Derivation of ganglioside-specific T cell lines of suppressor or helper phenotype from cerebrospinal fluid of multiple sclerosis patients

In order to investigate the specificity of activated T cells in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS), we have cultured cells in the presence of mitogen-free IL-2 but without any antigen. Two T cell lines have been derived and showed specific reactivity to certain pu...

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Veröffentlicht in:Journal of neuroimmunology 1986-08, Vol.12 (2), p.107-120
Hauptverfasser: Bellamy, A., Davison, A.N., Feldmann, M.
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Sprache:eng
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Zusammenfassung:In order to investigate the specificity of activated T cells in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS), we have cultured cells in the presence of mitogen-free IL-2 but without any antigen. Two T cell lines have been derived and showed specific reactivity to certain purified gangliosides (G M1, G D1a, G D1b and G Q1b). However, responses to other brain and viral antigens were not seen, and neither were T cell lines from peripheral blood lymphocytes (PBL) of normal, MS or other neurological disease patients stimulated by these gangliosides. Release of IL-2 could be detected after incubation of these CSF lines with specific gangliosides. One line exhibited predominantly helper/inducer (T4 +) phenotype whilst the other was suppressor/cytotoxic (T8 +), and further analysis indicated it could be of the suppressor phenotype. These data may have implications for T cell-induced demyelination in MS.
ISSN:0165-5728
1872-8421
DOI:10.1016/0165-5728(86)90024-X