Monitoring the activity of glucose oxidase during the cultivation of Aspergillus niger using novel amperometric sensor with 1, 1′-dimethylferricinium as a mediator
1, 1′-dimethylferricinium (DMF +), a deep blue, and stable mediator, was prepared from a water-soluble 1, 1′-dimethylferrocene(DMF):2-hydroxypropyl-β-cyclodextrin complex via enzymatic oxidation using immobilised bilirubin oxidase. This mediator was superior to other soluble ferrocenes, notably carb...
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| Veröffentlicht in: | Biosensors & bioelectronics 1994, Vol.9 (8), p.577-584 |
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| creator | Luong, J.H.T. Masson, C. Brown, R.S. Male, K.B. Nguyen, A.-L. |
| description | 1, 1′-dimethylferricinium (DMF
+), a deep blue, and stable mediator, was prepared from a water-soluble 1, 1′-dimethylferrocene(DMF):2-hydroxypropyl-β-cyclodextrin complex via enzymatic oxidation using immobilised bilirubin oxidase. This mediator was superior to other soluble ferrocenes, notably carboxyferrocene, in terms of both solubility (110 mM
vs 0·5 mM) and oxidation potential (150 mV
vs 300 mV against Ag/AgCl). Although the cyclic voltammogram of DMF
+ was electrochemically equivalent to DMF, the use of the former resulted in a significantly lower background current ( |
| doi_str_mv | 10.1016/0956-5663(94)80050-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76954877</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0956566394800502</els_id><sourcerecordid>16918167</sourcerecordid><originalsourceid>FETCH-LOGICAL-c484t-41212a74724a60cbc9945cb03163e6b2cde2689dcd35296de9876f9e70b6c98e3</originalsourceid><addsrcrecordid>eNqN0c2KFDEQB_BGlHV29Q0UchBZwdak89HJZWFZ_IIVL3oO6XT1TCTdGZP06Nx8GV_AR_JJTDvDHNVTHepXRVH_qnpE8AuCiXiJFRc1F4JeKvZMYsxx3dypVkS2tGYN5Xer1Yncr85T-owxbonCZ9VZKxvBJVlVP96HyeUQ3bRGeQPI2Ox2Lu9RGNDazzYkQOGb602p_XxidvbFmezCtMjrtIW4dt7PCU1uDRHNaaFT2IFHZizdMEKOzqIEUwoRfXV5g8hzRH59_1n3rjQ3ez9ALMRNbh6RScigEXpnynUPqnuD8QkeHutF9en1q483b-vbD2_e3Vzf1pZJlmtGGtKYlrUNMwLbzirFuO0wJYKC6BrbQyOk6m1PeaNED0q2YlDQ4k5YJYFeVE8Pe7cxfJkhZT26ZMF7M0GYk26F4ky27T8hEYpIIv4HcoUl4wWyA7QxpBRh0NvoRhP3mmC95K2XMPUSplZM_8lbN2Xs8XH_3JVvnYaOAZf-k2PfJGv8EM1kXToxSgllTBR2dWBQvrtzEHWyDiZb_h_BZt0H9_c7fgNubsqW</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16590845</pqid></control><display><type>article</type><title>Monitoring the activity of glucose oxidase during the cultivation of Aspergillus niger using novel amperometric sensor with 1, 1′-dimethylferricinium as a mediator</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Luong, J.H.T. ; Masson, C. ; Brown, R.S. ; Male, K.B. ; Nguyen, A.-L.</creator><creatorcontrib>Luong, J.H.T. ; Masson, C. ; Brown, R.S. ; Male, K.B. ; Nguyen, A.-L.</creatorcontrib><description>1, 1′-dimethylferricinium (DMF
+), a deep blue, and stable mediator, was prepared from a water-soluble 1, 1′-dimethylferrocene(DMF):2-hydroxypropyl-β-cyclodextrin complex via enzymatic oxidation using immobilised bilirubin oxidase. This mediator was superior to other soluble ferrocenes, notably carboxyferrocene, in terms of both solubility (110 mM
vs 0·5 mM) and oxidation potential (150 mV
vs 300 mV against Ag/AgCl). Although the cyclic voltammogram of DMF
+ was electrochemically equivalent to DMF, the use of the former resulted in a significantly lower background current (<10 nA
vs 30 nA). Because of its higher solubility, concentrated stock solutions of DMF
+ can be prepared and supplied to the electrode. This is of particular importance when the signal is severely limited by the rate at which the working electrode can oxidise DMF to DMF
+.
