eissn>1432-2307</eissn><abstract>Four alveolar soft-part sarcomas were investigated by means of standard immunohistochemistry and interphase cytogenetics to further characterize the immunophenotype and proliferative activity of this tumor. The main goal of this study was to explore the chromosomal changes of this rare soft-tissue sarcoma. One epithelial (KLI), three neurogenic [neuron specific enolase (NSE), PGP 9.5, and S100], and five myogenic (desmin, myoglobin, alpha-smooth mnuscle actin, alpha-sarcomeric actin, and MyoD1) markers were used for the immunophenotypical analysis. Proliferative activity was assessed using the Ki67 index. Twelve (peri)centromeric (1, 3, 4, 6, 7, 8, 10, 12, 15, 17, 18, and X) and one telomeric (17q25-qtel.) chromosomal probes were used for interphase cytogenetic analysis. Three of the cases showed cytoplasmic desmin and/or myoglobin, and one showed smooth muscle actin positivity. All of the four tumors had granular, cytoplasmic, possibly nonspecific MyoD1 and sarcomeric actin positivity. Two of the tumors were positive for vimentin, four gave focal and weak staining with neurogenic markers (four of four NSE, one of four S100, and four of four PGP 9.5), but none of them was positive with KLI. Alveolar soft-part sarcomas may show myogenic immunophenotype in a number of cases, which supports myogenic differentiation. Fluorescent in situ hybridization using alpha satellite chromosomal probes revealed significant alterations in all of the cases. Most frequent and repeated numerical changes, which seem to be characteristic of the neoplasm and may play an important part in its pathogenesis and/or progression, were trisomy 7, monosomy 8 and monosomy 18.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>11253120</pmid><doi>10.1007/s004280000332</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0945-6317
ispartof Virchows Archiv : an international journal of pathology, 2001-02, Vol.438 (2), p.173-180
issn 0945-6317
1432-2307
language eng
recordid cdi_proquest_miscellaneous_76969457
source MEDLINE; Springer Online Journals Complete
subjects Adolescent
Adult
Biological and medical sciences
Biomarkers, Tumor - analysis
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 7
Chromosomes, Human, Pair 8
Dermatology
Female
Humans
In Situ Hybridization, Fluorescence
Interphase
Male
Medical sciences
Middle Aged
Neoplasm Proteins - analysis
Sarcoma, Alveolar Soft Part - chemistry
Sarcoma, Alveolar Soft Part - genetics
Sarcoma, Alveolar Soft Part - pathology
Soft Tissue Neoplasms - chemistry
Soft Tissue Neoplasms - genetics
Soft Tissue Neoplasms - pathology
Trisomy
Tumors of the skin and soft tissue. Premalignant lesions
title Cytogenetic abnormalities of alveolar soft-part sarcomas using interphase fluorescent in situ hybridization : trisomy for chromosome 7 and monosomy for chromosomes 8 and 18 seem to be characteristic of the tumor
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T10%3A05%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cytogenetic%20abnormalities%20of%20alveolar%20soft-part%20sarcomas%20using%20interphase%20fluorescent%20in%20situ%20hybridization%20:%20trisomy%20for%20chromosome%207%20and%20monosomy%20for%20chromosomes%208%20and%2018%20seem%20to%20be%20characteristic%20of%20the%20tumor&rft.jtitle=Virchows%20Archiv%20:%20an%20international%20journal%20of%20pathology&rft.au=TORNOCZKY,%20Tamas&rft.date=2001-02-01&rft.volume=438&rft.issue=2&rft.spage=173&rft.epage=180&rft.pages=173-180&rft.issn=0945-6317&rft.eissn=1432-2307&rft_id=info:doi/10.1007/s004280000332&rft_dat=%3Cproquest_cross%3E76969457%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76969457&rft_id=info:pmid/11253120&rfr_iscdi=true