Comparative Analysis of Human DNA Variations by Fluorescence-Based Sequencing of PCR Products

Automated, direct cycle sequencing of purified double-stranded PCR products using Taq polymerase and fluorescently labeled dideoxynucleotide terminators provides a robust and highly reproducible method for identifying DNA sequence variations in sequence-tagged sites. We describe a simple and sensiti...

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Veröffentlicht in:	Genomics 1994-09, Vol.23 (1), p.138-144
Hauptverfasser:	Kwok, Pui-Yan, Carlson, Christopher, Yager, Thomas D., Ankener, Wendy, Nickerson, Deborah A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles AUTOMATION Base Sequence Biological and medical sciences BIOLOGICAL EVOLUTION BIOLOGY AND MEDICINE, BASIC STUDIES DETECTION Diverse techniques DNA DNA - genetics DNA SEQUENCERS DNA SEQUENCING DNA-Directed DNA Polymerase EVALUATION FLUORESCENCE Fluorometry Fundamental and applied biological sciences. Psychology Gene Frequency GENE MUTATIONS GENES GENETIC VARIABILITY Genetic Variation GENOTYPE Humans INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS Molecular and cellular biology Molecular Sequence Data MUTATION FREQUENCY NUCLEOTIDES POLYMERASE CHAIN REACTION RELIABILITY Reproducibility of Results Sequence Analysis, DNA - methods STATISTICS Taq Polymerase
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