A linear response of current versus units of glucose oxidase (GOD) was obtained up to 0·5 unit/ml. The detection limit was estimated to be 0·03 unit/ml and the response time was 2·5 min or less. The amperometric system was used successfully to follow the GOD activity during the growth of
Aspergillus niger a well-known GOD producer. The results obtained correlated well with a standard absorbance-based assay using dichlorophenol-indophenol (DCPIP). The K
M of GOD for the glucose in the lysate was measured as 38 mM. A reduced response and higher K
M (48 mM) of the cell homogenate, compared to the lysate, illustrated the requirement for the DMF
+ and glucose to diffuse across the cell membrane to interact with GOD in whole cells.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/0956-5663(94)80050-2</identifier><identifier>PMID: 7826581</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>1′-dimethylferricinium ; 1′-dimethylferrocene ; 2-hydroxypropyl-β-cyclodextrin ; amperometric ; Aspergillus niger ; Aspergillus niger - enzymology ; Biological and medical sciences ; Biosensing Techniques ; Biosensors ; Biotechnology ; Electrochemistry ; enzyme activity ; Fundamental and applied biological sciences. Psychology ; glucose ; glucose oxidase ; Glucose Oxidase - metabolism ; Methods. Procedures. Technologies ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 1994, Vol.9 (8), p.577-584</ispartof><rights>1994</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-41212a74724a60cbc9945cb03163e6b2cde2689dcd35296de9876f9e70b6c98e3</citedby><cites>FETCH-LOGICAL-c484t-41212a74724a60cbc9945cb03163e6b2cde2689dcd35296de9876f9e70b6c98e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0956-5663(94)80050-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3313446$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7826581$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luong, J.H.T.</creatorcontrib><creatorcontrib>Masson, C.</creatorcontrib><creatorcontrib>Brown, R.S.</creatorcontrib><creatorcontrib>Male, K.B.</creatorcontrib><creatorcontrib>Nguyen, A.-L.</creatorcontrib><title>Monitoring the activity of glucose oxidase during the cultivation of Aspergillus niger using novel amperometric sensor with 1, 1′-dimethylferricinium as a mediator</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>1, 1′-dimethylferricinium (DMF
+), a deep blue, and stable mediator, was prepared from a water-soluble 1, 1′-dimethylferrocene(DMF):2-hydroxypropyl-β-cyclodextrin complex via enzymatic oxidation using immobilised bilirubin oxidase. This mediator was superior to other soluble ferrocenes, notably carboxyferrocene, in terms of both solubility (110 mM
vs 0·5 mM) and oxidation potential (150 mV
vs 300 mV against Ag/AgCl). Although the cyclic voltammogram of DMF
+ was electrochemically equivalent to DMF, the use of the former resulted in a significantly lower background current (<10 nA
vs 30 nA). Because of its higher solubility, concentrated stock solutions of DMF
+ can be prepared and supplied to the electrode. This is of particular importance when the signal is severely limited by the rate at which the working electrode can oxidise DMF to DMF
+.
A linear response of current versus units of glucose oxidase (GOD) was obtained up to 0·5 unit/ml. The detection limit was estimated to be 0·03 unit/ml and the response time was 2·5 min or less. The amperometric system was used successfully to follow the GOD activity during the growth of
Aspergillus niger a well-known GOD producer. The results obtained correlated well with a standard absorbance-based assay using dichlorophenol-indophenol (DCPIP). The K
M of GOD for the glucose in the lysate was measured as 38 mM. A reduced response and higher K
M (48 mM) of the cell homogenate, compared to the lysate, illustrated the requirement for the DMF
+ and glucose to diffuse across the cell membrane to interact with GOD in whole cells.</description><subject>1′-dimethylferricinium</subject><subject>1′-dimethylferrocene</subject><subject>2-hydroxypropyl-β-cyclodextrin</subject><subject>amperometric</subject><subject>Aspergillus niger</subject><subject>Aspergillus niger - enzymology</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Electrochemistry</subject><subject>enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glucose</subject><subject>glucose oxidase</subject><subject>Glucose Oxidase - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0c2KFDEQB_BGlHV29Q0UchBZwdak89HJZWFZ_IIVL3oO6XT1TCTdGZP06Nx8GV_AR_JJTDvDHNVTHepXRVH_qnpE8AuCiXiJFRc1F4JeKvZMYsxx3dypVkS2tGYN5Xer1Yncr85T-owxbonCZ9VZKxvBJVlVP96HyeUQ3bRGeQPI2Ox2Lu9RGNDazzYkQOGb602p_XxidvbFmezCtMjrtIW4dt7PCU1uDRHNaaFT2IFHZizdMEKOzqIEUwoRfXV5g8hzRH59_1n3rjQ3ez9ALMRNbh6RScigEXpnynUPqnuD8QkeHutF9en1q483b-vbD2_e3Vzf1pZJlmtGGtKYlrUNMwLbzirFuO0wJYKC6BrbQyOk6m1PeaNED0q2YlDQ4k5YJYFeVE8Pe7cxfJkhZT26ZMF7M0GYk26F4ky27T8hEYpIIv4HcoUl4wWyA7QxpBRh0NvoRhP3mmC95K2XMPUSplZM_8lbN2Xs8XH_3JVvnYaOAZf-k2PfJGv8EM1kXToxSgllTBR2dWBQvrtzEHWyDiZb_h_BZt0H9_c7fgNubsqW</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Luong, J.H.T.