The Bacillus subtilis pnbA gene encoding p-nitrobenzyl esterase: cloning, sequence and high-level expression in Escherichia coli

p-Nitrobenzyl esters serve as protecting groups on intermediates in the manufacture of clinically important oral β-lactam antibiotics; de-esterification of the intermediates is required for synthesis of the final product. A Bacillus subtilis PNB carboxy-esterase (PNBCE) catalyzes hydrolysis of sever...

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Veröffentlicht in:	Gene 1994-12, Vol.151 (1-2), p.37-43
Hauptverfasser:	Zock, Joseph, Cantwell, Cathleen, Swartling, James, Hodges, Roland, Pohl, Tonya, Sutton, Kimberly, Rosteck, Paul, McGilvray, Derek, Queener, Stephen
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Anti-Bacterial Agents - metabolism Bacillus subtilis Bacillus subtilis - enzymology Bacillus subtilis - genetics Base Sequence Biocatalysis Carboxylic Ester Hydrolases - biosynthesis Carboxylic Ester Hydrolases - genetics Carboxylic Ester Hydrolases - metabolism Cloning, Molecular de-esterification Electrophoresis, Polyacrylamide Gel Enzyme Induction Escherichia coli Gene Expression Genes, Bacterial heterologous expression Kinetics Lactams Molecular Sequence Data Molecular Weight nucleotide sequence Oligonucleotide Probes Plasmids Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Restriction Mapping Substrate Specificity Thermodynamics β-lactam antibiotic carboxy-esters
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