Derivation of ganglioside-specific T cell lines of suppressor or helper phenotype from cerebrospinal fluid of multiple sclerosis patients
In order to investigate the specificity of activated T cells in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS), we have cultured cells in the presence of mitogen-free IL-2 but without any antigen. Two T cell lines have been derived and showed specific reactivity to certain pu...
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Veröffentlicht in: | Journal of neuroimmunology 1986-08, Vol.12 (2), p.107-120 |
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Sprache: | eng |
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Zusammenfassung: | In order to investigate the specificity of activated T cells in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS), we have cultured cells in the presence of mitogen-free IL-2 but without any antigen. Two T cell lines have been derived and showed specific reactivity to certain purified gangliosides (G
M1, G
D1a, G
D1b and G
Q1b). However, responses to other brain and viral antigens were not seen, and neither were T cell lines from peripheral blood lymphocytes (PBL) of normal, MS or other neurological disease patients stimulated by these gangliosides. Release of IL-2 could be detected after incubation of these CSF lines with specific gangliosides. One line exhibited predominantly helper/inducer (T4
+) phenotype whilst the other was suppressor/cytotoxic (T8
+), and further analysis indicated it could be of the suppressor phenotype. These data may have implications for T cell-induced demyelination in MS. |
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ISSN: | 0165-5728 1872-8421 |
DOI: | 10.1016/0165-5728(86)90024-X |