</creator><creator>Masson, C.</creator><creator>Brown, R.S.</creator><creator>Male, K.B.</creator><creator>Nguyen, A.-L.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7TC</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Monitoring the activity of glucose oxidase during the cultivation of Aspergillus niger using novel amperometric sensor with 1, 1′-dimethylferricinium as a mediator</title><author>Luong, J.H.T. ; Masson, C. ; Brown, R.S. ; Male, K.B. ; Nguyen, A.-L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-41212a74724a60cbc9945cb03163e6b2cde2689dcd35296de9876f9e70b6c98e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>1′-dimethylferricinium</topic><topic>1′-dimethylferrocene</topic><topic>2-hydroxypropyl-β-cyclodextrin</topic><topic>amperometric</topic><topic>Aspergillus niger</topic><topic>Aspergillus niger - enzymology</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Electrochemistry</topic><topic>enzyme activity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glucose</topic><topic>glucose oxidase</topic><topic>Glucose Oxidase - metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luong, J.H.T.</creatorcontrib><creatorcontrib>Masson, C.</creatorcontrib><creatorcontrib>Brown, R.S.</creatorcontrib><creatorcontrib>Male, K.B.</creatorcontrib><creatorcontrib>Nguyen, A.-L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Mechanical Engineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luong, J.H.T.</au><au>Masson, C.</au><au>Brown, R.S.</au><au>Male, K.B.</au><au>Nguyen, A.-L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring the activity of glucose oxidase during the cultivation of Aspergillus niger using novel amperometric sensor with 1, 1′-dimethylferricinium as a mediator</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>1994</date><risdate>1994</risdate><volume>9</volume><issue>8</issue><spage>577</spage><epage>584</epage><pages>577-584</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>1, 1′-dimethylferricinium (DMF
+), a deep blue, and stable mediator, was prepared from a water-soluble 1, 1′-dimethylferrocene(DMF):2-hydroxypropyl-β-cyclodextrin complex via enzymatic oxidation using immobilised bilirubin oxidase. This mediator was superior to other soluble ferrocenes, notably carboxyferrocene, in terms of both solubility (110 mM
vs 0·5 mM) and oxidation potential (150 mV
vs 300 mV against Ag/AgCl). Although the cyclic voltammogram of DMF
+ was electrochemically equivalent to DMF, the use of the former resulted in a significantly lower background current (<10 nA
vs 30 nA). Because of its higher solubility, concentrated stock solutions of DMF
+ can be prepared and supplied to the electrode. This is of particular importance when the signal is severely limited by the rate at which the working electrode can oxidise DMF to DMF
+.
A linear response of current versus units of glucose oxidase (GOD) was obtained up to 0·5 unit/ml. The detection limit was estimated to be 0·03 unit/ml and the response time was 2·5 min or less. The amperometric system was used successfully to follow the GOD activity during the growth of
Aspergillus niger a well-known GOD producer. The results obtained correlated well with a standard absorbance-based assay using dichlorophenol-indophenol (DCPIP). The K
M of GOD for the glucose in the lysate was measured as 38 mM. A reduced response and higher K
M (48 mM) of the cell homogenate, compared to the lysate, illustrated the requirement for the DMF
+ and glucose to diffuse across the cell membrane to interact with GOD in whole cells.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>7826581</pmid><doi>10.1016/0956-5663(94)80050-2</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biosensors & bioelectronics, 1994, Vol.9 (8), p.577-584 |
| issn | 0956-5663 1873-4235 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 1′-dimethylferricinium 1′-dimethylferrocene 2-hydroxypropyl-β-cyclodextrin amperometric Aspergillus niger Aspergillus niger - enzymology Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Electrochemistry enzyme activity Fundamental and applied biological sciences. Psychology glucose glucose oxidase Glucose Oxidase - metabolism Methods. Procedures. Technologies Various methods and equipments |
| title | Monitoring the activity of glucose oxidase during the cultivation of Aspergillus niger using novel amperometric sensor with 1, 1′-dimethylferricinium as a mediator |
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